RegulonDB RegulonDB 10.8: Operon Form
   

pdeL operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: pdeL
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: yahA
Gene(s): pdeL   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Shimada T., et al., 2005
Promoter
Name: pdeLp2
+1: 331873
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 498
Sequence: tttattgaatgttttaaatattgtttttattggcattgctataatattggttatcatttgCtgaatggattcagtcttaat
                              -35                   -10     +1                   
Evidence: [IDA]
Reference(s): [1] Shimada T., et al., 2005
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote PdeL activator pdeLp2 331672 331696 -189.0 gttgcgaatgTTCAATAAGTTTAGTCTTATTTAATgtaaatattg nd [BPP], [CV(GEA)], [CV(GEA)], [GEA], [IHBCE] [2]


Transcription unit          
Name: yahA
Gene(s): pdeL   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
[ICWHO] Inferred computationally without human oversight
Reference(s): [1] Shimada T., et al., 2005
Promoter
Name: pdeLp1
+1: 332039
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 332
Sequence: ctatgtgtttatgagggagaggtattttcagttgatctggattgttaaattcatataatgCgcctttgctcatgaatggat
                      -35                          -10      +1                   
Evidence: [HTIM]
[IDA]
Reference(s): [3] Maciag A., et al., 2011
[1] Shimada T., et al., 2005
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote Cra repressor pdeLp1 331871 331888 -159.5 ggttatcattTGCTGAATGGATTCAGTCttaatgagtg nd [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [1]
proximal Cra repressor pdeLp1 332017 332034 -13.5 cagttgatctGGATTGTTAAATTCATATaatgcgcctt nd [AIBSCS], [CV(GEA)], [GEA] [1]


Transcription unit       
Name: pdeL
Gene(s): pdeL   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Shimada T., et al., 2005
Promoter
Name: pdeLp3
+1: 332043
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 328
Sequence: gtgtttatgagggagaggtattttcagttgatctggattgttaaattcatataatgcgccTttgctcatgaatggatgcca
                                                  -10       +1                   
Note(s): Based on ChIp-chip analysis, it was shown that H-NS plays a role in pdeL expression 19151137
Transcription directed across H-NS binding regions relieves H-NS-mediated (and H-NS/StpA-mediated) repression of promoters such as proVp3, pdeLp3, and appYp2, and there is a correlation between transcription across the H-NS-bound region and derepression where RNA polymerase is able to remodel the H-NS complex and/or dislodge H-NS from the DNA and thus relieve repression Rangarajan AA,2018
Evidence: [TIM]
Reference(s): [4] Rangarajan AA., et al., 2018





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