| Name: |
rybA-mntS |
| Gene(s): |
rybA, mntS
Genome Browser
M3D Gene expression
COLOMBOS
|
| Note(s): |
RybA is a bifunctional RNA that acts as both an sRNA and an mRNA for MntS Gerstle K,2012. Waters LS,2011 Therefore, rybA and mntS overlap. Based on 5´- and 3´-RACE analysis, RybA is rapidly processed at the 5´ end as well as at multiple sites at the 3´ end. The predominant form under peroxide stress conditions is 207 nt long (positions 852270 to 852064) Gerstle K,2012 |
| Evidence: |
[BTEI] Boundaries of transcription experimentally identified
[LTED] Length of transcript experimentally determined |
| Reference(s): |
[1] Gerstle K., et al., 2012
[2] Waters LS., et al., 2011
|
| Promoter |
|
| Name: |
rybAp |
| +1: |
853064 |
| Sigma Factor: |
Sigma70 Sigmulon |
| Distance from start of the gene: |
67
|
| Sequence: |
aaccagagttgcgaaggtacaaaaaattaacgttttagcaatagctatataatatagcctGtgctatatctgtatgtaatg
-35 -10 +1
|
| Note(s): |
Based on 5´-RACE experiments, an additional 5´ end located 17 bp downstream of the transcription start site (position 852270) has been identified Waters LS,2011 Based on a deletion analysis and mutational analysis of the promoter, as well as gene expression analysis, in which the rybA transcript was expressed under the control of a constitutive lac promoter, it was proposed that this 5´ end is generated by RNA processing Gerstle K,2012 The RNase responsible for the processing is not known. |
| Evidence: |
[TIM]
|
| Reference(s): |
[1] Gerstle K., et al., 2012
|
[TIM] Transcription initiation mapping