RegulonDB RegulonDB 11.2: Operon Form
   

dgcZ operon and associated TUs in Escherichia coli K-12 genome


Transcription unit          
Name: dgcZ
Gene(s): dgcZ   Genome Browser M3D Gene expression COLOMBOS
Note(s): Base on a σS-deficient rpoS mutant background, ydeH is under the significant negative control of rpoS Sommerfeldt N,2009 This negative control is most likely due to increased formation of a vegetative or other alternative RNAP holoenzyme in the absence of competition of σS with the other sigma factors Sommerfeldt N,2009 This suggests that σS might control ydeH expression indirectly.
The expression of the gene ydeH is increased when colicin (at 30 ng/ml) is added to the medium Kamen?ek S, ?gur-Bertok D,2013.
Under NlpE outer membrane lipoprotein overexpression, the transcription of the ydeH gene is increased by CpxR Raivio TL,2013
Evidence: [COMP-AINF] Inferred computationally without human oversight
[EXP-IDA-BOUNDARIES-DEFINED] Boundaries of transcription experimentally identified
Reference(s): [1] Jonas K., et al., 2008
Promoter
Name: dgcZp
+1: 1623879
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 29
Sequence: aacttcggatttctatgaaaatattgacgtaattttgcttttctctcgttagaatagcgcGcacaaggaactgtgaaaaag
                             -35                       -10  +1                   
Evidence: [COMP-AINF]
[COMP-HINF]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
[EXP-IDA-HPT-TRANSCR-INIT-M-RACE-MAP]
[EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
[RS-EPT-CBR]
Reference(s): [2] Huerta AM., et al., 2003
[1] Jonas K., et al., 2008
[3] Mendoza-Vargas A., et al., 2009
[4] Salgado H, et al., 2012
[5] Yamamoto K., et al., 2006
TF binding sites (TFBSs)
[CpxR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CpxR-phosphorylated activator dgcZp 1623912 1623929 -41.0 aacttcggatTTCTATGAAAATATTGACgtaattttgc nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] W [5], [7]
[CysB,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CysB-O-acetyl-L-serine activator dgcZp 1624023 1624065 -164.5 tgggccattaGGGTATTGGGTTATGCTATTTGTCATCCTTTTATTTACGATAAtgcagaggta nd [COMP-AINF-SIMILAR-TO-CONSENSUS] nd [6]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [COMP-HINF] Inferred by a human based on computational evidence

 [COMP-HINF-POSITIONAL-IDENTIFICATION] Human inference of promoter position

 [EXP-IDA-HPT-TRANSCR-INIT-M-RACE-MAP] High-throughput transcription initiation mapping

 [EXP-IDA-TRANSCRIPTION-INIT-MAPPING] Transcription initiation mapping

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis

 [COMP-HINF-SIMILAR-TO-CONSENSUS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins

 [COMP-AINF-SIMILAR-TO-CONSENSUS] Automated inference based on similarity to consensus sequences


Reference(s)    

 [1] Jonas K., Edwards AN., Simm R., Romeo T., Romling U., Melefors O., 2008, The RNA binding protein CsrA controls cyclic di-GMP metabolism by directly regulating the expression of GGDEF proteins., Mol Microbiol 70(1):236-57

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Mendoza-Vargas A., Olvera L., Olvera M., Grande R., Vega-Alvarado L., Taboada B., Jimenez-Jacinto V., Salgado H., Juarez K., Contreras-Moreira B., Huerta AM., Collado-Vides J., Morett E., 2009, Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli., PLoS One 4(10):e7526

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [5] Yamamoto K., Ishihama A., 2006, Characterization of copper-inducible promoters regulated by CpxA/CpxR in Escherichia coli., Biosci Biotechnol Biochem 70(7):1688-95

 [6] Kawano Y., Ohtsu I., Takumi K., Tamakoshi A., Nonaka G., Funahashi E., Ihara M., Takagi H., 2015, Enhancement of L-cysteine production by disruption of yciW in Escherichia coli., J Biosci Bioeng 119(2):176-9

 [7] Raivio TL., Leblanc SK., Price NL., 2013, The Escherichia coli Cpx envelope stress response regulates genes of diverse function that impact antibiotic resistance and membrane integrity., J Bacteriol 195(12):2755-67

RegulonDB