RegulonDB RegulonDB 10.10: Operon Form
   

mqsRA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: mqsRA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: mqsA
Gene(s): mqsA   Genome Browser M3D Gene expression COLOMBOS
Promoter
Name: mqsAp1
+1: 3168286
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 40
Sequence: tggcaggatgtttacagacccaggcttgttacaggccaggtttatcttaaaattacggtaAttcatgacgtactgatcgtc
                          -35                   -10         +1                   
Note(s): The activity of the promoters transcribing the mqsA gene are not affected by amino acid starvation or oxidative stress Fraikin N,2019.
Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.853. P-value: 1.177e-5
Evidence: [ICWHO]
[IEP]
[IHBCE]
[TIM]
Reference(s): [1] Fraikin N., et al., 2019
[2] Huerta AM., et al., 2003
[3] Thomason MK., et al., 2015


Transcription unit       
Name: mqsA
Gene(s): mqsA   Genome Browser M3D Gene expression COLOMBOS
Promoter
Name: mqsAp2
+1: 3168397
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 151
Sequence: ttaaatgcagatgagttaggtttggattttgatggtatgtgtaatgttatcattggattaTcagagagcgacttttataaa
                           -35                  -10         +1                   
Note(s): The activity of the promoters transcribing the mqsA gene are not affected by amino acid starvation or oxidative stress Fraikin N,2019.
Evidence: [HIPP]
[IEP]
[IHBCE]
[TIM]
Reference(s): [1] Fraikin N., et al., 2019
[3] Thomason MK., et al., 2015


Transcription unit       
Name: mqsRA
Gene(s): mqsA, mqsR   Genome Browser M3D Gene expression COLOMBOS
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [4] Kasari V., et al., 2010
[5] Yamaguchi Y., et al., 2009
Promoter
Name: mqsRp2
+1: 3168562
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 18
Sequence: aactaaccttttaggtgctttttcattgctgtaattaaccttttaggttataactaaagtAacagggaggcgggggttatg
                          -35                    -10        +1                   
Note(s): The activity of the promoters transcribing the mqsA gene are not affected by amino acid starvation or oxidative stress Fraikin N,2019.
Evidence: [ICWHO]
[IHBCE]
[TIM]
Reference(s): [6] Christensen-Dalsgaard M., et al., 2010
[2] Huerta AM., et al., 2003
[4] Kasari V., et al., 2010


Transcription unit          
Name: mqsRA
Gene(s): mqsA, mqsR   Genome Browser M3D Gene expression COLOMBOS
Note(s): Transcription of mqsR was found to be induced upon biofilm formation Ren D,2004, and deletion of mqsR decreases biofilm formation and abolishes motility Gonzalez Barrios AF,2006. mqsR is more highly expressed in persister cells than nonpersisters, and overexpression of mqsR leads to growth arrest and increased tolerance to certain antibiotics Shah D, Zhang Z, Khodursky A, Kaldalu N, Kurg K, Lewis K,2006. Zhang XS,2008. In addition, the toxins Hha, HokA, and CspD, the outer membrane proteins OppA and YbfM, and small RNA regulator Hfq are involved during persister cell formation via MqsR overexpression Kim Y, Wood TK,2010 Coinduction of mqsA expression rescues cell growth Yamaguchi Y,2009. Christensen-Dalsgaard M,2010. However, the opposite effect has also been reported Zhang XS,2008.
Evidence: [ICWHO] Inferred computationally without human oversight
[LTED] Length of transcript experimentally determined
Reference(s): [5] Yamaguchi Y., et al., 2009
Promoter
Name: mqsRp1
+1: 3168653
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 109
Sequence: caaactggcgctactaatgcgccactttcttatatggtcaatcactttccttaaagaataCgttttgtgtggtcactatct
                           -35                        -10   +1                   
Note(s): The activity of the promoters transcribing the mqsA gene are not affected by amino acid starvation or oxidative stress Fraikin N,2019.
Evidence: [ICWHO]
[TIM]
Reference(s): [2] Huerta AM., et al., 2003
[5] Yamaguchi Y., et al., 2009
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote MqsA repressor mqsRp1 3168573 3168587 74.0 ttgctgtaatTAACCTTTTAGGTTAtaactaaagt nd [GEA], [APIORCISFBSCS], [BPP], [IHBCE] [1], [5]
remote MqsA repressor mqsRp1 3168605 3168619 42.0 tacatctaacTAACCTTTTAGGTGCtttttcattg nd [GEA], [APIORCISFBSCS], [BPP], [IHBCE] [1], [5]


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: reverse
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -10.2 3168683 3168708 tgcttcaaacTGGCGCTACTAATGCGCCACTTTCTtatatggtca
  anti-terminator -5.17 3168704 3168748 gttggcgtgaTGCCTGACTCCAGCTTCCCTTATAAATTCCTGCTTCAAACTGGCgctactaatg
  anti-anti-terminator -5.8 3168735 3168758 tttatgtgccGTTGGCGTGATGCCTGACTCCAGcttcccttat
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"





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