RegulonDB RegulonDB 10.8: Operon Form
   

dosCP operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: dosCP
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: dosCP
Synonym(s): yddV-dos
Gene(s): dosP, dosC   Genome Browser M3D Gene expression COLOMBOS
Note(s): The dosCP operon is highly expressed at low growth temperatures and in the stationary phase, and it plays an important role in the expression of curli-encoding genes in aerobically growing cells 20553324.
The products of the dosCP operon, diguanylate cyclase (DGC) and c-di-GMP phosphodiesterase, heme-regulated (c-PDE), act as an oxygen sensor protein complex and regulate the transcription of the csgBAC operon in response to oxygen availability 20553324.
The dosCP operon is the most highly expressed among σS-dependent genes encoding enzymes related to c-di-GMP metabolism Weber H,2006. Sommerfeldt N,2009.
Evidence: [ITC] Inferred through co-regulation
[LTED] Length of transcript experimentally determined
Reference(s): [1] Jonas K., et al., 2008
[2] Mendez-Ortiz MM., et al., 2006
Terminator(s)
Type: rho-independent
Sequence: aaatcacagtATTTAAGTGACAGTGTCACGTTAAATgaaaacccgc
Reference(s): [3] Lessard IA., et al., 1998


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: reverse
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -6.9 1567012 1567043 attgccgttgCGCATGCTCATTATCTGAGTATCGAGTTTTAtcgaattgtc
  anti-terminator -11.1 1567038 1567071 tccgcccattCGTGCTAAAGCCGCGGAAATTGCCGTTGCGCATgctcattatc
  anti-anti-terminator -7.6 1567073 1567102 atgagtggacCGGACTTGTCGAACAGGCAGATCCGCCCAttcgtgctaa
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"


Reference(s)    

 [1] Jonas K., Edwards AN., Simm R., Romeo T., Romling U., Melefors O., 2008, The RNA binding protein CsrA controls cyclic di-GMP metabolism by directly regulating the expression of GGDEF proteins., Mol Microbiol 70(1):236-57

 [2] Mendez-Ortiz MM., Hyodo M., Hayakawa Y., Membrillo-Hernandez J., 2006, Genome-wide transcriptional profile of Escherichia coli in response to high levels of the second messenger 3',5'-cyclic diguanylic acid., J Biol Chem 281(12):8090-9

 [3] Lessard IA., Pratt SD., McCafferty DG., Bussiere DE., Hutchins C., Wanner BL., Katz L., Walsh CT., 1998, Homologs of the vancomycin resistance D-Ala-D-Ala dipeptidase VanX in Streptomyces toyocaensis, Escherichia coli and Synechocystis: attributes of catalytic efficiency, stereoselectivity and regulation with implications for function., Chem Biol 5(9):489-504

RegulonDB