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| Operon |  |
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| Name: | sdsN |
| This page displays every known transcription unit of this operon and their known regulation. | |
| Name: | sdsN |
| Gene(s): | sdsN Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | sdsN is induced during stationary phase at low temperature or with preferred nitrogen sources through the Crl and NarP transcription factors, respectively |CITS:[27166377]|. SdsN represses genes involved in the metabolism of oxidized nitrogen compounds. In addition, sdsN confers resistance to nitrofurans through repression of nfsA |CITS:[27166377]|. Several transcripts were detected in the tcyN-sdiA intergenic region during stationary phase. The two most prominent bands were approximately 180 nt (SdsN178) and 140 nt long (SdsN137) and shared the same Rho-independent terminator. Another band was identified and shown to be a processed transcript of approximately 120 nt (SdsN124) |CITS:[27166377]|. The Hfq chaperone binds to SdsN178, SdsN137, and SdsN124 with different affinities |CITS:[27166377]|. SdsN178 SdsN178 overproduction affected one mRNA, casA, which showed a 2-fold increase and only partial of nfsA,hmpA, and narP. The 5??-end sequence of SdsN178 is extremely AU rich |CITS:[27166377]|. SdsN137 SdsN137 is transcribed from a sigma&;S-dependent promoter. Overexpression regulates negatively four mRNAs related to the metabolism of and response to nitrogen compounds: nfsA, hmpA, ybjC, and the response regulator narP. Other transcripts are regulated by SdsN137 and do not fall into a single category: cya, uraA, inaA, and rluA are downregulated and fliC and ymfT are upregulated. nfsA, hmpA, and narP were identified as base pairs directly regulated by SdsN137 |CITS:[27166377]|. The SdsN137 transcript could be also a processed transcript, since it is within the sdsN gene. There, a Rho-independent terminator with the absolute cordinates 1997022 to 1997057 falls within the sdsN gene. SdsN124 SdsN124 is a processed form from SdsN178 and SdsN137 transcripts and accumulates maximally after 20 h of growth in M63 glucose medium. SdsN124 represses nfsA and hmpA |CITS:[27166377]|. |
| Evidence: | [BTEI] Boundaries of transcription experimentally identified [LTED] Length of transcript experimentally determined |
| Reference(s): |
[1] Hao Y., et al., 2016 [2] Raghavan R., et al., 2011 |
| Promoter | |
| Name: | sdsNp2 |
| +1: | 1996880 |
| Sigma Factor: | Sigma38 Sigmulon |
| Distance from start of the gene: | 41 |
| Sequence: |
ccttatcctgcatagtaaaccgcaacgcccctgagagtgagtatcatttataatgataatTcaaactatcacataaataag -35 -10 +1 |
| Evidence: | [TIM] |
| Reference(s): | [1] Hao Y., et al., 2016 |
| Name: | sdsN |
| Gene(s): | sdsN Genome Browser M3D Gene expression COLOMBOS |
| Evidence: | [BTEI] Boundaries of transcription experimentally identified [LTED] Length of transcript experimentally determined |
| Reference(s): |
[1] Hao Y., et al., 2016 [2] Raghavan R., et al., 2011 |
| Promoter | |
| Name: | sdsNp |
| +1: | 1996906 |
| Sigma Factor: | Sigma70 Sigmulon |
| Distance from start of the gene: | 15 |
| Sequence: |
gcccctgagagtgagtatcatttataatgataattcaaactatcacataaataagatttaTatataatttatattattcag |
| Evidence: | [TIM] |
| Reference(s): | [2] Raghavan R., et al., 2011 |
| Reference(s) |
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|---|---|
[TIM] Transcription initiation mapping