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CpxR DNA-binding transcriptional dual regulator

Synonyms: CpxR-Phosphorylated, CpxR
Summary:
The regulatory protein CpxR appears to control at least 100 operons [5] Some of these operons are involved in cellular functions, such as
"conjugative plasmid gene expression" (cpx) [], the envelope stress response system, pilus assembly, secretion, motility and chemotaxis, adherence, biofilm development, multidrug resistance and efflux [24], and the copper-responsive regulatory system [1], among others. In pathogenic bacteria it controls genes involved in invasion of host cells []. Sometimes, CpxR acts to modulate the action of the main activators or repressors of some promoters [24]. CpxR is the response regulator in the two-component regulatory system comprised of CpxR and CpxA [25].
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Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
CpxR Non-Functional   Apo [BPP] [1]
CpxR-Phosphorylated Functional Covalent Holo [BPP], [IPI] [1], [2], [3]
Evolutionary Family: OmpR
Sensing class: External-Two-component systems
Connectivity class: Local Regulator
Gene name: cpxR
  Genome position: 4104972-4105670
  Length: 699 bp / 232 aa
Operon name: cpxRA
TU(s) encoding the TF:
Transcription unit        Promoter
cpxRA
cpxRp


Regulon       
Regulated gene(s) acrD, aroG, bacA, baeR, baeS, bamE, cheA, cheW, cpxA, cpxP, cpxQ, cpxR, csgA, csgB, csgC, csgD, csgE, csgF, csgG, cyaR, degP, dgcZ, dsbA, dsbC, efeU_1, efeU_2, fabZ, ftnB, hha, kbp, ldtC, ldtD, lpxA, lpxD, marA, marB, marR, mdtA, mdtB, mdtC, mdtD, motA, motB, mscM, mzrA, ompC, ompF, ppiA, ppiD, psd, rpoE, rpoH, rprA, rseA, rseB, rseC, rseD, sbmA, skp, slt, spy, srkA, tomB, tsr, ung,
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Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
membrane (21)
Transcription related (13)
other (mechanical, nutritional, oxidative stress) (8)
chaperoning, repair (refolding) (7)
drug resistance/sensitivity (6)
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Regulated operon(s) acrD, aroG, bacA, bamA-skp-lpxD-fabZ-lpxAB-rnhB-dnaE, bamE, cpxPQ, cpxRA, csgBAC, csgDEFG, cyaR, degP, dgcZ, efeU_1U_2, ftnB, ldtC, ldtD, marRAB, mdtABCD-baeSR, motAB-cheAW, ompC, ompF, ppiA, ppiD, psd-mscM, rpoH, rprA, rseD-rpoE-rseABC, sbmA-yaiW, slt, spy, srkA-dsbA, tomB-hha, tsr, ung, xerD-dsbC-recJ-prfB-lysS, yccA, yebE, yidQ, yqaE-kbp, yqjA-mzrA
First gene in the operon(s) acrD, aroG, bacA, bamE, cpxP, cpxR, csgB, csgD, cyaR, degP, dgcZ, dsbC, efeU_1, ftnB, ldtC, ldtD, marR, mdtA, motA, ompC, ompF, ppiA, ppiD, psd, rpoH, rprA, rseD, sbmA, skp, slt, spy, srkA, tomB, tsr, ung, yccA, yebE, yidQ, yqaE, yqaE, yqjA
Simple and complex regulons AcrR,CRP,CpxR,Cra,Fis,MarA,MarR,Rob,SoxS
BaeR,CpxR
BasR,CRP,CpxR,Cra,CsgD,FliZ,H-NS,IHF,MlrA,MqsA,OmpR,RcdA,RcsAB,RstA
BasR,CpxR,PdhR
CRP,CpxR
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Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[CpxR,+](26)
[CpxR,-](10)


Transcription factor regulation    


Transcription factor binding sites (TFBSs) arrangements
      

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence
 LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  CpxR-Phosphorylated activator acrDp2 Sigma54 -127.5 -172.0 acrD
ggagggtgagGAAAGTCAGTTAATGTAAtgcctcctac
 2587414 2587432 [BPP], [GEA], [IC] [4]
  CpxR-Phosphorylated activator acrDp2 Sigma54 -98.0 -142.5 acrD
tcctactgacCAAAGAATACTTGcacttaaggt
 2587446 2587459 [BPP], [IC] [4]
  CpxR-Phosphorylated activator acrDp2 Sigma54 -66.0 -110.5 acrD
ggttcagtatAAAAGGGCATGATAATTtacattaact
 2587476 2587493 [BPP], [GEA], [IC] [4]
  CpxR-Phosphorylated activator aroGp Sigma70 -38.0 -80.0 aroG
cgttcatagtGTAAAACCCCGTTTAcacattctga
 785546 785560 [BPP], [HIBSCS] [1]
  CpxR-Phosphorylated activator bacAp Sigma24 -292.0 -336.0 bacA
acatcatcacTTTTCGCCGCTTTACggttatccca
 3204460 3204474 [ICA] [5]
  CpxR-Phosphorylated repressor bamEp Sigma24 11.0 -109.0 bamE
agagtaaacaGCAAAACGCCGTAAGaccggaaagc
 2753489 2753503 [ICA] [5]
  CpxR-Phosphorylated activator cpxPp Sigma70 -52.0 -87.0 cpxP, cpxQ
gtcagggggtGTAAAACAACGTAAAgtcatggatt
 4105726 4105740 [BPP], [GEA], [HIBSCS] [1], [2], [6], [7]
  CpxR-Phosphorylated activator cpxPp Sigma70 -32.0 -67.0 cpxP, cpxQ
tttacaccccCTGACGCATGTTTGCagcctgaatc
 4105746 4105760 [GEA], [HIBSCS] [6], [7]
  CpxR-Phosphorylated activator cpxPp Sigma70 -5.0 -39.5 cpxP, cpxQ
agcctgaatcGTAAACTCTCTATCGTTGAatcgcgacag
 4105771 4105790 [BPP], [GEA], [HIBSCS] [1], [6], [7]
  CpxR-Phosphorylated activator cpxRp Sigma38, Sigma70 -67.0 -83.0 cpxR, cpxA
gattcaggctGCAAACATGCGTCAGggggtgtaaa
 4105746 4105760 [GEA], [HIBSCS] [6], [7]
  CpxR-Phosphorylated activator cpxRp Sigma38, Sigma70 -47.0 -63.0 cpxR, cpxA
aatccatgacTTTACGTTGTTTTACaccccctgac
 4105726 4105740 [BPP], [GEA], [HIBSCS] [1], [2], [6], [7]
  CpxR-Phosphorylated repressor csgBp Sigma38, Sigma70, Sigma38 -464.0 -556.0 csgB, csgA, csgC
gtgttaaacaTGTAACTAAATGTAActcgttatat
 1103388 1103402 [BPP], [GEA], [HIBSCS] [8]
  CpxR-Phosphorylated repressor csgBp Sigma38, Sigma70, Sigma38 -255.0 -347.0 csgB, csgA, csgC
cacacctccgTGGACAATTTTTTACtgcaaaaaga
 1103597 1103611 [HIBSCS] [5]
  CpxR-Phosphorylated repressor csgBp Sigma38, Sigma70, Sigma38 8.0 -85.0 csgB, csgA, csgC
actttccatcGTAACGCAGCGTTAAcaaaatacag
 1103859 1103873 [AIBSCS], [BPP], [GEA], [HIBSCS] [5], [8]
  CpxR-Phosphorylated repressor csgDp1 Sigma38, Sigma70 -186.0 -334.0 csgD, csgE, csgF, csgG
aacttactaaTAATGCATATAAAAAatatttcggt
 1103523 1103537 [BPP], [GEA], [HIBSCS] [9]
  CpxR-Phosphorylated repressor csgDp1 Sigma38, Sigma70 -178.0 -326.5 csgD, csgE, csgF, csgG
tggtgataacTTACTAATAATGCatataaaaaa
 1103516 1103529 [BPP], [GEA], [HIBSCS] [9]
  CpxR-Phosphorylated repressor csgDp1 Sigma38, Sigma70 -166.0 -314.0 csgD, csgE, csgF, csgG
aaaatcatacAAATGGTGATAACTTactaataatg
 1103503 1103517 [BPP], [GEA], [HIBSCS] [9]
  CpxR-Phosphorylated repressor csgDp1 Sigma38, Sigma70 -141.0 -289.0 csgD, csgE, csgF, csgG
tttggtttttATTGCACAATTTTAAaaaatcatac
 1103478 1103492 [BPP], [GEA], [HIBSCS] [9]
  CpxR-Phosphorylated repressor csgDp1 Sigma38, Sigma70 -129.5 -278.0 csgD, csgE, csgF, csgG
aaaagttgtaCATTTGGTTTTTATTGcacaatttta
 1103466 1103482 [BPP], [GEA], [HIBSCS] [9]
  CpxR-Phosphorylated repressor csgDp1 Sigma38, Sigma70 -108.5 -257.0 csgD, csgE, csgF, csgG
attaagtttaGAAATGATAGAAAAgttgtacatt
 1103446 1103460 [BPP], [GEA], [HIBSCS] [9]
  CpxR-Phosphorylated repressor csgDp1 Sigma38, Sigma70 -100.0 -248.0 csgD, csgE, csgF, csgG
taaggttttaTTAAGTTTAGAAATGatagaaaagt
 1103437 1103451 [BPP], [GEA], [HIBSCS] [9], [10]
  CpxR-Phosphorylated repressor csgDp1 Sigma38, Sigma70 -95.5 -244.0 csgD, csgE, csgF, csgG
accttaaggtTTTATTAAGTTTAGaaatgataga
 1103433 1103447 [BPP], [GEA], [HIBSCS] [9]
  CpxR-Phosphorylated repressor csgDp1 Sigma38, Sigma70 -73.5 -222.0 csgD, csgE, csgF, csgG
aactcgttatATTAAAATGTTAACcttaaggttt
 1103411 1103425 [BPP], [GEA], [HIBSCS] [9]
  CpxR-Phosphorylated repressor csgDp1 Sigma38, Sigma70 -52.0 -200.0 csgD, csgE, csgF, csgG
tgttaaacatGTAACTAAATGTAACtcgttatatt
 1103389 1103403 [BPP], [GEA], [HIBSCS] [8], [10]
  CpxR-Phosphorylated repressor csgDp1 Sigma38, Sigma70 -48.0 -196.0 csgD, csgE, csgF, csgG
caagtgttaaACATGTAACTAAATGtaactcgtta
 1103385 1103399 [BPP], [GEA], [HIBSCS] [10]
  CpxR-Phosphorylated repressor csgDp1 Sigma38, Sigma70 -43.0 -191.0 csgD, csgE, csgF, csgG
taaatcaagtGTTAAACATGTAACTaaatgtaact
 1103380 1103394 [BPP], [GEA], [HIBSCS] [9]
  CpxR-Phosphorylated repressor csgDp1 Sigma38, Sigma70 -21.5 -170.0 csgD, csgE, csgF, csgG
caatctagccATTACAAATCTTAAatcaagtgtt
 1103359 1103373 [BPP], [GEA], [HIBSCS] [9], [10]
  CpxR-Phosphorylated repressor csgDp1 Sigma38, Sigma70 3.0 -146.0 csgD, csgE, csgF, csgG
aaactaatggATTACATCTGATTTCaatctagcca
 1103335 1103349 [BPP], [GEA], [HIBSCS] [10]
  CpxR-Phosphorylated repressor cyaRp Sigma24, Sigma70, Sigma38 nd -2167114.0 cyaR
nd
 nd nd [BPP], [GEA] [11]
  CpxR-Phosphorylated activator degPp Sigma24 -223.0 -262.0 degP
gaggctgtcaGTAAATTACCGTCAGattctcctga
 180615 180629 [BPP], [GEA] [2], [12]
  CpxR-Phosphorylated activator degPp Sigma24 -71.5 -110.0 degP
ccaggcttttGTAAAGACGAACAATAAAtttttacctt
 180765 180783 [AIBSCS], [BPP], [GEA], [HIBSCS] [1], [2], [12]
  CpxR-Phosphorylated activator degPp Sigma24 -63.0 -102.0 degP
gtaaagacgaACAATAAATTTTTACcttttgcaga
 180775 180789 [BPP], [GEA], [HIBSCS] [2], [12]
  CpxR-Phosphorylated activator dgcZp Sigma70 -41.0 -70.5 dgcZ
gcaaaattacGTCAATATTTTCATAGAaatccgaagt
 1623912 1623929 [BPP], [GEA], [HIBSCS] [1], [7]
  CpxR-Phosphorylated activator dsbCp Sigma24 -391.5 -404.0 dsbC
ccagacgctcTTTGTTGCCTTTACcaatgacccg
 3039219 3039233 [GEA], [HIBSCS] [13]
  CpxR-Phosphorylated activator dsbCp Sigma24 -357.5 -369.0 dsbC
ttccagccagTAAACCGCCTCTTCAcctaacggca
 3039184 3039198 [GEA], [HIBSCS] [13]
  CpxR-Phosphorylated repressor efeU_1p Sigma70 -54.5 -103.0 efeU_1, efeU_2
ccaacatcatTTTATAAACATTCCgcttgtgttt
 1081246 1081260 [BPP], [GEA], [HIBSCS] [1], [7]
  CpxR-Phosphorylated activator ftnBp2 Sigma70 -61.0 -187.0 ftnB
tcaatgaaatGTAAAAATATATAAActtgatgatt
 1986731 1986745 [BPP], [HIBSCS] [1]
  CpxR-Phosphorylated activator hlpAp Sigma24 -101.0 -128.0 skp, lpxD, fabZ, lpxA
tttaacatcgGTAAAACCTGGTAAGtgttctccac
 200347 200361 [GEA], [HIBSCS] [13]
  CpxR-Phosphorylated activator ldtDp nd -192.0 -307.5 ldtD
gatttgcgccGCAACTCCCTGAAACgcttccagga
 980732 980747 [BPP], [GEA], [HIBSCS] [7], [14]
  CpxR-Phosphorylated activator marRp Sigma70 -29.0 -55.5 marR, marA, marB
attcattcatTTGACTTATACTTGCctgggcaata
 1619057 1619072 [BPP], [GEA], [HIBSCS] [15]
  CpxR-Phosphorylated activator mdtAp Sigma38 -128.5 -165.0 mdtA, mdtB, mdtC, mdtD, baeS, baeR
cagtctatcgCTAACGCGTAGATAAAatagtttcct
 2153843 2153859 [BPP], [GEA], [IC] [4]
  CpxR-Phosphorylated activator mdtAp Sigma38 -66.5 -103.0 mdtA, mdtB, mdtC, mdtD, baeS, baeR
atgtgcccgtCATTCAGACGATTCcagacagtgt
 2153906 2153920 [BPP], [GEA], [IC] [4]
  CpxR-Phosphorylated repressor motAp Sigma28 -37.0 -200.0 motA, motB, cheA, cheW
tctgggaaagTTTACGTCTTTTTACtgcccgggat
 1977332 1977346 [BPP], [GEA] [5], [6]
  CpxR-Phosphorylated activator ompCp1 Sigma70 -351.0 -432.0 ompC
ttcggtttttTTTACCCTTCTTTACacacttttca
 2313174 2313188 [AIBSCS], [BPP], [GEA] [16]
  CpxR-Phosphorylated activator ompCp1 Sigma70 -134.0 -215.0 ompC
gattattgcgGAATGGCGAAATAAGcacctaacat
 2312957 2312971 [AIBSCS], [BPP], [GEA] [16]
  CpxR-Phosphorylated activator ompCp1 Sigma70 -95.0 -176.5 ompC
agatgtttcaAAATGTAAATGcaagggaact
 2312920 2312931 [AIBSCS], [BPP], [GEA] [16]
  CpxR-Phosphorylated repressor ompFp Sigma38, Sigma70 -429.5 -540.0 ompF
attgcttgacTTAACAAACAGATAACttgacagaaa
 987514 987530 [AIBSCS], [BPP], [GEA] [7], [16]
  CpxR-Phosphorylated repressor ompFp Sigma38, Sigma70 -373.0 -483.0 ompF
atgttgcaatGTAATATTCCGTAACaggatgatcg
 987458 987472 [AIBSCS], [BPP], [GEA] [7], [16]
  CpxR-Phosphorylated repressor ompFp Sigma38, Sigma70 -93.0 -203.0 ompF
gaaaaaatatGTAACCAAAAGTAAAatttaaggaa
 987178 987192 [AIBSCS], [BPP], [GEA] [7], [16]
  CpxR-Phosphorylated repressor ompFp Sigma38, Sigma70 -68.5 -179.0 ompF
aaagataaagATTTGGTTTCAAAAAGaaaaaatatg
 987153 987169 [AIBSCS], [BPP], [GEA] [7], [16]
  CpxR-Phosphorylated activator ppiAp1 Sigma70 -47.0 -85.0 ppiA
aggccatcccTTTACCTAATTTTACgtatctgaaa
 3492375 3492389 [BPP], [GEA], [HIBSCS] [1], [2], [17]
  CpxR-Phosphorylated activator ppiDp2 Sigma70 -165.0 -261.0 ppiD
ccgcagaccgGTAAAGAGATCACCAtcgctgctgc
 461647 461661 [HIBSCS], [SM] [18]
  CpxR-Phosphorylated activator ppiDp2 Sigma70 -117.0 -213.0 ppiD
ttccgtgcagGTAAAGCACTGAAAGacgcggtaaa
 461695 461709 [HIBSCS], [SM] [18]
  CpxR-Phosphorylated activator ppiDp2 Sigma70 -96.5 -192.0 ppiD
gaaagacgcgGTAAACTAAGCGTTGTccccagtggg
 461715 461731 [HIBSCS], [SM] [18]
  CpxR-Phosphorylated repressor psdp Sigma24 151.0 -128.0 psd, mscM
cagaaaagttTTTACGCGTTTTTACctgcgccatg
 4390481 4390495 [ICA] [5]
  CpxR-Phosphorylated repressor rpoEp1a nd 67.0 44.0 rseD, rpoE, rseA, rseB, rseC
tctcgtttttGTAAAGTTCCGTAACgcatctgttt
 2710113 2710127 [AIBSCS], [GEA] [5]
  CpxR-Phosphorylated activator rpoHp1 Sigma70, Sigma38 nd 3600783.0 rpoH
nd
 nd nd [GEA] [19]
  CpxR-Phosphorylated activator rprAp nd nd -1770372.0 rprA
nd
 nd nd [BPP], [GEA] [11]
  CpxR-Phosphorylated activator sbmAp Sigma24 nd -396639.0 sbmA, yaiW
nd
 nd nd [AIBSCS], [GEA] [7]
  CpxR-Phosphorylated activator sltp7 Sigma32 -52.0 -84.5 slt
aaaagtgtccGTAACGTGGCGTAAAcggcaatgac
 4630641 4630656 [AIBSCS], [BPP], [GEA], [HIBSCS], [IGI] [7], [14]
  CpxR-Phosphorylated activator spyp Sigma70 -50.0 -113.0 spy
cagagcaacgGTAAAACAATGAAAGtgtaaaaaac
 1825731 1825745 [BPP], [GEA], [HIBSCS] [1], [7], [20], [21]
  CpxR-Phosphorylated activator srkAp Sigma70 -49.0 -73.0 srkA, dsbA
taattaaagaGTAAAAGCTTGTAAGcggcgccacc
 4042335 4042349 [BPP], [GEA], [HIBSCS] [1], [2], [22]
  CpxR-Phosphorylated activator tomBp1 Sigma70 -50.0 -136.0 tomB, hha
tcatcgcattATTACGAAAAATTACaattaggaag
 480837 480851 [BPP], [HIBSCS] [1]
  CpxR-Phosphorylated repressor tsrp1 Sigma70 -9.0 -254.0 tsr
ttacgcgcgcGTAAAGTAAGGTAAAtaactgagtg
 4591396 4591410 [BPP] [6]
  CpxR-Phosphorylated repressor ungp nd -81.0 -97.0 ung
attttaccaaTTTACATTTTTTTGCactcgtttaa
 2716650 2716664 [BCE], [BPP] [23]
  CpxR-Phosphorylated activator yccAp Sigma70 -53.0 -78.0 yccA
acccagaagtTTTACCCATCTTTACgcatttgatc
 1031489 1031503 [BPP], [GEA], [HIBSCS] [1], [7]
  CpxR-Phosphorylated activator ycfSp Sigma70 -53.5 -97.0 ldtC
gcagggaagtGCAAAGAATTTGTAAAtgttgcagat
 1170463 1170479 [BPP], [HIBSCS] [1]
  CpxR-Phosphorylated activator yebEp Sigma70 -44.0 -69.0 yebE
gtcagaaaatGTATATCGAGACAAAagatgcgcaa
 1929769 1929783 [BPP], [GEA], [HIBSCS] [1], [7]
  CpxR-Phosphorylated activator yidQp Sigma24 -254.0 -437.5 yidQ
tagcaatgcgGTAATGGTTTTCGTCTAccagttcaac
 3867282 3867299 [GEA], [HIBSCS] [13]
  CpxR-Phosphorylated activator yidQp Sigma24 -176.0 -359.5 yidQ
tttctaagtgGTTAAACAAACGGTCAAatccaatagc
 3867360 3867377 [GEA], [HIBSCS] [13]
  CpxR-Phosphorylated activator yidQp Sigma24 -143.5 -327.0 yidQ
tagcagaacgGTAAAGCGGGGATAAAtcaaagttac
 3867393 3867409 [GEA], [HIBSCS] [13]
  CpxR-Phosphorylated activator yqaEp nd -48.0 -105.5 yqaE, kbp
agcttacgctTTTACTCTCATTTACgctgctgagg
 2797126 2797141 [AIBSCS], [BPP], [GEA], [HIBSCS] [7], [11], [14]
  CpxR-Phosphorylated activator yqjAp1 Sigma70 -47.0 -272.0 yqjA, mzrA
agtataaagtGTCAGCCTGTGTAAAtcctctcgcc
 3247494 3247508 [BPP], [HIBSCS] [1]



High-throughput Transcription factor binding sites (TFBSs)
      

  Functional conformation Function Object name Object type Distance to first Gene Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  CpxR-Phosphorylated activator yjfN nd nd
nd
 nd nd [ICWHO] nd


Alignment and PSSM for CpxR TFBSs    

Aligned TFBS of CpxR   
  Sequence
  TGACGGTAATTTAC
  TTGTTCGTCTTTAC
  TTACCAGGTTTTAC
  GGTGATCTCTTTAC
  TTTCAGTGCTTTAC
  CAACGCTTAGTTTA
  TTACGAAAAATTAC
  AAACGGGGTTTTAC
  TTTTTCTTTTTGAA
  TTACTTTTGGTTAC
  TTACGGAATATTAC
  TATCTGTTTGTTAA
  TTACCCATCTTTAC
  TTTTATAAACATTC
  TTACATCTGATTTC
  TTACAAATCTTAAA
  AGTTACATGTTTAA
  TATTAAAATGTTAA
  TTTTATTAAGTTTA
  TTTTCTATCATTTC
  TTGGTTTTTATTGC
  TTGCACAATTTTAA
  TGGTGATAACTTAC
  ATATGCATTATTAG
  GGACAATTTTTTAC
  TAACGCTGCGTTAC
  TTACAAATTCTTTG
  TGACTTATACTTGC
  TTTCATAGAAATCC
  TTTCATTGTTTTAC
  TTGTCTCGATATAC
  TTACGTCTTTTTAC
  TTATATATTTTTAC
  TAACGCGTAGATAA
  AGACGATTCCAGAC
  ATTTACATTTTGAA
  TTATTTCGCCATTC
  TTACCCTTCTTTAC
  TTACATTAACTGAC
  TGACCAAAGAATAC
  ATGCCCTTTTATAC
  TTACGGAACTTTAC
  TTACATTTTTTTGC
  TTACGGCGTTTTGC
  TTAGGTGAAGAGGC
  TTTGTTGCCTTTAC
  TTTCGCCGCTTTAC
  TTACACAGGCTGAC
  TTACCTAATTTTAC
  CGGTAATGGTTTTC
  TGACCGTTTGTTTA
  TATCCCCGCTTTAC
  TTACAAGCTTTTAC
  TTACGTTGTTTTAC
  TGACGCATGTTTGC
  TATCGTTGAATCGC
  TTACGCGTTTTTAC
  TTACCTTACTTTAC

Position weight matrix (PWM).    
A	6	8	35	0	20	11	20	14	13	9	9	1	41	11
C	2	0	0	40	11	17	8	2	15	8	0	1	1	45
G	2	11	7	4	19	8	8	15	7	9	0	6	7	2
T	48	39	16	14	8	22	22	27	23	32	49	50	9	0

PWM logo   


 


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
TF-target gene evolutionary conservation
Promoter-target gene evolutionary conservation


Evidence    

 [BPP] Binding of purified proteins

 [IPI] Inferred from physical interaction

 [GEA] Gene expression analysis

 [IC] Inferred by curator

 [HIBSCS] Human inference based on similarity to consensus sequences

 [ICA] Inferred by computational analysis

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [SM] Site mutation

 [IGI] Inferred from genetic interaction

 [BCE] Binding of cellular extracts

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Yamamoto K., Ishihama A., 2006, Characterization of copper-inducible promoters regulated by CpxA/CpxR in Escherichia coli., Biosci Biotechnol Biochem 70(7):1688-95

 [2] Pogliano J., Lynch AS., Belin D., Lin EC., Beckwith J., 1997, Regulation of Escherichia coli cell envelope proteins involved in protein folding and degradation by the Cpx two-component system., Genes Dev 11(9):1169-82

 [3] Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A., 2005, Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli., J Biol Chem 280(2):1448-56

 [4] Hirakawa H., Inazumi Y., Masaki T., Hirata T., Yamaguchi A., 2005, Indole induces the expression of multidrug exporter genes in Escherichia coli., Mol Microbiol 55(4):1113-26

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 [15] Weatherspoon-Griffin N., Yang D., Kong W., Hua Z., Shi Y., 2014, The CpxR/CpxA two-component regulatory system up-regulates the multidrug resistance cascade to facilitate Escherichia coli resistance to a model antimicrobial peptide., J Biol Chem 289(47):32571-82

 [16] Batchelor E., Walthers D., Kenney LJ., Goulian M., 2005, The Escherichia coli CpxA-CpxR envelope stress response system regulates expression of the porins ompF and ompC., J Bacteriol 187(16):5723-31

 [17] Oshima T., Aiba H., Masuda Y., Kanaya S., Sugiura M., Wanner BL., Mori H., Mizuno T., 2002, Transcriptome analysis of all two-component regulatory system mutants of Escherichia coli K-12., Mol Microbiol 46(1):281-91

 [18] Dartigalongue C., Raina S., 1998, A new heat-shock gene, ppiD, encodes a peptidyl-prolyl isomerase required for folding of outer membrane proteins in Escherichia coli., EMBO J 17(14):3968-80

 [19] Zahrl D., Wagner M., Bischof K., Koraimann G., 2006, Expression and assembly of a functional type IV secretion system elicit extracytoplasmic and cytoplasmic stress responses in Escherichia coli., J Bacteriol 188(18):6611-21

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 [22] Danese PN., Silhavy TJ., 1997, The sigma(E) and the Cpx signal transduction systems control the synthesis of periplasmic protein-folding enzymes in Escherichia coli., Genes Dev 11(9):1183-93

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 [31] Isaac DD., Pinkner JS., Hultgren SJ., Silhavy TJ., 2005, The extracytoplasmic adaptor protein CpxP is degraded with substrate by DegP., Proc Natl Acad Sci U S A 102(49):17775-9

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 [33] Bachhawat P., Stock AM., 2007, Crystal structures of the receiver domain of the response regulator PhoP from Escherichia coli in the absence and presence of the phosphoryl analog beryllofluoride., J Bacteriol 189(16):5987-95


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