RegulonDB RegulonDB 10.8: Operon Form
   

hemA-prfA-prmC operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: hemA-prfA-prmC
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: hemA
Gene(s): hemA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Verkamp E., et al., 1989
Promoter
Name: hemAp2
+1: 1263583
Distance from start of the gene: 131
Sequence: acgttgcagttataacccttaatgctagcgttaccgtccgctatcgtctatgttcaagttGtcttaattgccagaatctaa
Evidence: [TIM]
Reference(s): [2] Choi P., et al., 1996
[1] Verkamp E., et al., 1989


Transcription unit          
Name: hemA-prfA-prmC
Gene(s): hemA, prfA, prmC   Genome Browser M3D Gene expression COLOMBOS
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [3] Dahlgren A., et al., 2004
[1] Verkamp E., et al., 1989
Promoter
Name: hemAp1
+1: 1263676
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 38
Sequence: attactccaaaagggggcgctctcttttattgatcttacgcatcctgtatgatgcaagcaGactaaccctatcaacgttgg
                          -35                   -10         +1                   
Evidence: [TIM]
Reference(s): [2] Choi P., et al., 1996
[1] Verkamp E., et al., 1989
TF binding sites (TFBSs)
[ArcA,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd ArcA-Phosphorylated activator hemAp1 nd nd nd nd nd [GEA] [2], [4]
[FNR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal FNR repressor hemAp1 1263645 1263658 -24.5 ctctcttttaTTGATCTTACGCATcctgtatgat nd [BPP], [GEA] [2], [4], [5]
[IHF,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd IHF activator hemAp1 nd nd nd nd nd [GEA] [4]


Evidence    

 [TIM] Transcription initiation mapping

 [GEA] Gene expression analysis

 [BPP] Binding of purified proteins


Reference(s)    

 [1] Verkamp E., Chelm BK., 1989, Isolation, nucleotide sequence, and preliminary characterization of the Escherichia coli K-12 hemA gene., J Bacteriol 171(9):4728-35

 [2] Choi P., Wang L., Archer CD., Elliott T., 1996, Transcription of the glutamyl-tRNA reductase (hemA) gene in Salmonella typhimurium and Escherichia coli: role of the hemA P1 promoter and the arcA gene product., J Bacteriol 178(3):638-46

 [3] Dahlgren A., Ryden-Aulin M., 2004, Effects of two cis-acting mutations on the regulation and expression of release factor one in Escherichia coli., Biochimie 86(7):431-8

 [4] Darie S., Gunsalus RP., 1994, Effect of heme and oxygen availability on hemA gene expression in Escherichia coli: role of the fnr, arcA, and himA gene products., J Bacteriol 176(17):5270-6

 [5] Melville SB., Gunsalus RP., 1996, Isolation of an oxygen-sensitive FNR protein of Escherichia coli: interaction at activator and repressor sites of FNR-controlled genes., Proc Natl Acad Sci U S A 93(3):1226-31

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