RegulonDB RegulonDB 10.8: Operon Form
   

dgcC operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: dgcC
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: adrA
Synonym(s): yaiC
Gene(s): dgcC   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Brombacher E., et al., 2003
Promoter
Name: adrAp
+1: 403687
Sigma Factor: Sigma38 Sigmulon
Distance from start of the gene: 16
Sequence: tacgaaaatggctcacccgaaatatcatacttctgcctttagctccgtctctataatttgGgaaaattgtttctgaatgtt
                               -35             -10          +1                   
Note(s): The adrAp promoter has a -10 box perfectly matching the boxes for σ70 promoters, but it does not have a -35 box, which is consistent with the fact that it is a promoter that is activated Brombacher E,2003 .
Evidence: [HIPP]
[TIM]
Reference(s): [1] Brombacher E., et al., 2003
[2] Weber H., et al., 2006
TF binding sites (TFBSs)
[CsgD,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CsgD activator adrAp 403615 403629 -65.5 ctgatagtgcTAACGGGTGAGCTACgaaaatggct nd [AIBSCS], [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] [1], [3]
proximal CsgD2 activator adrAp 403635 403645 -47.0 gctacgaaaaTGGCTCACCCGaaatatcata nd [AIBSCS], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] [1]
Note(s): 2The CsgD protein is bound to two sites (-47 and -64) upstream of the adrAp promoter. Together, these sites form an inverted repeat, suggesting that CsgD binds to this region as a dimer. In other regulatory regions, the CsgD protein binds to just one site Brombacher E,2003.2The CsgD protein is bound to two sites (-47 and -64) upstream of the adrAp promoter. Together, these sites form an inverted repeat, suggesting that CsgD binds to this region as a dimer. In other regulatory regions, the CsgD protein binds to just one site Brombacher E,2003.


Evidence    

 [HIPP] Human inference of promoter position

 [TIM] Transcription initiation mapping

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BPP] Binding of purified proteins

 [CV(GEA)] cross validation(GEA)

 [CV(GEA/SM)] cross validation(GEA/SM)

 [CV(SM)] cross validation(SM)

 [GEA] Gene expression analysis

 [SM] Site mutation


Reference(s)    

 [1] Brombacher E., Dorel C., Zehnder AJ., Landini P., 2003, The curli biosynthesis regulator CsgD co-ordinates the expression of both positive and negative determinants for biofilm formation in Escherichia coli., Microbiology 149(Pt 10):2847-57

 [2] Weber H., Pesavento C., Possling A., Tischendorf G., Hengge R., 2006, Cyclic-di-GMP-mediated signalling within the sigma network of Escherichia coli., Mol Microbiol 62(4):1014-34

 [3] Ogasawara H., Yamamoto K., Ishihama A., 2011, Role of the biofilm master regulator CsgD in cross-regulation between biofilm formation and flagellar synthesis., J Bacteriol 193(10):2587-97

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