RegulonDB RegulonDB 10.7: Operon Form
   

slp-dctR operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: slp-dctR
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: slp-dctR
Synonym(s): OP00136, slpA
Gene(s): slp, dctR   Genome Browser M3D Gene expression COLOMBOS
Note(s): Microarray analyses have shown that the slp-dctR operon is activated by the transcriptional regulator GadX, but it is not known if the effect of the regulator on the operon is direct or indirect. The positive regulation of the slp-dctR operon by GadX is positively influenced by the transcriptional regulator GadW. In contrast to this, slp gene expression appears to be negatively regulated by GadW alone |CITS:[12730179]| .
The transcription of slp appears to be increased under acidic growth conditions during the exponential phase in a RcsB-dependent manner, but not during stationary phase |CITS:[21571995]|.
Indole enhances the expression of several genes related to acid resistance, such as gadA, gadB, gadC, hdeA, hdeB, hdeD, slp, and gadE |CITS:[20470880]|. The acid resistance phenotype induced by indoles is mainly due to increased expression of the glutamine decarboxylase system |CITS:[20470880]|.
Evidence: [ITC] Inferred through co-regulation
[LTED] Length of transcript experimentally determined
Reference(s): [1] Alexander DM., et al., 1994
[2] Tucker DL., et al., 2003
Promoter
Name: slpp
+1: 3653936
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 25
Sequence: tatcaggcatatcacctcagaatcagatgaaaactataaagaaatatctattatggttttAatatttgttgataaggatag
                           -35                   -10        +1                   
Evidence: [HIPP]
[RS-EPT-CBR]
[TIM] ; Method: Primer Extension
[TIM]
Reference(s): [1] Alexander DM., et al., 1994
[3] Salgado H, et al., 2012
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd GadE-RcsB activator slpp nd nd nd nd nd [BPP], [GEA] [9], [10]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal GadW repressor slpp 3653845 3653864 -81.5 ctaaacagaaAGGATATAAACATCAGACAGgtttacgtta nd [APIORCISFBSCS], [GEA] [2], [5]
proximal GadW repressor slpp 3653866 3653885 -60.5 atcagacaggTTTACGTTACTATCAGGCATatcacctcag nd [AIBSCS], [GEA] [2], [5]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal GadX activator slpp 3653845 3653864 -81.5 ctaaacagaaAGGATATAAACATCAGACAGgtttacgtta nd [APIORCISFBSCS], [BPP], , [GEA], [IHBCE], [2], [4], [5]
proximal GadX activator slpp 3653856 3653875 -70.5 ggatataaacATCAGACAGGTTTACGTTACtatcaggcat nd , [IHBCE], [4]
proximal GadX activator slpp 3653866 3653885 -60.5 atcagacaggTTTACGTTACTATCAGGCATatcacctcag nd [AIBSCS], [BPP], [GEA] [2], [5]
proximal GadX activator slpp 3653923 3653942 -3.0 aaagaaatatCTATTATGGTTTTAATATTTgttgataagg nd , [IHBCE], [4]
proximal GadX activator slpp 3653942 3653961 17.0 ttttaatattTGTTGATAAGGATAGTAACAtgaacatgac nd , [IHBCE], [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd H-NS repressor slpp nd nd nd nd nd [BPP] [11]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote MarA repressor slpp 3653964 3653983 39.0 tagtaacatgAACATGACAAAAGGTGCACTcatcctcagc nd [APIORCISFBSCS], [GEA] [7], [8]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal YdeO activator slpp 3653884 3653909 -40.0 actatcaggcATATCACCTCAGAATCAGATGAAAACtataaagaaa nd [BPP], [GEA], [IHBCE] [6]


Regulation by sRNA    
  Small RNA name (Regulator) Regulation type Mechanism Function Binding Sites Evidence Reference
LeftPos RightPos Sequence (RNA-strand)
  gcvB unknown unknown activator       [IMP] [12]
[13]
Notes: "The provided sequence is that of the RNA strand,i.e. 'U's are showed instead the 'T'"




Reference(s)    

 [1] Alexander DM., St John AC., 1994, Characterization of the carbon starvation-inducible and stationary phase-inducible gene slp encoding an outer membrane lipoprotein in Escherichia coli., Mol Microbiol 11(6):1059-71

 [2] Tucker DL., Tucker N., Ma Z., Foster JW., Miranda RL., Cohen PS., Conway T., 2003, Genes of the GadX-GadW regulon in Escherichia coli., J Bacteriol 185(10):3190-201

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [4] Seo SW., Kim D., O'Brien EJ., Szubin R., Palsson BO., 2015, Decoding genome-wide GadEWX-transcriptional regulatory networks reveals multifaceted cellular responses to acid stress in Escherichia coli., Nat Commun 6:7970

 [5] Tramonti A., De Canio M., De Biase D., 2008, GadX/GadW-dependent regulation of the Escherichia coli acid fitness island: transcriptional control at the gadY-gadW divergent promoters and identification of four novel 42 bp GadX/GadW-specific binding sites., Mol Microbiol 70(4):965-82

 [6] Yamanaka Y., Oshima T., Ishihama A., Yamamoto K., 2014, Characterization of the YdeO regulon in Escherichia coli., PLoS One 9(11):e111962

 [7] Price GP., St John AC., 2000, Purification and analysis of expression of the stationary phase-inducible slp lipoprotein in Escherichia coli: role of the Mar system., FEMS Microbiol Lett 193(1):51-6

 [8] Seoane AS., Levy SB., 1995, Identification of new genes regulated by the marRAB operon in Escherichia coli., J Bacteriol 177(3):530-5

 [9] Johnson MD., Burton NA., Gutierrez B., Painter K., Lund PA., 2011, RcsB Is Required for Inducible Acid Resistance in Escherichia coli and Acts at gadE-Dependent and -Independent Promoters., J Bacteriol 193(14):3653-6

 [10] Krin E., Danchin A., Soutourina O., 2010, RcsB plays a central role in H-NS-dependent regulation of motility and acid stress resistance in Escherichia coli., Res Microbiol 161(5):363-371

 [11] Krin E., Danchin A., Soutourina O., 2010, Decrypting the H-NS-dependent regulatory cascade of acid stress resistance in Escherichia coli., BMC Microbiol 10:273

 [12] Pulvermacher SC., Stauffer LT., Stauffer GV., 2009, Role of the sRNA GcvB in regulation of cycA in Escherichia coli., Microbiology 155(Pt 1):106-14

 [13] Tucker DL., Tucker N., Conway T., 2002, Gene expression profiling of the pH response in Escherichia coli., J Bacteriol 184(23):6551-8


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