RegulonDB RegulonDB 10.7: Operon Form
   

hicAB operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: hicAB
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Gene(s): hicA, hicB   Genome Browser M3D Gene expression COLOMBOS
Evidence: [ICWHO] Inferred computationally without human oversight
[PAGTSBP] Products of adjacent genes in the same biological process
Reference(s): [1] Jorgensen MG., et al., 2009
Promoter
Name: hicAp1
+1: 1509221
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 65
Sequence: cttccttcctgattttgcattgcattctgccgttgcggcgatttagtgctattttcgagcAaattacacacggaggtaaac
                           -35                    -10       +1                   
Evidence: [TIM] ; Method: Primer Extension
[TIM]
Reference(s): [1] Jorgensen MG., et al., 2009
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP1 activator hicAp1 1509152 1509174 -58.5 cgcaaagaaaTTCTGGAATCTTCCTTCCTGATTttgcattgca nd [APIORCISFBSCS], [GEA] [2]
Note(s): 1The gene expression of hicA was analyzed in a mutant of Sxy, a protein that is known to be a coregulator of CRP. The CRP effect on hicA expression was inferred from the results of gene expression analysis using the Sxy mutant Turnbull KJ,2017.1The gene expression of hicA was analyzed in a mutant of Sxy, a protein that is known to be a coregulator of CRP. The CRP effect on hicA expression was inferred from the results of gene expression analysis using the Sxy mutant Turnbull KJ,2017.


Transcription unit          
Name: hicAB
Gene(s): hicA, hicB   Genome Browser M3D Gene expression COLOMBOS
Note(s): When hicAB mRNA is produced from hicAp2, only the hicB cistron is translated; that is, this mRNA produces only HicB but not the HicA protein. This happens because this transcript starts just 1 base upstream of the SD sequence of hicA |CITS: [28266056]|.
Evidence: [ICWHO] Inferred computationally without human oversight
[PAGTSBP] Products of adjacent genes in the same biological process
Promoter
Name: hicAp2
+1: 1509275
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 11
Sequence: tcgagcaaattacacacggaggtaaacactaatgtttactttgttgatatactcagcggcAgggaggcgatgtgaaacaaa
                       -35                      -10         +1                   
Note(s): The activation of the promoter hicAp2 is not affected by the Sxy protein, whereas the hicAp1 promoter is Turnbull KJ,2017.
Evidence: [HIPP]
[TIM]
Reference(s): [2] Turnbull KJ., et al., 2017
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal HicB1 repressor hicAp2 1509234 1509255 -30.5 ttacacacggAGGTAAACACTAATGTTTACTTtgttgatata nd [BPP], [GEA], [SM] [2]
Note(s): 1When HicA is in excess in the cell, it appears to destabilize the binding of HicB to DNA, causing the derepression of hicAB transcription Turnbull KJ,2017.1When HicA is in excess in the cell, it appears to destabilize the binding of HicB to DNA, causing the derepression of hicAB transcription Turnbull KJ,2017.





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