RegulonDB RegulonDB 10.8: Operon Form
   

lolB-ispE-prs operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: lolB-ispE-prs
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: prs
Synonym(s): OP00286, prsA
Gene(s): prs   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] He B., et al., 1993
Promoter
Name: prsp
+1: 1262177
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 302
Sequence: tcgcaagaaaacgttttcgcgaggttgatgcggtgctttcctggctgttagaatacgcccCgtcgcgcctgactgggacag
                         -35                     -10        +1                   
Evidence: [TIM]
Reference(s): [2] Hove-Jensen B., et al., 1986
Terminator(s)
Type: rho-independent
Sequence: agacaaaaaaACCCGCCGCAGCGGGtctttgagcc
Reference(s): [2] Hove-Jensen B., et al., 1986
TF binding sites (TFBSs)
[PurR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal PurR-hypoxanthine repressor prsp 1262218 1262233 -48.5 aggttatcgcAAGAAAACGTTTTCGCgaggttgatg nd [APIORCISFBSCS], [BCE], [CV(GEA)], [GEA] [1]


Transcription unit       
Name: lolB-ispE-prs
Synonym(s): lolB-ispE-prsA
Gene(s): prs, ispE, lolB   Genome Browser M3D Gene expression COLOMBOS
Evidence: [IEP] Inferred from expression pattern
Reference(s): [3] Post DA., et al., 1993
Promoter
Name: lolBp
+1: 1263556
Sigma Factor: Sigma28 Sigmulon
Distance from start of the gene: 56
Sequence: ttgccgaaagccgttagattctggcaattaagacaacttgaacatagacgatagcggacgGtaacgctagcattaagggtt
                            -35                -10          +1                   
Evidence: [HIPP]
Reference(s): [4] Gama-Castro S., et al., 2008
[3] Post DA., et al., 1993
Terminator(s)
Type: rho-independent
Sequence: agacaaaaaaACCCGCCGCAGCGGGtctttgagcc
Reference(s): [2] Hove-Jensen B., et al., 1986


Evidence    

 [TIM] Transcription initiation mapping

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BCE] Binding of cellular extracts

 [CV(GEA)] cross validation(GEA)

 [GEA] Gene expression analysis

 [HIPP] Human inference of promoter position


Reference(s)    

 [1] He B., Choi KY., Zalkin H., 1993, Regulation of Escherichia coli glnB, prsA, and speA by the purine repressor., J Bacteriol 175(11):3598-606

 [2] Hove-Jensen B., Harlow KW., King CJ., Switzer RL., 1986, Phosphoribosylpyrophosphate synthetase of Escherichia coli. Properties of the purified enzyme and primary structure of the prs gene., J Biol Chem 261(15):6765-71

 [3] Post DA., Hove-Jensen B., Switzer RL., 1993, Characterization of the hemA-prs region of the Escherichia coli and Salmonella typhimurium chromosomes: identification of two open reading frames and implications for prs expression., J Gen Microbiol 139(2):259-66

 [4] Gama-Castro S., Jimenez-Jacinto V., Peralta-Gil M., Santos-Zavaleta A., Penaloza-Spinola MI., Contreras-Moreira B., Segura-Salazar J., Muniz-Rascado L., Martinez-Flores I., Salgado H., Bonavides-Martinez C., Abreu-Goodger C., Rodriguez-Penagos C., Miranda-Rios J., Morett E., Merino E., Huerta AM., Trevino-Quintanilla L., Collado-Vides J., 2008, RegulonDB (version 6.0): gene regulation model of Escherichia coli K-12 beyond transcription, active (experimental) annotated promoters and Textpresso navigation., Nucleic Acids Res 36(Database issue):D120-4

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