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| Operon |  |
|---|---|
| Name: | dnaK-tpke11-dnaJ |
| This page displays every known transcription unit of this operon and their known regulation. | |
| Name: | dnaKJ |
| Gene(s): | dnaK, tpke11, dnaJ Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | The expression of the dnaKJ operon is increased under the stress condition caused by the introduction of the R1-16 plasmid, which encodes the type IV secretion system (T4S machinery) in the cell. dnaKJ operon expression is σ32 dependent, and it was observed that the expression of the σ32 gene is enhanced with the R1-16 plasmid in the cell in a CpxR-dependent way Zahrl D,2006 A potential RNA G-quadruplex structure, formed by guanine-rich sequences located in the coding sequence region of the gene, was identified for dnaK . This structure could regulate the expression of the gene, as observed for hemL gene expression 31964733. |
| Evidence: | [PM] Polar mutation |
| Reference(s): |
[1] Thomas JG., et al., 1996 [2] Zhou YN., et al., 1988 |
| Promoter | |
| Name: | dnaKp1 |
| +1: | 12048 |
| Sigma Factor: | Sigma32 Sigmulon |
| Distance from start of the gene: | 115 |
| Sequence: |
gcacaaaaaatttttgcatctcccccttgatgacgtggtttacgaccccatttagtagtcAaccgcagtgagtgagtctgc -35 -10 +1 |
| Note(s): | Transcripts from dnaKp1 and dnaKp2 are the major products of in vitro transcription of the dnaKJ operon by σ32. This sigma holoenzyme transcribes dnaKp3 poorly. |
| Evidence: |
[CV(RS-EPT-CBR)] [CV(TIM)] [HIPP] [IDA] [IEP] [RS-EPT-CBR] [TIM] |
| Reference(s): |
[3] Cowing DW., et al., 1985 [4] Nonaka G., et al., 2006 [5] Salgado H, et al., 2012 [6] Wade JT., et al., 2006 [7] Wagner MA., et al., 2009 [2] Zhou YN., et al., 1988 |
| Name: | dnaKJ |
| Gene(s): | dnaK, tpke11, dnaJ Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | The expression of the dnaKJ operon is increased under the stress condition caused by the introduction of the R1-16 plasmid, which encodes the type IV secretion system (T4S machinery) in the cell. dnaKJ operon expression is σ32 dependent, and it was observed that the expression of the σ32 gene is enhanced with the R1-16 plasmid in the cell in a CpxR-dependent way Zahrl D,2006 A potential RNA G-quadruplex structure, formed by guanine-rich sequences located in the coding sequence region of the gene, was identified for dnaK . This structure could regulate the expression of the gene, as observed for hemL gene expression 31964733. |
| Evidence: | [PM] Polar mutation |
| Reference(s): | [8] Bardwell JC., et al., 1986 |
| Promoter | |
| Name: | dnaKp2 |
| +1: | 12123 |
| Sigma Factor: | Sigma32 Sigmulon |
| Distance from start of the gene: | 40 |
| Sequence: |
gtctgcaaaaaaatgaaattgggcagttgaaaccagacgtttcgcccctattacagactcAcaaccacatgatgaccgaat -35 -10 +1 |
| Note(s): | Transcripts from dnaKp1 and dnaKp2 are the major products of in vitro transcription of the dnaKJ operon by σ32. This sigma holoenzyme transcribes dnaKp3 poorly. |
| Evidence: |
[CV(TIM)] [HIPP] [IDA] [IEP] [TIM] |
| Reference(s): |
[3] Cowing DW., et al., 1985 [4] Nonaka G., et al., 2006 [6] Wade JT., et al., 2006 [7] Wagner MA., et al., 2009 [2] Zhou YN., et al., 1988 |
| Name: | dnaKJ |
| Gene(s): | dnaK, tpke11, dnaJ Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | The expression of the dnaKJ operon is increased under the stress condition caused by the introduction of the R1-16 plasmid, which encodes the type IV secretion system (T4S machinery) in the cell. dnaKJ operon expression is σ32 dependent, and it was observed that the expression of the σ32 gene is enhanced with the R1-16 plasmid in the cell in a CpxR-dependent way Zahrl D,2006 A potential RNA G-quadruplex structure, formed by guanine-rich sequences located in the coding sequence region of the gene, was identified for dnaK . This structure could regulate the expression of the gene, as observed for hemL gene expression 31964733. |
| Evidence: | [PM] Polar mutation |
| Reference(s): | [8] Bardwell JC., et al., 1986 |
| Promoter | |
| Name: | dnaKp3 |
| +1: | 12144 |
| Sigma Factor: | Sigma32 Sigmulon |
| Distance from start of the gene: | 19 |
| Sequence: |
ggcagttgaaaccagacgtttcgcccctattacagactcacaaccacatgatgaccgaatAtatagtggagacgtttagat -35 -10 +1 |
| Note(s): | Transcripts from dnaKp1 and dnaKp2 are the major products of in vitro transcription of the dnaKJ operon by σ32, but this sigma factor polymerase transcribes dnaKp3 poorly. |
| Evidence: |
[CV(TIM)] [HIPP] [IDA] [IEP] [TIM] |
| Reference(s): |
[3] Cowing DW., et al., 1985 [6] Wade JT., et al., 2006 [7] Wagner MA., et al., 2009 [2] Zhou YN., et al., 1988 |
| Name: | tpke11 |
| Gene(s): | tpke11 Genome Browser M3D Gene expression COLOMBOS |
| Evidence: | [IHBCE] Inferred by a human based on computational evidence |
| RNA cis-regulatory element | |
|---|
| Regulation, transcriptional elongation | |
| Attenuator type: | Translational |
| Strand: | forward |
| Structure type | Energy | LeftPos | RightPos | Sequence (RNA-strand) | |
|---|---|---|---|---|---|
| terminator | -6.6 | 14134 | 14155 | ttcctctccgCCCGTGCATTCATCTAGGGGCaatttaaaaa |
| Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos" |
| Reference(s) |
|
|---|---|
[CV(RS-EPT-CBR)] cross validation(RS-EPT-CBR)