RegulonDB RegulonDB 11.1: Operon Form
   

mprA-emrAB operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: mprA-emrAB
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: emrRAB
Gene(s): mprA, emrA, emrB   Genome Browser M3D Gene expression COLOMBOS
Note(s): Hirakawa et al. demonstrated that OmpR increases mRNA expression of the emrA gene by 6.5 times, providing possible evidence that OmpR regulates the expression of its encoding operon, emrRAB Hirakawa H,2003
The expression of the emrB gene, and probably the expression of the whole emrRAB operon, is affected by antibiotic pressure. An increase in emrB expression is maintained during exposition to increasing concentrations of tetracycline Viveiros M, Dupont M, Rodrigues L, Couto I, Davin-Regli A, Martins M, Pagès JM, Amaral L,2007
Evidence: [EXP-IMP-POLAR-MUTATION] Polar mutation
Reference(s): [1] Lomovskaya O., et al., 1995
Promoter
Name: mprAp
+1: 2810747
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 23
Sequence: gaatcatttttctaaaacaatacatttactttatttgtcactgtcgttactatatcggctGaaattaatgaggtcataccc
                         -35                    -10         +1                   
Evidence: [COMP-AINF]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
[EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
Reference(s): [2] Huerta AM., et al., 2003
[1] Lomovskaya O., et al., 1995
[3] Xiong A., et al., 2000
TF binding sites (TFBSs)
[MprA,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal MprA repressor mprAp 2810727 2810747 -10.0 ttatttgtcaCTGTCGTTACTATATCGGCTGaaattaatga nd [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] nd [3]


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: forward
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -15.5 2811348 2811377 aaaatgactgGCCAGCATCGCAACATGCTGGCCTTTTTGgcaagcaggt
  anti-terminator -13.9 2811289 2811359 gttctcgaagCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTTATAAAAGAAAAAATGACTGGCCAGCATCGCaacatgctgg
  anti-anti-terminator -12.54 2811278 2811324 aagacggtgtGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAgatttataaa
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [COMP-HINF-POSITIONAL-IDENTIFICATION] Human inference of promoter position

 [EXP-IDA-TRANSCRIPTION-INIT-MAPPING] Transcription initiation mapping

 [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins


Reference(s)    

 [1] Lomovskaya O., Lewis K., Matin A., 1995, EmrR is a negative regulator of the Escherichia coli multidrug resistance pump EmrAB., J Bacteriol 177(9):2328-34

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Xiong A., Gottman A., Park C., Baetens M., Pandza S., Matin A., 2000, The EmrR protein represses the Escherichia coli emrRAB multidrug resistance operon by directly binding to its promoter region., Antimicrob Agents Chemother 44(10):2905-7

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