RegulonDB RegulonDB 10.8: Operon Form
   

rprA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: rprA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: rprA
Gene(s): rprA   Genome Browser M3D Gene expression COLOMBOS
Note(s): The expression of the gene rprA is increased when colicin (at 30 ng/ml) is added to the medium 23421615. RcsB regulates rprA transcription by both phosphorylation and acetylation. RcsBs phosphorylation and control of rprA transcription requires acetyl-phosphate (AcP) as the group donor. RcsB isolated from ackA or cob mutants is hyperacetylated, and this hyperacetylation inhibits RcsB activity through the acetylation of Lys-154, which plays an important role in rprA regulation 23852870 This is the first report that in vivo acetylation by multiple lysines of a bacterial transcription factor regulates its activity 23852870
rprA is induced under biofilm conditions 30131361
Evidence: [ICWHO] Inferred computationally without human oversight
[LTED] Length of transcript experimentally determined
Reference(s): [1] Argaman L., et al., 2001
Promoter
Name: rprAp
+1: 1770372
Distance from start of the gene: 0
Sequence: ttttaaactaatgagacgaatctgatcgacgcaaaaagtccgtatgcctactattagctcAcggttataaatcaacatatt
Evidence: [TIM]
Reference(s): [1] Argaman L., et al., 2001
TF binding sites (TFBSs)
[CpxR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd CpxR-Phosphorylated activator rprAp nd nd nd nd nd [BPP], [GEA] [3]
[RcsB,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal RcsB-Pasp56 activator rprAp 1770323 1770336 -42.5 tttaaactaaTGAGACGAATCTGAtcgacgcaaa nd [APIORCISFBSCS], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] [2]


Evidence    

 [TIM] Transcription initiation mapping

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [CV(GEA)] cross validation(GEA)

 [CV(GEA/SM)] cross validation(GEA/SM)

 [CV(SM)] cross validation(SM)

 [SM] Site mutation


Reference(s)    

 [1] Argaman L., Hershberg R., Vogel J., Bejerano G., Wagner EG., Margalit H., Altuvia S., 2001, Novel small RNA-encoding genes in the intergenic regions of Escherichia coli., Curr Biol 11(12):941-50

 [2] Majdalani N., Hernandez D., Gottesman S., 2002, Regulation and mode of action of the second small RNA activator of RpoS translation, RprA., Mol Microbiol 46(3):813-26

 [3] Vogt SL., Evans AD., Guest RL., Raivio TL., 2014, The Cpx envelope stress response regulates and is regulated by small noncoding RNAs., J Bacteriol 196(24):4229-38

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