RegulonDB RegulonDB 10.10: Operon Form
   

bluF operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: bluF
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: bluF
Gene(s): bluF   Genome Browser M3D Gene expression COLOMBOS
Note(s): Base on a σS-deficient rpoS mutant background, ycgF is under the significant negative control of rpoS Sommerfeldt N,2009 This negative control is most likely due to increased formation of a vegetative or other alternative RNAP holoenzyme in the absence of competition of σS with the other sigma factors Sommerfeldt N,2009 This suggests that σS might control ycgF
Evidence: [ICWHO] Inferred computationally without human oversight
Promoter
Name: bluFp
+1: 1215506
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 31
Sequence: aaacggttatgttttggtattaaaagaaagacacaagaagatatgtctgttaccgtcttaCtctcgcctcacccattaccc
                            -35                    -10      +1                   
Evidence: [IHBCE]
[TIM]
Reference(s): [1] Tschowri N., et al., 2012
TF binding sites (TFBSs)
[RcdA,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote RcdA activator bluFp 1215713 1215723 -211.5 ttgtacgaaaTATGTGTACAGaatgataaat nd [GEA], [APIORCISFBSCS], [BPP] [2]
remote RcdA activator bluFp 1215724 1215734 -222.5 caatagcaaaCTTGTACGAAAtatgtgtaca nd [GEA], [APIORCISFBSCS], [BPP] [2]


Evidence    

 [IHBCE] Inferred by a human based on computational evidence

 [TIM] Transcription initiation mapping

 [GEA] Gene expression analysis

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BPP] Binding of purified proteins


Reference(s)    

 [1] Tschowri N., Lindenberg S., Hengge R., 2012, Molecular function and potential evolution of the biofilm-modulating blue light-signalling pathway of Escherichia coli., Mol Microbiol 85(5):893-906

 [2] Shimada T., Katayama Y., Kawakita S., Ogasawara H., Nakano M., Yamamoto K., Ishihama A., 2012, A novel regulator RcdA of the csgD gene encoding the master regulator of biofilm formation in Escherichia coli., Microbiologyopen 1(4):381-94

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