RegulonDB RegulonDB 10.7: Operon Form
   

sdsN operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: sdsN
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: sdsN
Gene(s): sdsN   Genome Browser M3D Gene expression COLOMBOS
Note(s): sdsN is induced during stationary phase at low temperature or with preferred nitrogen sources through the Crl and NarP transcription factors, respectively |CITS:[27166377]|. SdsN represses genes involved in the metabolism of oxidized nitrogen compounds. In addition, sdsN confers resistance to nitrofurans through repression of nfsA |CITS:[27166377]|.
Several transcripts were detected in the tcyN-sdiA intergenic region during stationary phase. The two most prominent bands were approximately 180 nt (SdsN178) and 140 nt long (SdsN137) and shared the same Rho-independent terminator. Another band was identified and shown to be a processed transcript of approximately 120 nt (SdsN124) |CITS:[27166377]|.
The Hfq chaperone binds to SdsN178, SdsN137, and SdsN124 with different affinities |CITS:[27166377]|.
SdsN178
SdsN178 overproduction affected one mRNA, casA, which showed a 2-fold increase and only partial of nfsA,hmpA, and narP. The 5??-end sequence of SdsN178 is extremely AU rich |CITS:[27166377]|.
SdsN137
SdsN137 is transcribed from a sigma&;S-dependent promoter. Overexpression regulates negatively four mRNAs related to the metabolism of and response to nitrogen compounds: nfsA, hmpA, ybjC, and the response regulator narP. Other transcripts are regulated by SdsN137 and do not fall into a single category: cya, uraA, inaA, and rluA are downregulated and fliC and ymfT are upregulated. nfsA, hmpA, and narP were identified as base pairs directly regulated by SdsN137 |CITS:[27166377]|.
The SdsN137 transcript could be also a processed transcript, since it is within the sdsN gene. There, a Rho-independent terminator with the absolute cordinates 1997022 to 1997057 falls within the sdsN gene.
SdsN124
SdsN124 is a processed form from SdsN178 and SdsN137 transcripts and accumulates maximally after 20 h of growth in M63 glucose medium. SdsN124 represses nfsA and hmpA |CITS:[27166377]|.
Evidence: [BTEI] Boundaries of transcription experimentally identified
[LTED] Length of transcript experimentally determined
Reference(s): [1] Hao Y., et al., 2016
[2] Raghavan R., et al., 2011
Promoter
Name: sdsNp2
+1: 1996880
Sigma Factor: Sigma38 Sigmulon
Distance from start of the gene: 41
Sequence: ccttatcctgcatagtaaaccgcaacgcccctgagagtgagtatcatttataatgataatTcaaactatcacataaataag
                          -35                    -10        +1                   
Evidence: [TIM]
Reference(s): [1] Hao Y., et al., 2016


Transcription unit       
Name: sdsN
Gene(s): sdsN   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
[LTED] Length of transcript experimentally determined
Reference(s): [1] Hao Y., et al., 2016
[2] Raghavan R., et al., 2011
Promoter
Name: sdsNp
+1: 1996906
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 15
Sequence: gcccctgagagtgagtatcatttataatgataattcaaactatcacataaataagatttaTatataatttatattattcag
Evidence: [TIM]
Reference(s): [2] Raghavan R., et al., 2011





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