RegulonDB RegulonDB 10.7: Operon Form
   

mlc-ynfK operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: mlc-ynfK
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: mlc-ynfK
Gene(s): ynfK, mlc   Genome Browser M3D Gene expression COLOMBOS
Note(s): mlc expression is enhanced under anaerobic conditions |CITS:[11535784]|.
The expression of the gene mlc is increased under acidic growth conditions in either aerobiosis or microaerobiosis |CITS:[23274360]|. The increased expression under aerobiosis appears to be caused by the transcription factor PhoB |CITS:[23274360]|, but it is not known which promoter, of two transcribing mlc, is affected by PhoB.
Evidence: [ICWHO] Inferred computationally without human oversight
[ITC] Inferred through co-regulation
Reference(s): [1] Nonaka G., et al., 2006
Promoter
Name: mlcp2
+1: 1668591
Sigma Factor: Sigma70, Sigma32
Distance from start of the gene: 27
Sequence: cctaagtaaaaatttgacgacacgtattgaagtgcttcaccatagcctacagattatttcGgagcgcgaaaatatagggag
                           -35                 -10          +1                   
Evidence: [AIPP]
[TIM]
Reference(s): [2] Decker K., et al., 1998
[1] Nonaka G., et al., 2006
[3] Shin D., et al., 2001
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP1 activator mlcp2 1668651 1668673 -70.5 aaaagcccgaAAAATGTGCTGTTAATCACATGCctaagtaaaa nd [AIBSCS], [APIORCISFBSCS], [GEA] [3], [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Mlc repressor mlcp2 1668577 1668599 4.0 tagcctacagATTATTTCGGAGCGCGAAAATATagggagtatg nd [APIORCISFBSCS], [BCE], [GEA] [2], [3], [5]
Note(s): 1CRP has a positive effect on dgsAp2 transcription directed by σ70. However, CRP has little effect on dgsAp2 transcription directed by σ32.1CRP has a positive effect on dgsAp2 transcription directed by σ70. However, CRP has little effect on dgsAp2 transcription directed by σ32.


Transcription unit          
Name: mlc
Gene(s): mlc   Genome Browser M3D Gene expression COLOMBOS
Note(s): mlc expression is enhanced under anaerobic conditions |CITS:[11535784]|.
Under nitrogen-rich growth conditions, the expression of the dgsA gene was decreased in mutants for two genes that encode two terminal oxidases, cyoA and cydB, and in mutants for two transcriptional regulators, Fnr and Fur. However, it is unknown if the effects of the transcriptional regulators act directly on the gene expression ; also, it is unknown which of the two promoters that transcribe the gene could be regulated by the regulators |CITS:[21272324]|.
The expression of the gene mlc is increased under acidic growth conditions in either aerobiosis or microaerobiosis |CITS:[23274360]|. The increased expression under aerobiosis appears to be caused by the transcription factor PhoB |CITS:[23274360]|, but it is not known which promoter, of two transcribing mlc, is affected by PhoB.
Evidence: [ICWHO] Inferred computationally without human oversight
Promoter
Name: mlcp1
+1: 1668603
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 39
Sequence: gttaatcacatgcctaagtaaaaatttgacgacacgtattgaagtgcttcaccatagcctAcagattatttcggagcgcga
                              -35                      -10  +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [6] Cho S., et al., 2005
[2] Decker K., et al., 1998
[3] Shin D., et al., 2001
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP repressor mlcp1 1668651 1668673 -58.5 aaaagcccgaAAAATGTGCTGTTAATCACATGCctaagtaaaa nd [AIBSCS], [APIORCISFBSCS], [GEA] [3], [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Mlc repressor mlcp1 1668577 1668599 16.0 tagcctacagATTATTTCGGAGCGCGAAAATATagggagtatg nd [APIORCISFBSCS], [BCE], [GEA] [2], [3], [5]


Transcription unit       
Gene(s): ynfK   Genome Browser M3D Gene expression COLOMBOS
Evidence: [ICWHO] Inferred computationally without human oversight





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