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|
| Operon |  |
|---|---|
| Name: | yadV-htrE |
| This page displays every known transcription unit of this operon and their known regulation. | |
| Name: | yadV-htrE |
| Synonym(s): | ecpD, htrE |
| Gene(s): | htrE, yadV Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | This operon was identified by complementation experiments (polarity). By sequencing from the Tn5 insertion site in the htrE gene, it was demonstrated that the htrE gene is encoded by the second ORF. |
| Evidence: | [PM] Polar mutation |
| Reference(s): | [1] Raina S., et al., 1993 |
| Promoter | |
| Name: | yadVp3 |
| +1: | 156214 |
| Sigma Factor: | Sigma70 Sigmulon |
| Distance from start of the gene: | 13 |
| Sequence: |
atgaatatacagggaataataatttctattttatattattccctgttttaattaactctaTcagggatggtttatgttttt -35 -10 +1 |
| Evidence: |
[HIPP] [TIM] |
| Reference(s): | [1] Raina S., et al., 1993 |
| Note(s): | 1Isolated and studied mRNA from isogenic himA+ and himA::Tn10 (SR69 and SR1021) strains or from an himD mutant bacterial strain (SR1022). The primer extension results showed comparatively low levels of mRNA transcripts initiating from all three putative promoters. Thus, it is clear that functional IHF is needed for efficient transcription of the ecp-htrE operon.1Isolated and studied mRNA from isogenic himA+ and himA::Tn10 (SR69 and SR1021) strains or from an himD mutant bacterial strain (SR1022). The primer extension results showed comparatively low levels of mRNA transcripts initiating from all three putative promoters. Thus, it is clear that functional IHF is needed for efficient transcription of the ecp-htrE operon. |
| Name: | yadV-htrE |
| Synonym(s): | ecpD, htrE |
| Gene(s): | htrE, yadV Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | This operon was identified by complementation experiments (polarity). By sequencing from the Tn5 insertion site in the htrE gene, it was demonstrated that the htrE gene is encoded by the second ORF. |
| Evidence: | [PM] Polar mutation |
| Reference(s): | [1] Raina S., et al., 1993 |
| Promoter | |
| Name: | yadVp2 |
| +1: | 156224 |
| Sigma Factor: | Sigma38 Sigmulon |
| Distance from start of the gene: | 23 |
| Sequence: |
aattgattgaatgaatatacagggaataataatttctattttatattattccctgttttaAttaactctatcagggatggt -35 -10 +1 |
| Evidence: |
[HIPP] [TIM] |
| Reference(s): | [1] Raina S., et al., 1993 |
| Note(s): | 1Isolated mRNA from isogenic himA+ and himA::Tn10 (SR69 and SR1021) strains or from himD mutant bacteria (SR1022) has been studied. The primer extension results showed comparatively low levels of mRNA transcripts initiating from all three putative promoters. Thus, it is clear that functional IHF is needed for efficient transcription of the ecp-htrE operon.1Isolated mRNA from isogenic himA+ and himA::Tn10 (SR69 and SR1021) strains or from himD mutant bacteria (SR1022) has been studied. The primer extension results showed comparatively low levels of mRNA transcripts initiating from all three putative promoters. Thus, it is clear that functional IHF is needed for efficient transcription of the ecp-htrE operon. |
| Name: | yadV-htrE |
| Synonym(s): | ecpD, htrE |
| Gene(s): | htrE, yadV Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | This operon was identified by complementation experiments (polarity). By sequencing from the Tn5 insertion site in the htrE gene, it was demonstrated that the htrE gene is encoded by the second ORF. |
| Evidence: | [PM] Polar mutation |
| Reference(s): | [1] Raina S., et al., 1993 |
| Promoter | |
| Name: | yadVp1 |
| +1: | 156235 |
| Sigma Factor: | Sigma70 Sigmulon |
| Distance from start of the gene: | 34 |
| Sequence: |
ttcaccagttaaattgattgaatgaatatacagggaataataatttctattttatattatTccctgttttaattaactcta -35 -10 +1 |
| Evidence: |
[HIPP] [TIM] |
| Reference(s): | [1] Raina S., et al., 1993 |
| Note(s): | 1mRNA from isogenic himA+ and himA::Tn10 (SR69 and SR1021) strains or from himD mutant bacteria (SR1022) was isolated and studied. The primer extension results showed comparatively low levels of mRNA transcripts initiating from all three putative promoters. Thus, it is clear that functional IHF is needed for efficient transcription of the ecp-htrE operon.1mRNA from isogenic himA+ and himA::Tn10 (SR69 and SR1021) strains or from himD mutant bacteria (SR1022) was isolated and studied. The primer extension results showed comparatively low levels of mRNA transcripts initiating from all three putative promoters. Thus, it is clear that functional IHF is needed for efficient transcription of the ecp-htrE operon. |
[HIPP] Human inference of promoter position