RegulonDB RegulonDB 10.9: Operon Form
   

shiA operon and associated TUs in Escherichia coli K-12 genome


Transcription unit       
Name: shiA
Gene(s): shiA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [ICWHO] Inferred computationally without human oversight
Promoter
Name: shiAp
+1: 2053566
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 77
Sequence: ttgccgctaatcagccataaaatcatttgcagatgcgctctgtcgaggtagtctcataagGttcgtttatagatcgacggc
                           -35                   -10        +1                   
Evidence: [HTTIM]
[RS-EPT-CBR]
[TIM]
Reference(s): [1] Liu F., et al., 2018
[2] Prevost K., et al., 2007
[3] Salgado H, et al., 2012
sRNA Interaction TU
sRNA TU Regulated Function Binding Sites Regulatory Mechanism Evidence (Confirmed, Strong, Weak) Reference(s)
PosLeft PosRight Target sequence (mRNA)
small regulatory RNA RyhB shiA activator 2053566 2053616 GUUCGUUUAUAGAUCGACGGCAAUGUGAGUUACCUUUUCCAUACUAAUUAU TRANSLATION-BLOCKING, MRNA-DEGRADATION [IEP], [SM] [2]


Evidence    

 [HTTIM] High-throughput transcription initiation mapping

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [TIM] Transcription initiation mapping

 [IEP] Inferred from expression pattern

 [SM] Site mutation


Reference(s)    

 [1] Liu F., Zheng K., Chen HC., Liu ZF., 2018, Capping-RACE: a simple, accurate, and sensitive 5' RACE method for use in prokaryotes., Nucleic Acids Res 46(21):e129

 [2] Prevost K., Salvail H., Desnoyers G., Jacques JF., Phaneuf E., Masse E., 2007, The small RNA RyhB activates the translation of shiA mRNA encoding a permease of shikimate, a compound involved in siderophore synthesis., Mol Microbiol 64(5):1260-73

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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