RegulonDB RegulonDB 11.1: Operon Form
   

gpt operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: gpt
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: gpt
Gene(s): gpt   Genome Browser M3D Gene expression COLOMBOS
Evidence: [EXP-IDA-BOUNDARIES-DEFINED] Boundaries of transcription experimentally identified
[EXP-IDA-TRANSCRIPT-LEN-DETERMINATION] Length of transcript experimentally determined
Reference(s): [1] Nuesch J., et al., 1984
Promoter
Name: gptp
+1: 255914
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 63
Sequence: taaaaagacgcggactcatgtgaaatactggtttttagtgcgccagatctctataatctcGcgcaacctattttcccctcg
                         -35                        -10     +1                   
Note(s): We assigned a putative transcriptional start site to this promoter based on the observation that the majority of the promoters, whose transcriptional start sites were determined experimentally, present a distance of 6 nucleotides between the transcriptional start site and the -10 box Harley CB, Reynolds RP,1987.
Evidence: [COMP-AINF]
[COMP-HINF]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
Reference(s): [2] Huerta AM., et al., 2003
[1] Nuesch J., et al., 1984
Terminator(s)
Type: rho-independent
Sequence: ctaatcttttCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTaacttcaggc
Reference(s): [1] Nuesch J., et al., 1984


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [COMP-HINF] Inferred by a human based on computational evidence

 [COMP-HINF-POSITIONAL-IDENTIFICATION] Human inference of promoter position


Reference(s)    

 [1] Nuesch J., Schumperli D., 1984, Structural and functional organization of the gpt gene region of Escherichia coli., Gene 32(1-2):243-9

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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