RegulonDB RegulonDB 10.6.3: Operon Form
   

mreBCD operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: mreBCD
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: mreBCD
Gene(s): mreD, mreC, mreB   Genome Browser M3D Gene expression COLOMBOS
Note(s): Based on sequence analysis, |CITS: [1937035]| suggested that this transcription unit was shaped by five genes, mreBCD-yhdE-rng. However, |CITS:[17090951]| demonstrated through Northern blot and gene expression analyses that this operon has three genes only, mreBCD. This operon appears to be positively regulated by FlhDC |CITS:[11287152]|, but it is not known which of the three promoters transcribing the operon is affected by the protein.
Evidence: [CV(LTED/PM)] cross validation(LTED/PM)
[LTED] Length of transcript experimentally determined
[PM] Polar mutation
Reference(s): [1] Wachi M., et al., 2006
Promoter
Name: mreBp1
+1: 3401129
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 42
Sequence: ttcaccgtagcagatgatttttgcgccttgtcgctgctgcgtgtggttggtaaagtaagcGgattttcttttccgccccag
                            -10                    -35      +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [1] Wachi M., et al., 2006
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd BolA repressor mreBp1 nd nd nd nd nd [BPP], [GEA] [2]


Transcription unit          
Name: mreB
Gene(s): mreB   Genome Browser M3D Gene expression COLOMBOS
Note(s): The mreB gene appears to be positively regulated by FlhDC |CITS:[11287152]|, but it is not known which of the three promoters transcribing the gene is affected by the protein.
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Wachi M., et al., 2006
Promoter
Name: mreBp1
+1: 3401129
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 42
Sequence: ttcaccgtagcagatgatttttgcgccttgtcgctgctgcgtgtggttggtaaagtaagcGgattttcttttccgccccag
                            -10                    -35      +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [1] Wachi M., et al., 2006
Terminator(s)
Type: rho-independent
Sequence: cgtatcagacCAGGCAGGGTAAACAGACACTTCCCCTGCCTGcatccgatta
Reference(s): [1] Wachi M., et al., 2006
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd BolA repressor mreBp1 nd nd nd nd nd [BPP], [GEA] [2]


Transcription unit       
Name: mreB
Gene(s): mreB   Genome Browser M3D Gene expression COLOMBOS
Note(s): The mreB gene appears to be positively regulated by FlhDC |CITS:[11287152]|, but it is not known which of the three promoters transcribing the gene is affected by the protein.
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Wachi M., et al., 2006
Promoter
Name: mreBp2
+1: 3401193
Distance from start of the gene: 106
Sequence: gatgcattaagcctttctggactcaggcagagatttgtaacaaaggaaacgaactgcactAattttcaccgtagcagatga
Evidence: [TIM]
Reference(s): [1] Wachi M., et al., 2006
Terminator(s)
Type: rho-independent
Sequence: cgtatcagacCAGGCAGGGTAAACAGACACTTCCCCTGCCTGcatccgatta
Reference(s): [1] Wachi M., et al., 2006


Transcription unit       
Name: mreBCD
Gene(s): mreD, mreC, mreB   Genome Browser M3D Gene expression COLOMBOS
Note(s): Based on sequence analysis, |CITS: [1937035]| suggested that this transcription unit was shaped by five genes, mreBCD-yhdE-rng. However, |CITS:[17090951]| demonstrated through Northern blot and gene expression analyses that this operon has three genes only, mreBCD. This operon appears to be positively regulated by FlhDC |CITS:[11287152]|, but it is not known which of the three promoters transcribing the operon is affected by the protein.
Evidence: [CV(LTED/PM)] cross validation(LTED/PM)
[LTED] Length of transcript experimentally determined
[PM] Polar mutation
Reference(s): [1] Wachi M., et al., 2006
Promoter
Name: mreBp2
+1: 3401193
Distance from start of the gene: 106
Sequence: gatgcattaagcctttctggactcaggcagagatttgtaacaaaggaaacgaactgcactAattttcaccgtagcagatga
Evidence: [TIM]
Reference(s): [1] Wachi M., et al., 2006


Transcription unit       
Name: mreB
Gene(s): mreB   Genome Browser M3D Gene expression COLOMBOS
Note(s): The mreB gene appears to be positively regulated by FlhDC |CITS:[11287152]|, but it is not known which of the three promoters transcribing the gene is affected by the protein.
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Wachi M., et al., 2006
Promoter
Name: mreBp3
+1: 3401356
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 269
Sequence: atattcacaaagatactcggtttaacctgccgtttaatccgttttcacgtagaataatgcGcgctgcgtctcatgggagtg
                           -10                    -35       +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [1] Wachi M., et al., 2006
Terminator(s)
Type: rho-independent
Sequence: cgtatcagacCAGGCAGGGTAAACAGACACTTCCCCTGCCTGcatccgatta
Reference(s): [1] Wachi M., et al., 2006


Transcription unit       
Name: mreBCD
Gene(s): mreD, mreC, mreB   Genome Browser M3D Gene expression COLOMBOS
Note(s): Based on sequence analysis, |CITS: [1937035]| suggested that this transcription unit was shaped by five genes, mreBCD-yhdE-rng. However, |CITS:[17090951]| demonstrated through Northern blot and gene expression analyses that this operon has three genes only, mreBCD. This operon appears to be positively regulated by FlhDC |CITS:[11287152]|, but it is not known which of the three promoters transcribing the operon is affected by the protein.
Evidence: [ITCR] Inferred through co-regulation
[LTED] Length of transcript experimentally determined
Reference(s): [1] Wachi M., et al., 2006
Promoter
Name: mreBp3
+1: 3401356
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 269
Sequence: atattcacaaagatactcggtttaacctgccgtttaatccgttttcacgtagaataatgcGcgctgcgtctcatgggagtg
                           -10                    -35       +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [1] Wachi M., et al., 2006


Transcription unit       
Name: mreCD
Gene(s): mreD, mreC   Genome Browser M3D Gene expression COLOMBOS
Evidence: [PAGTSBP] Products of adjacent genes in the same biological process
Promoter
Name: mreCp
+1: Unknown
Note(s): Zaslaver et al. demonstrated in 2006, by means of a library of fluorescent transcription fusions, that this promoter can be transcribed in vitro Zaslaver A,2006. Based on this, a putative promoter was suggested, but the +1 site of the transcription initiation has not been determined, although there exists promoter activity.
Evidence: [IEP]
Reference(s): [3] Zaslaver A., et al., 2006





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