RegulonDB RegulonDB 10.8: Operon Form

tff-rpsB-tsf operon and associated TUs in Escherichia coli K-12 genome

Name: tff-rpsB-tsf
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit       
Name: rpsB-tsf
Gene(s): tff, rpsB, tsf   Genome Browser M3D Gene expression COLOMBOS
Note(s): There is a typical rho-independent termination or attenuation site in the intergenic region of rpsB and tsf which is preceded by a very stable stem-loop structure that, in its RNA form, is reminiscent of an RNA polymerase III site An G,1981. However, neither an attenuator effect nor promoter activity was observed An G,1981.
Reference(s): [1] An G., et al., 1981
Name: rpsBp
+1: 189712
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 0
Sequence: tgctgtacacagcgccaacaattattggtgtccacgacgtatttgtggtataaagcgcgcCggacttccgatccatttcgt
                         -35                     -10        +1                   
Note(s): The rpsB promoter belongs to the extended -10 promoter class Aseev LV,2008 It comprises a TGTG extension upstream of the -10 hexamer TATAAA, a suboptimal -35 box, TTGGTG, and a GC-rich discriminator, GCGCGC, that separates the -10 element from the transcription start site and is reminiscent of a GC-rich discriminator typical for promoters under growth rate and stringent control Aseev LV,2014. Aseev LV,2008and references therein|. The design of the rpsB promoter region is highly conserved in γ-proteobacteria Aseev LV,2008
Site-directed mutation on both the TGTG extension and the TTG element in the -35 region had effects on transcription, since they dramatically decreased the activity of the promoter Aseev LV,2014
Evidence: [CV(RS-EPT-CBR)]
Reference(s): [2] Aseev LV., et al., 2008
[3] Mendoza-Vargas A., et al., 2009
[4] Salgado H, et al., 2012
Type: rho-independent
Sequence: atcaaaaaggAGCCGCCTGAGGGCGGCTtctttttgtg
Reference(s): [1] An G., et al., 1981
Allosteric regulation of RNA-polymerase
  Regulator Function Promoter target of RNApol Growth Conditions Note Evidence Reference
  ppGpp inhibition rpsBp Upon amino acid starvation, rpsBp is negatively regulated by ppGpp due to the presence of the GC-rich discriminator, whose substitution for the AT-rich element abolished stringent control Aseev LV,2014 [GEA] [5]
Evidence: [GEA] Gene expression analysis
Reference(s): [5] Aseev LV., et al., 2014

RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: forward
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -18.9 190768 190808 gtacacgtttGGTTAGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTATatctgtgcgg
  terminator -10.8 189676 189714 caacaattatTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGacttccgatc
  anti-terminator -13.6 190738 190781 tgaagggggaGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGCCTgcatatggcc
  anti-anti-terminator -7.62 190710 190760 gaagataacgCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTacacgtttgg
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"


 [1] An G., Bendiak DS., Mamelak LA., Friesen JD., 1981, Organization and nucleotide sequence of a new ribosomal operon in Escherichia coli containing the genes for ribosomal protein S2 and elongation factor Ts., Nucleic Acids Res 9(16):4163-72

 [2] Aseev LV., Levandovskaya AA., Tchufistova LS., Scaptsova NV., Boni IV., 2008, A new regulatory circuit in ribosomal protein operons: S2-mediated control of the rpsB-tsf expression in vivo., RNA 14(9):1882-94

 [3] Mendoza-Vargas A., Olvera L., Olvera M., Grande R., Vega-Alvarado L., Taboada B., Jimenez-Jacinto V., Salgado H., Juarez K., Contreras-Moreira B., Huerta AM., Collado-Vides J., Morett E., 2009, Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli., PLoS One 4(10):e7526

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [5] Aseev LV., Koledinskaya LS., Boni IV., 2014, Dissecting the extended "-10" Escherichia coli rpsB promoter activity and regulation in vivo., Biochemistry (Mosc) 79(8):776-84