RegulonDB RegulonDB 10.7: Operon Form
   

mdfA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: mdfA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: cmr
Gene(s): mdfA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
[LTED] Length of transcript experimentally determined
[LTED] Length of transcript experimentally determined
Reference(s): [1] Nilsen IW., et al., 1996
Promoter
Name: cmrp2
+1: 883569
Distance from start of the gene: 104
Sequence: gggtgattgtaacgggcataggttaaataaaacttaaagaaagcgtagctatactcgtaaTaatgtaagaatgtgcttaac
Evidence: [TIM]
Reference(s): [2] Eguchi Y., et al., 2003
Terminator(s)
Type: rho-independent
Sequence: gggtaaaaaaATGCCTGACTGCTTTGTGCGATCAGGCATtctcgaatta
Note(s): The putative rho-independent terminator of cmr is predicted to be closed by two A-T base pairings instead and lacks a 3' poly(U) tail in the cmr transcript.
Reference(s): [1] Nilsen IW., et al., 1996


Transcription unit       
Name: cmr
Synonym(s): mdfA
Gene(s): mdfA   Genome Browser M3D Gene expression COLOMBOS
Note(s): The expression of the cmr gene is affected by antibiotic pressure. An increase in cmr expression is maintained during exposition to increasing concentrations of tetracycline |CITS:[17426813]|.
Evidence: [BTEI] Boundaries of transcription experimentally identified
[LTED] Length of transcript experimentally determined
[LTED] Length of transcript experimentally determined
Reference(s): [1] Nilsen IW., et al., 1996
Promoter
Name: cmrp
+1: 883649
Distance from start of the gene: 24
Sequence: ccgtggtttcagctacaaaattcgctttctcgttagctgcgcttttattaaactctgcgcGattattattggcgaagaaat
Evidence: [TIM] ; Method: Primer Extension
[TIM]
Reference(s): [1] Nilsen IW., et al., 1996
Terminator(s)
Type: rho-independent
Sequence: gggtaaaaaaATGCCTGACTGCTTTGTGCGATCAGGCATtctcgaatta
Note(s): The putative rho-independent terminator of cmr is predicted to be closed by two A-T base pairings instead and lacks a 3' poly(U) tail in the cmr transcript.
Reference(s): [1] Nilsen IW., et al., 1996


Evidence    

 [TIM] Transcription initiation mapping


Reference(s)    

 [1] Nilsen IW., Bakke I., Vader A., Olsvik O., El-Gewely MR., 1996, Isolation of cmr, a novel Escherichia coli chloramphenicol resistance gene encoding a putative efflux pump., J Bacteriol 178(11):3188-93

 [2] Eguchi Y., Oshima T., Mori H., Aono R., Yamamoto K., Ishihama A., Utsumi R., 2003, Transcriptional regulation of drug efflux genes by EvgAS, a two-component system in Escherichia coli., Microbiology 149(Pt 10):2819-28

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