RegulonDB RegulonDB 10.10: Operon Form
   

folE-yeiB operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: folE-yeiB
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: folE-yeiB
Gene(s): yeiB, folE   Genome Browser M3D Gene expression COLOMBOS
Evidence: [ICWHO] Inferred computationally without human oversight
Promoter
Name: folEp
+1: 2243848
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 196
Sequence: cgtggctccttgttgtgttgtttgcaatttcttcatcataccgtttattcaatgtgctgtGagtaactttcacttccgtat
                      -35                     -10           +1                   
Evidence: [ICA]
[ICWHO]
Reference(s): [1] Huerta AM., et al., 2003
TF binding sites (TFBSs)
[MetJ,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote MetJ-S-adenosyl-L-methionine repressor folEp 2243792 2243807 49.5 atgttttaacATCTGCTGATGAAAGGcagcggcaat nd [GEA], [AIBSCS], [BPP] [4]
remote MetJ-S-adenosyl-L-methionine repressor folEp 2243800 2243815 41.5 gcataacgatGTTTTAACATCTGCTGatgaaaggca nd [GEA], [AIBSCS], [BPP] [4]
remote MetJ-S-adenosyl-L-methionine repressor folEp 2243808 2243823 33.5 ccgtatttgcATAACGATGTTTTAACatctgctgat nd [GEA], [AIBSCS], [BPP] [4]
proximal MetJ-S-adenosyl-L-methionine repressor folEp 2243816 2243831 25.5 tttcacttccGTATTTGCATAACGATgttttaacat nd [GEA], [AIBSCS], [BPP] [4]
sRNA Interaction TU
sRNA TU Regulated Function Binding Sites Regulatory Mechanism Evidence (Confirmed, Strong, Weak) Reference(s)
PosLeft PosRight Target sequence (mRNA)
small regulatory RNA SgrS folE-yeiB repressor 2243664 2243677 CCUGCAGGUGUGAC TRANSLATION-BLOCKING, MRNA-DEGRADATION [IMP], [SM] [2]
small regulatory RNA FnrS folE-yeiB repressor 2243667 2243679 GCAGGUGUGACGC nd [IEP], [IMP] [3]


Evidence    

 [ICA] Inferred by computational analysis

 [ICWHO] Inferred computationally without human oversight

 [GEA] Gene expression analysis

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [BPP] Binding of purified proteins

 [IMP] Inferred from mutant phenotype

 [SM] Site mutation

 [IEP] Inferred from expression pattern


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Bobrovskyy M., Vanderpool CK., 2016, Diverse mechanisms of post-transcriptional repression by the small RNA regulator of glucose-phosphate stress., Mol Microbiol 99(2):254-73

 [3] Durand S., Storz G., 2010, Reprogramming of anaerobic metabolism by the FnrS small RNA., Mol Microbiol 75(5):1215-31

 [4] Marincs F., Manfield IW., Stead JA., McDowall KJ., Stockley PG., 2006, Transcript analysis reveals an extended regulon and the importance of protein-protein co-operativity for the Escherichia coli methionine repressor., Biochem J 396(2):227-34

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