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| Operon |  |
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| Name: | sdsN |
| This page displays every known transcription unit of this operon and their known regulation. | |
| Name: | sdsN |
| Gene(s): | sdsN Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | sdsN is induced during stationary phase at low temperature or with preferred nitrogen sources through the Crl and NarP transcription factors, respectively Hao Y,2016 SdsN represses genes involved in the metabolism of oxidized nitrogen compounds. In addition, sdsN confers resistance to nitrofurans through repression of nfsA Hao Y,2016 Several transcripts were detected in the tcyN-sdiA intergenic region during stationary phase. The two most prominent bands were approximately 180 nt (SdsN178) and 140 nt long (SdsN137) and shared the same Rho-independent terminator. Another band was identified and shown to be a processed transcript of approximately 120 nt (SdsN124) Hao Y,2016 The Hfq chaperone binds to SdsN178, SdsN137, and SdsN124 with different affinities Hao Y,2016 SdsN178 SdsN178 overproduction affected one mRNA, casA, which showed a 2-fold increase and only partial of nfsA,hmpA, and narP. The 5´-end sequence of SdsN178 is extremely AU rich Hao Y,2016 SdsN137 SdsN137 is transcribed from a sigma&;S-dependent promoter. Overexpression regulates negatively four mRNAs related to the metabolism of and response to nitrogen compounds: nfsA, hmpA, ybjC, and the response regulator narP. Other transcripts are regulated by SdsN137 and do not fall into a single category: cya, uraA, inaA, and rluA are downregulated and fliC and ymfT are upregulated. nfsA, hmpA, and narP were identified as base pairs directly regulated by SdsN137 Hao Y,2016 The SdsN137 transcript could be also a processed transcript, since it is within the sdsN gene. There, a Rho-independent terminator with the absolute cordinates 1997022 to 1997057 falls within the sdsN gene. SdsN124 SdsN124 is a processed form from SdsN178 and SdsN137 transcripts and accumulates maximally after 20 h of growth in M63 glucose medium. SdsN124 represses nfsA and hmpA Hao Y,2016 |
| Evidence: | [BTEI] Boundaries of transcription experimentally identified [LTED] Length of transcript experimentally determined |
| Reference(s): |
[1] Hao Y., et al., 2016 [2] Raghavan R., et al., 2011 |
| Promoter | |
| Name: | sdsNp2 |
| +1: | 1996880 |
| Sigma Factor: | Sigma38 Sigmulon |
| Distance from start of the gene: | 41 |
| Sequence: |
ccttatcctgcatagtaaaccgcaacgcccctgagagtgagtatcatttataatgataatTcaaactatcacataaataag -35 -10 +1 |
| Evidence: | [TIM] |
| Reference(s): | [1] Hao Y., et al., 2016 |
| Name: | sdsN |
| Gene(s): | sdsN Genome Browser M3D Gene expression COLOMBOS |
| Evidence: | [BTEI] Boundaries of transcription experimentally identified [LTED] Length of transcript experimentally determined |
| Reference(s): |
[1] Hao Y., et al., 2016 [2] Raghavan R., et al., 2011 |
| Promoter | |
| Name: | sdsNp |
| +1: | 1996906 |
| Sigma Factor: | Sigma70 Sigmulon |
| Distance from start of the gene: | 15 |
| Sequence: |
gcccctgagagtgagtatcatttataatgataattcaaactatcacataaataagatttaTatataatttatattattcag |
| Evidence: | [TIM] |
| Reference(s): | [2] Raghavan R., et al., 2011 |
| Reference(s) |
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|---|---|
[TIM] Transcription initiation mapping