RegulonDB RegulonDB 10.8: Operon Form
   

gfcA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: gfcA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: gfcA
Gene(s): gfcA   Genome Browser M3D Gene expression COLOMBOS
Note(s): A potential RNA G-quadruplex structure, formed by guanine-rich sequences located in the coding sequence region of the gene, was identified for gfcA . This structure could regulate the expression of the gene, as observed for hemL gene expression 31964733.
Evidence: [ICWHO] Inferred computationally without human oversight
Promoter
Name: gfcAp
+1: 1050702
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 958
Sequence: ttcgtaacaatttccttatctttaatttgtattttgctaagatttatcttatgctcacaaGtcgtatttccagagggggat
                           -35                    -10       +1                   
Evidence: [TIM]
Reference(s): [1] Wiebe H., et al., 2015
TF binding sites (TFBSs)
[YjjQ,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal YjjQ repressor gfcAp 1050711 1050725 -16.0 tgtattttgcTAAGATTTATCTTATgctcacaagt nd [AIBSCS], [CV(CHIP-SV/GEA/ROMA)], [CV(CHIP-SV/SM)], [CV(GEA/ROMA)], [CV(GEA/ROMA/SM)], [GEA], [SM] [1]
proximal YjjQ repressor gfcAp 1050744 1050758 -49.0 atagctttcgTAACAATTTCCTTATctttaatttg nd [AIBSCS], [CV(GEA/ROMA)], [GEA] [1]


Evidence    

 [TIM] Transcription initiation mapping

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [CV(CHIP-SV/GEA/ROMA)] cross validation(CHIP-SV/GEA/ROMA)

 [CV(CHIP-SV/SM)] cross validation(CHIP-SV/SM)

 [CV(GEA/ROMA)] cross validation(GEA/ROMA)

 [CV(GEA/ROMA/SM)] cross validation(GEA/ROMA/SM)

 [GEA] Gene expression analysis

 [SM] Site mutation


Reference(s)    

 [1] Wiebe H., Gurlebeck D., Gross J., Dreck K., Pannen D., Ewers C., Wieler LH., Schnetz K., 2015, YjjQ Represses Transcription of flhDC and Additional Loci in Escherichia coli., J Bacteriol 197(16):2713-20

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