|Gene(s):||dinG, ybiB Genome Browser M3D Gene expression COLOMBOS|
|Evidence:||[IEP] Inferred from expression pattern
[LTED] Length of transcript experimentally determined
 Kaleta C., et al., 2010
 Schneider D., et al., 2015
|Sigma Factor:||Sigma70 Sigmulon|
|Distance from start of the gene:||-3|
-35 -10 +1
|Note(s):||We assigned a putative transcription start site to this promoter based on the observation that the majority of the promoters, whose transcription start sites were determined experimentally, present a distance of 6 nucleotides between the transcription start site and the -10 box.
Lewis LK,1992predicted a potential dinG promoter site (-35 and -10 regions) by using three different computational search programs. The putative transcription start site of this promoter overlaps the dinG gene, but the start of the translation of this gene has not been determined.
|Reference(s):|| Lewis LK., et al., 1992|
|Type||Transcription factor||Function||Promoter||Binding Sites||Growth Conditions||Evidence (Confirmed, Strong, Weak)||Reference(s)|
|proximal||LexA1||repressor||dinGp||833036||833055||-27.5||aatgccacaaTATTGGCTGTTTATACAGTAtttcaggttt||nd||[AIBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA]||, |
|Note(s):||1The dinG sequence breaks the pattern of the binding site for the LexA repressor, since it takes the form TTG(N10)CAG instead of CTG(N10)CAG.1The dinG sequence breaks the pattern of the binding site for the LexA repressor, since it takes the form TTG(N10)CAG instead of CTG(N10)CAG.|
|Gene(s):||ybiB Genome Browser M3D Gene expression COLOMBOS|
|Note(s):||In conjunction with gene expression analysis, it can be argued that the ybiB and dinG genes form an operon regulated by LexA Kaleta C,2010
Based on crystallization analysis and EMSA, it was determined that YbiB binds to DNA with high affinity. The ybiB gene is under LexA control and is induced by DNA-damaging agents Schneider D,2015.
|Evidence:||[IHBCE] Inferred by a human based on computational evidence|
 Kaleta C., Gohler A., Schuster S., Jahreis K., Guthke R., Nikolajewa S., 2010, Integrative inference of gene-regulatory networks in Escherichia coli using information theoretic concepts and sequence analysis., BMC Syst Biol 4:116
 Schneider D., Kaiser W., Stutz C., Holinski A., Mayans O., Babinger P., 2015, YbiB from Escherichia coli, the Defining Member of the Novel TrpD2 Family of Prokaryotic DNA-binding Proteins., J Biol Chem 290(32):19527-39