RegulonDB RegulonDB 10.8: Operon Form

hemB operon and associated TUs in Escherichia coli K-12 genome

Name: hemB
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit       
Name: hemB
Gene(s): hemB   Genome Browser M3D Gene expression COLOMBOS
Note(s): A potential RNA G-quadruplex structure, formed by guanine-rich sequences located in the coding sequence region of the gene, was identified for hemB . This structure could regulate the expression of the gene, as observed for hemL gene expression 31964733.
Evidence: [BTEI] Boundaries of transcription experimentally identified
[ICWHO] Inferred computationally without human oversight
Reference(s): [1] Echelard Y., et al., 1988
Name: hemBp
+1: 389754
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 27
Sequence: tgtcaacatcgcgacaactttcgtaaaacatccctaccctgcttcaggtatactatgcccCtcgattccacaaacatcagg
                       -35                       -10        +1                   
Note(s): The -10 box of hemB (TATACT) shows a typical Pribnow box, differing only by 1 nucleotide (TATAAT). On the other hand, the -35 sequence, GTAAAA, is atypical, compared with the consensus sequence, TTGACA, for this box Echelard Y,1988. The authors suggested that mutation/deletion studies will allow better definition of its position Echelard Y,1988.
Evidence: [CV(RS-EPT-CBR)]
Reference(s): [1] Echelard Y., et al., 1988
[2] Salgado H, et al., 2012