RegulonDB RegulonDB 10.7: Operon Form
   

yadV-htrE operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: yadV-htrE
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: yadV-htrE
Synonym(s): ecpD, htrE
Gene(s): htrE, yadV   Genome Browser M3D Gene expression COLOMBOS
Note(s): This operon was identified by complementation experiments (polarity). By sequencing from the Tn5 insertion site in the htrE gene, it was demonstrated that the htrE gene is encoded by the second ORF.
Evidence: [PM] Polar mutation
Reference(s): [1] Raina S., et al., 1993
Promoter
Name: yadVp3
+1: 156214
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 13
Sequence: atgaatatacagggaataataatttctattttatattattccctgttttaattaactctaTcagggatggtttatgttttt
                       -35                       -10        +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [1] Raina S., et al., 1993
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd IHF1 activator yadVp3 nd nd nd nd nd [GEA] [1]
Note(s): 1Isolated and studied mRNA from isogenic himA+ and himA::Tn10 (SR69 and SR1021) strains or from an himD mutant bacterial strain (SR1022). The primer extension results showed comparatively low levels of mRNA transcripts initiating from all three putative promoters. Thus, it is clear that functional IHF is needed for efficient transcription of the ecp-htrE operon.1Isolated and studied mRNA from isogenic himA+ and himA::Tn10 (SR69 and SR1021) strains or from an himD mutant bacterial strain (SR1022). The primer extension results showed comparatively low levels of mRNA transcripts initiating from all three putative promoters. Thus, it is clear that functional IHF is needed for efficient transcription of the ecp-htrE operon.


Transcription unit          
Name: yadV-htrE
Synonym(s): ecpD, htrE
Gene(s): htrE, yadV   Genome Browser M3D Gene expression COLOMBOS
Note(s): This operon was identified by complementation experiments (polarity). By sequencing from the Tn5 insertion site in the htrE gene, it was demonstrated that the htrE gene is encoded by the second ORF.
Evidence: [PM] Polar mutation
Reference(s): [1] Raina S., et al., 1993
Promoter
Name: yadVp2
+1: 156224
Sigma Factor: Sigma38 Sigmulon
Distance from start of the gene: 23
Sequence: aattgattgaatgaatatacagggaataataatttctattttatattattccctgttttaAttaactctatcagggatggt
                      -35                 -10               +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [1] Raina S., et al., 1993
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd IHF1 activator yadVp2 nd nd nd nd nd [GEA] [1]
Note(s): 1Isolated mRNA from isogenic himA+ and himA::Tn10 (SR69 and SR1021) strains or from himD mutant bacteria (SR1022) has been studied. The primer extension results showed comparatively low levels of mRNA transcripts initiating from all three putative promoters. Thus, it is clear that functional IHF is needed for efficient transcription of the ecp-htrE operon.1Isolated mRNA from isogenic himA+ and himA::Tn10 (SR69 and SR1021) strains or from himD mutant bacteria (SR1022) has been studied. The primer extension results showed comparatively low levels of mRNA transcripts initiating from all three putative promoters. Thus, it is clear that functional IHF is needed for efficient transcription of the ecp-htrE operon.


Transcription unit          
Name: yadV-htrE
Synonym(s): ecpD, htrE
Gene(s): htrE, yadV   Genome Browser M3D Gene expression COLOMBOS
Note(s): This operon was identified by complementation experiments (polarity). By sequencing from the Tn5 insertion site in the htrE gene, it was demonstrated that the htrE gene is encoded by the second ORF.
Evidence: [PM] Polar mutation
Reference(s): [1] Raina S., et al., 1993
Promoter
Name: yadVp1
+1: 156235
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 34
Sequence: ttcaccagttaaattgattgaatgaatatacagggaataataatttctattttatattatTccctgttttaattaactcta
                            -35                        -10  +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [1] Raina S., et al., 1993
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd IHF1 activator yadVp1 nd nd nd nd nd [GEA] [1]
Note(s): 1mRNA from isogenic himA+ and himA::Tn10 (SR69 and SR1021) strains or from himD mutant bacteria (SR1022) was isolated and studied. The primer extension results showed comparatively low levels of mRNA transcripts initiating from all three putative promoters. Thus, it is clear that functional IHF is needed for efficient transcription of the ecp-htrE operon.1mRNA from isogenic himA+ and himA::Tn10 (SR69 and SR1021) strains or from himD mutant bacteria (SR1022) was isolated and studied. The primer extension results showed comparatively low levels of mRNA transcripts initiating from all three putative promoters. Thus, it is clear that functional IHF is needed for efficient transcription of the ecp-htrE operon.





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