RegulonDB RegulonDB 11.0: Operon Form
   

murR operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: murR
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: murR
Gene(s): murR   Genome Browser M3D Gene expression COLOMBOS
Note(s): murR and murQ are divergently orientated and expressed from nonoverlapping face-to-face (convergent) promoters, and their transcripts are complementary at the 5' end. CRP controls positively murQP expression and it also may affect murR transcription as a consequence of this unusual promoter arrangement (face-to-face) Jaeger T,2008
CRP and MurR both repress murR expression beyond transcription initiation, possibly by generating a roadblock for RNA polymerase Jaeger T,2008
Autoinduction of murR is carried out by an activated form of MurR, which may be an activator form after interaction with an as yet unidentified effector, possibly a IIBC component, since most phosphorylated sugars can trigger activation of murR in a MurR-dependent manner Ishii D,2009
Evidence: [ICWHO] Inferred computationally without human oversight
Promoter
Name: murRp2
+1: 2545793
Sigma Factor: Sigma38 Sigmulon
Distance from start of the gene: 184
Sequence: gcacatttccagcgtcgatacgcggtcaatttcagccgaggcggtgttcgagccttcagtAatcatcttttcaaattgcat
                             -35             -10            +1                   
Evidence: [ICWHO]
[IHBCE]
[TIM]
Reference(s): [1] Huerta AM., et al., 2003
[2] Jaeger T., et al., 2008
[3] Maciag A., et al., 2011
TF binding sites (TFBSs)
[MurR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote MurR activator murRp2 2545911 2545925 -125.0 taacatcgatCGCCGCGGCGATATCcggcagtacg nd [GEA], [APIORCISFBSCS] [4]
[MurR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote MurR repressor murRp2 2545911 2545925 -125.0 taacatcgatCGCCGCGGCGATATCcggcagtacg nd [GEA], [APIORCISFBSCS] [4]


Transcription unit          
Name: murR
Gene(s): murR   Genome Browser M3D Gene expression COLOMBOS
Note(s): murR and murQ are divergently orientated and expressed from nonoverlapping face-to-face (convergent) promoters, and their transcripts are complementary at the 5' end. CRP controls positively murQP expression and it also may affect murR transcription as a consequence of this unusual promoter arrangement (face-to-face) Jaeger T,2008
CRP and MurR both repress murR expression beyond transcription initiation, possibly by generating a roadblock for RNA polymerase Jaeger T,2008
Autoinduction of murR is carried out by an activated form of MurR, which may be an activator form after interaction with an as yet unidentified effector, possibly a IIBC component, since most phosphorylated sugars can trigger activation of murR in a MurR-dependent manner Ishii D,2009
Evidence: [ICWHO] Inferred computationally without human oversight
Promoter
Name: murRp
+1: 2545793
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 184
Sequence: gcacatttccagcgtcgatacgcggtcaatttcagccgaggcggtgttcgagccttcagtAatcatcttttcaaattgcat
                             -35                  -10       +1                   
Evidence: [ICWHO]
[IHBCE]
[TIM]
Reference(s): [1] Huerta AM., et al., 2003
[2] Jaeger T., et al., 2008
[3] Maciag A., et al., 2011
TF binding sites (TFBSs)
[MurR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote MurR activator murRp 2545911 2545925 -125.0 taacatcgatCGCCGCGGCGATATCcggcagtacg nd [GEA], [APIORCISFBSCS] [4]
[MurR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote MurR repressor murRp 2545911 2545925 -125.0 taacatcgatCGCCGCGGCGATATCcggcagtacg nd [GEA], [APIORCISFBSCS] [4]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [IHBCE] Inferred by a human based on computational evidence

 [TIM] Transcription initiation mapping

 [GEA] Gene expression analysis

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Jaeger T., Mayer C., 2008, The transcriptional factors MurR and catabolite activator protein regulate N-acetylmuramic acid catabolism in Escherichia coli., J Bacteriol 190(20):6598-608

 [3] Maciag A., Peano C., Pietrelli A., Egli T., De Bellis G., Landini P., 2011, In vitro transcription profiling of the σS subunit of bacterial RNA polymerase: re-definition of the σS regulon and identification of σS-specific promoter sequence elements., Nucleic Acids Res 39(13):5338-55

 [4] Ishii D., Ishihama A., Yamamoto K., 2009, Two modes of autoregulation of the murR repressor in Escherichia coli., Biosci Biotechnol Biochem 73(11):2528-30

RegulonDB