RegulonDB RegulonDB 10.8:Regulon Page
   

Nac DNA-binding transcriptional dual regulator

Synonyms: Nac
Summary:
Nac, "Nitrogen assimilation control," regulates, without a coeffector, genes involved in nitrogen metabolism under nitrogen-limiting conditions [1] The genes regulated by Nac are transcribed by RNA polymerase σ70. These genes are coupled to the nitrogen regulatory (Ntr) system, which is σ54 dependent, through Nac, whose transcription is activated by NtrC [1] Using DNA microarray analyses, it was shown that Nac could affect the expression of 25 genes [2] This transcriptional regulator is negatively autoregulated [1]and is expressed under nitrogen-limiting conditions [7] Nac belongs to the LysR family [1]and features a helix-turn-helix motif located close to the N terminus [] In contrast with most LysR family members, which are transcribed divergently from the gene they regulate, nac is distant on the genome from its regulated genes [1] In Klebsiella pneumoniae, Nac functions as a dimer, and for some sites it can bind as a tetramer; it recognizes long and short sites in the absence of a coeffector [] Review: [11] Read more >


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
Nac Functional   [HIFS], [IDA], [IFC] [1], [2]
Evolutionary Family: LysR
Connectivity class: Local Regulator
Gene name: nac
  Genome position: 2061016-2061933
  Length: 918 bp / 305 aa
Operon name: nac
TU(s) encoding the TF:
Transcription unit        Promoter
nac
nacp


Regulon       
Regulated gene(s) asnC, codA, codB, feaR, gabD, gabP, gabT, gdhA, gltB, gltD, gltF, mioC, mnmG, nac, nupC, patD, serA, ydcS, ydcT, ydcU, ydcV
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
membrane (5)
nitrogen metabolism (4)
Transcription related (3)
activator (3)
nucleotide and nucleoside conversions (3)
Read more >
Regulated operon(s) asnC-mioC-mnmG-rsmG, codBA, feaR, gdhA, glaH-lhgD-gabDTP, gltBDF, nac, nupC, serA, ydcSTUV-patD
First gene in the operon(s) asnC, asnC, asnC, codB, feaR, gabD, gdhA, gltB, nac, nupC, serA, ydcS
Simple and complex regulons AdiY,ArgR,CRP,FNR,Fur,GadE,HdfR,IHF,Lrp,Nac
ArgP,CRP,FNR,Fur,Nac
AsnC,Nac
CRP,CytR,Nac
CRP,Nac,NsrR,PhoB,QseB
Read more >
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[Nac,-](5)
[Nac,+](5)


Transcription factor regulation    


Transcription factor binding sites (TFBSs) arrangements
      

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  Nac repressor asnCp Sigma70 -64.0 -92.0 asnC, mioC, mnmG
aaaccgacacACATGCAATAATCATtcaataaaaa
 3927088 3927102 [APIORCISFBSCS], [CV(GEA)], [GEA] [3]
  Nac repressor asnCp Sigma70 -7.0 -35.0 asnC, mioC, mnmG
taaaaaagaaGGCTAAAATAGAATGaatcatcaat
 3927031 3927045 [APIORCISFBSCS], [CV(GEA)], [GEA] [3]
  Nac repressor asnCp Sigma70 16.0 -13.0 asnC, mioC, mnmG
atgaatcatcAATCCGCATAAGAAAatcctatgga
 3927009 3927023 [APIORCISFBSCS], [CV(GEA)], [GEA] [3]
  Nac activator codBp Sigma70 -58.0 -96.0 codB, codA
gcactcattcATATAAAAAATATATttccccacga
 354819 354833 [AIBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] [2], [4]
  Nac activator feaRp2 Sigma70 -32.5 -58.5 feaR
gtcaggctcaTTAGCTTCGTTTATTgcaacacaaa
 1447334 1447348 [IHBCE] [5]
  Nac activator gabDp1 Sigma70, Sigma38 -44.0 -145.0 gabD, gabT, gabP
cggcagcgacATCAGCAATTCCTATtggtgcgcat
 2791121 2791135 [APIORCISFBSCS], [CV(GEA)], [GEA] [2], [6]
  Nac repressor gdhAp1 Sigma70 -81.0 -144.0 gdhA
tcgcatttggTTATGAGATTACTCTcgttattaat
 1842220 1842234 [APIORCISFBSCS], [CV(GEA)], [GEA] [7]
  Nac repressor gdhAp1 Sigma70 -25.0 -88.0 gdhA
ttcttgcttaCCGTCACATTCTTGAtggtatagtc
 1842276 1842290 [APIORCISFBSCS], [CV(GEA)], [GEA] [7]
  Nac repressor gdhAp1 Sigma70 25.0 -39.0 gdhA
cacatgacatAAACAACATAAGCACaatcgtatta
 1842325 1842339 [APIORCISFBSCS], [CV(GEA)], [GEA] [7]
  Nac repressor gdhAp1 Sigma70 45.0 -19.0 gdhA
agcacaatcgTATTAATATATAAGGgttttatatc
 1842345 1842359 [APIORCISFBSCS], [CV(GEA)], [GEA] [7]
  Nac repressor gltBp Sigma38, Sigma70 nd nd gltB, gltD, gltF
nd
 nd nd [GEA] [2]
  Nac repressor nacp Sigma54 -75.0 -120.0 nac
tgacgaatctATCATAATCTCTTATttgacagttg
 2062046 2062060 [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [1]
  Nac activator nupCp Sigma70 nd nd nupC
nd
 nd nd [GEA] [2]
  Nac repressor serAp1 Sigma70 -30.0 -76.0 serA
gcaaacgttcATATTGCCGCAATATtattttttga
 3058479 3058493 [APIORCISFBSCS], [CV(GEA)], [GEA] [8]
  Nac repressor serAp1 Sigma70 -19.0 -65.0 serA
tattgccgcaATATTATTTTTTGATatgttgaaag
 3058468 3058482 [APIORCISFBSCS], [CV(GEA)], [GEA] [8]
  Nac activator ydcSp1 Sigma38 -51.0 -106.0 ydcS, ydcT, ydcU, ydcV, patD
cacaacctgaATAAATTTTCTCAGGggcgaaggtg
 1511541 1511555 [APIORCISFBSCS], [CV(GEA)], [CV(GEA)], [GEA], [IDA] [9]



High-throughput Transcription factor binding sites (TFBSs)
      

  Functional conformation Function Object name Object type Distance to first Gene Sequence LeftPos RightPos Center Position Growth Condition Evidence (Confirmed, Strong, Weak) References
  Nac activator nd tu nd
tatcgaaaccCATAGCAAATAATGATTcaacaacgca
2385814 2385831 2385822.0 nd , , [10]
  Nac activator nd tu nd
tgatgacacgCATACTCTCTCCTTATAaccaattgtt
997510 997527 997518.0 nd , , [10]
  Nac activator ivy tu nd
agaataaattGATAACAAATGCTGATAttggaaatat
240267 240284 240275.0 nd , , [10]
  Nac activator nd tu nd
tcttgttaatTATAAGGATATTTTATGcgctataatg
4283073 4283090 4283081.0 nd , , [10]
  Nac activator ackA tu nd
cagccaatggCATAATGTATATGAATTtgacgactca
2413286 2413303 2413294.0 nd , , [10]
  Nac activator yqeK tu nd
tcttcagaaaTATCCGCTATCTTTATTgacaaatcat
2990733 2990750 2990741.0 nd , , [10]
  Nac activator nd tu nd
tccataaatgCAAAAGTATTTTGTATGcgtggtttta
820717 820734 820725.0 nd , , [10]
  Nac activator ybiN tu nd
atttttacaaAATAAGACAAATCAATGacatgcataa
842176 842193 842184.0 nd , , [10]
  Nac activator nd tu nd
ttagccaaaaAATAAGAAAAGATTATGcaagcaacag
3674772 3674789 3674780.0 nd , , [10]
  Nac activator yagU tu nd
ctaatataaaAATAGGGAGAAATGATGgagcttatat
302848 302865 302856.0 nd , , [10]
  Nac activator ygjH tu nd
gtacgcgtcgTATAAAAAGATCGGATGgcgacgtcgt
3220903 3220920 3220911.0 nd , , [10]
  Nac activator nd tu nd
atattcattaATAAAAGCCATACTTATagcacaaagg
569295 569312 569303.0 nd , , [10]
  Nac activator nd tu nd
ttacccgaatCATAAGATTCTGATATCttaaataaga
3584539 3584556 3584547.0 nd , , [10]
  Nac activator nd tu nd
aaaacgccaaCATAAGAGAAACCTATGccattacccg
607381 607398 607389.0 nd , , [10]
  Nac activator yjaH tu nd
ataaacacatTGTAAGGATAACTTATGaacaagactc
4200267 4200284 4200275.0 nd , , [10]
  Nac activator yaiS tu nd
aatatcaataCATATAATAAAACTATTatattcgcat
384977 384994 384985.0 nd , , [10]
  Nac activator nd tu nd
aaactcaacgCTATAAGGCGTTGTTCTggttatcgtt
3609243 3609260 3609251.0 nd , , [10]
  Nac activator nd tu nd
ttaattacagCATAACGATTAATGATAcacgtacagg
3113132 3113149 3113140.0 nd , , [10]
  Nac activator rfaH tu nd
tcgctccatgCTATTGCTTAATCTTATtaataaagag
4024385 4024402 4024393.0 nd , , [10]
  Nac activator ybeQ tu nd
aaatggaacaCATAAAAATATAATATGttttatctgc
676576 676593 676584.0 nd , , [10]
  Nac activator rlmM tu nd
gcgggttatgTCATTTATGAATGTTATcaacctgatg
2941103 2941120 2941111.0 nd , , [10]
  Nac activator ydeE tu nd
ggttttattgCCATTACTTTAGTGAATaacgtgacgc
1621597 1621614 1621605.0 nd , , [10]
  Nac activator dcp tu nd
ctccgttatcAGTAAGCCTACGCTATGgaacacctcg
1627407 1627424 1627415.0 nd , , [10]
  Nac activator nd tu nd
ttactcacgaTGATACGGTTTTGTTATtaagaggaac
1212942 1212959 1212950.0 nd , , [10]
  Nac activator nd tu nd
aaagggagaaTAAACGTCTTACTTATAgaacagtgaa
4436611 4436628 4436619.0 nd , , [10]
  Nac activator ybhD tu nd
ttaccgctggCAATCGGTAAATTTAATgagctgtatc
800228 800245 800236.0 nd , , [10]
  Nac activator nd tu nd
gcgaaattccCATAACTAATTAGGATTattacaaaaa
1256267 1256284 1256275.0 nd , , [10]
  Nac activator nd tu nd
gcttaccactTATAAAGATTAATTATAaatatataat
2249633 2249650 2249641.0 nd , , [10]
  Nac activator nd tu nd
tctgacacaaGATAAGCAAAAGTGATTtatgtgatta
3354195 3354212 3354203.0 nd , , [10]
  Nac activator nd tu nd
aggcgacctgCCAAAAGCCAAAGTTATccacgggcga
2476418 2476435 2476426.0 nd , , [10]
  Nac activator ycaK tu nd
tgctaaaaggATATATTGACAGAGTATggaataatgg
949967 949984 949975.0 nd , , [10]
  Nac activator nd tu nd
ttttggcagtAATAATAAAATATGATGtttccttgcc
3321211 3321228 3321219.0 nd , , [10]
  Nac activator nd tu nd
tgccggtgacGATCGTTATTTTTAATAatggcggcat
2490531 2490548 2490539.0 nd , , [10]
  Nac activator yjgM tu nd
tgtctgtataTATAAGTTATATCAATGgattttttaa
4479689 4479706 4479697.0 nd , , [10]
  Nac activator uspC tu nd
gtgattgattAATTATCTTTACTAATAatcagactaa
1979632 1979649 1979640.0 nd , , [10]
  Nac activator ycaN tu nd
cccattattcCATACTCTGTCAATATAtccttttagc
949968 949985 949976.0 nd , , [10]
  Nac activator bisC tu nd
caacataaacGCATTCGTGAGGCTTATggtccggcat
3716080 3716097 3716088.0 nd , , [10]
  Nac activator wecH tu nd
cttaagcctcTATAAGCTGTAGTGATAaaactcagat
3725795 3725812 3725803.0 nd , , [10]
  Nac activator nd tu nd
gatatgaataAATTGAAATATCTTATGacgcttctta
246141 246158 246149.0 nd , , [10]
  Nac activator nd tu nd
cttcatgccaTCATAGAGAAAATAAATaaccgcatcg
1770808 1770825 1770816.0 nd , , [10]
  Nac activator ybeT tu nd
tgaattaaatCATCACGATTATTTATTccccaggatg
679645 679662 679653.0 nd , , [10]
  Nac repressor nd tu nd
tcttcagaaaTATCCGCTATCTTTATTgacaaatcat
2990733 2990750 2990741.0 nd , , [10]
  Nac repressor nd tu nd
gatgccggacCATAAGCCTCACGAATGcgtttatgtt
3716079 3716096 3716087.0 nd , , [10]
  Nac repressor ddlA tu nd
caacctttatTATAAATAAAAGTTATGaaatatattc
401177 401194 401185.0 nd , , [10]
  Nac repressor eptA tu nd
ttgtcctgacACTAAGCTCTTTCTTATggcttaatcg
4335374 4335391 4335382.0 nd , , [10]
  Nac repressor nd tu nd
gattttaaatGAATAAGAAATCGTTATggaagctaat
801889 801906 801897.0 nd , , [10]
  Nac repressor nd tu nd
attgtgattgGCATAGATAATACTTATccgacaatta
3066865 3066882 3066873.0 nd , , [10]
  Nac repressor nd tu nd
gagactttttTATTGGATTTACTTATAcggcaacgat
3896693 3896710 3896701.0 nd , , [10]
  Nac repressor yafC tu nd
ttcaacgagcAATTGTATATTGTTATGtaagcaagtg
231015 231032 231023.0 nd , , [10]
  Nac repressor nd tu nd
gtattggctgTCAGGAATTATTTTTCTtgcctatgga
2192762 2192779 2192770.0 nd , , [10]
  Nac repressor nd tu nd
gggtgataatACATCCAATTTTTCTCTtattgtcgat
3897634 3897651 3897642.0 nd , , [10]
  Nac repressor nd tu nd
aactggttggATATACAAGATTCTTTTgatgcttctg
2044048 2044065 2044056.0 nd , , [GEA] [10]
  Nac repressor nd tu nd
cactacttaaCATTAAATTAACTTATAcaattcagtt
1678206 1678223 1678214.0 nd , , [10]
  Nac repressor nd tu nd
acgatttgtcCATAAATGATTATTATCgtaaagttaa
234215 234232 234223.0 nd , , [10]
  Nac repressor abgR tu nd
ttcacgtagcGATAGTTTTTACTTATCactaactgat
1404611 1404628 1404619.0 nd , , [10]
  Nac repressor yebQ tu nd
attcaccaaaGATATAAAATTCCTATGccaaaagttc
1910262 1910279 1910270.0 nd , , [10]
  Nac repressor nd tu nd
tcttcgccaaCTATCTGCTTTTCTTCTggatcttctg
3674084 3674101 3674092.0 nd , , [10]
  Nac repressor eamA tu nd
ggttttattgCCATTACTTTAGTGAATaacgtgacgc
1621597 1621614 1621605.0 nd , , [10]
  Nac repressor nd tu nd
catatattcaCATTAAATATGATTATGtacttgttac
3287383 3287400 3287391.0 nd , , [10]
  Nac repressor nd tu nd
ttagtgtgctTATCTTTTCTTCTTATGttcaccgcgc
1115525 1115542 1115533.0 nd , , [10]
  Nac repressor nd tu nd
cgtgctgatgGACAACCTATTTTTATGgccgcgatag
2010188 2010205 2010196.0 nd , , [10]
  Nac repressor nd tu nd
atattcgtccCATAAGGAGTTTTTCTTgattacacat
2926293 2926310 2926301.0 nd , , [10]
  Nac repressor yghT tu nd
ttacctgaacCATCACATCCTACAATGgcaataacac
3133888 3133905 3133896.0 nd , , [10]
  Nac repressor nd tu nd
tcacaggtgtTCGTCAAATTGAGTTATggcggcgtga
4086069 4086086 4086077.0 nd , , [10]
  Nac repressor nd tu nd
caacaaaataGATTAACCAACCTAATGaaaaacaaat
2892069 2892086 2892077.0 nd , , [10]
  Nac repressor nd tu nd
atgaacaaatCATTCGCCATGATTATAatatttatcc
641899 641916 641907.0 nd , , [10]
  Nac repressor nd tu nd
aaaatctttcCATAACAAATGGTTATTcattaatcct
4294254 4294271 4294262.0 nd , , [10]
  Nac repressor nd tu nd
atagcctatgAATAAGCTAACGCTATGatgtccgtgg
4428762 4428779 4428770.0 nd , , [10]
  Nac repressor nd tu nd
ccagcaaataCATTAGACAAAAATATGggcatcatgg
3632989 3633006 3632997.0 nd , , [10]
  Nac repressor nd tu nd
tctgccgttcCATAAAGCAAACCAATTcctcggtatg
3172983 3173000 3172991.0 nd , , [10]
  Nac repressor nd tu nd
ctttcgttaaCATAAGGAGTGCATTATgcgcatagct
2215642 2215659 2215650.0 nd , , [10]
  Nac repressor nd tu nd
agcctggtgtAATAACAATTCCCGATAgtcttttgct
2859653 2859670 2859661.0 nd , [10]
  Nac repressor nd tu nd
gcttaccactTATAAAGATTAATTATAaatatataat
2249633 2249650 2249641.0 nd , , [10]
  Nac repressor nd tu nd
ggtatttgtcCGTATTAAAAAATTATGttggtttctc
3252928 3252945 3252936.0 nd , , [10]
  Nac repressor nd tu nd
taatataaatTATATATAAATCTTATTtatgtgatag
1996895 1996912 1996903.0 nd , , [10]
  Nac repressor nd tu nd
tgccttcctcGGATACGAATATATTATgacgccggtt
3226582 3226599 3226590.0 nd , , [10]
  Nac repressor yfbK tu nd
tatcgaaaccCATAGCAAATAATGATTcaacaacgca
2385814 2385831 2385822.0 nd , , [10]
  Nac repressor nd tu nd
cagagtgcgcTATAAATATTCACAATAgggcgcgaat
3333499 3333516 3333507.0 nd , , [10]
  Nac repressor nd tu nd
ctgaagtaagAATTATCCTAGGGGATGctgtagtgga
1633125 1633142 1633133.0 nd , , [10]
  Nac repressor nd tu nd
tcacgagtgcTATCATCCGCCCTTATGcttagcgcat
1357697 1357714 1357705.0 nd , , [10]
  Nac repressor nd tu nd
gcgtcattgcCCATATGAACGAATTATtaatcgccct
1517710 1517727 1517718.0 nd , , [10]
  Nac repressor nd tu nd
tgaagcatagTGATAAGCTTTTCGTCTtacgagtccc
216619 216636 216627.0 nd , , [10]
  Nac repressor nd tu nd
ctgaagtaagAATTATCCTAGGGGATGctgtagtgga
1434155 1434172 1434163.0 nd , , [10]
  Nac repressor nd tu nd
tttttttatcCATTCGAACTCTTTATActggttactc
4297158 4297175 4297166.0 nd , , [10]
  Nac repressor nd tu nd
aaatgacgggGATAACCATATTTTATGgcgataacat
3267262 3267279 3267270.0 nd , , [10]
  Nac repressor nd tu nd
tatgtcgcccTATAACTATACATGATGtcaataagag
1655690 1655707 1655698.0 nd , , [10]
  Nac repressor nd tu nd
ttacaattcaCATAATTTGTCATTATTcattccgcaa
3418272 3418289 3418280.0 nd , , [10]
  Nac repressor nd tu nd
ttgcgatgcgCATCAGTAAACGCTATCaacacctggt
1919888 1919905 1919896.0 nd , , [10]
  Nac repressor ycaC tu nd
atctcttcagCATTAGCGTTATGAATGagtaatataa
945739 945756 945747.0 nd , , [10]
  Nac repressor yjgN tu nd
tgtctgtataTATAAGTTATATCAATGgattttttaa
4479689 4479706 4479697.0 nd , , [10]
  Nac repressor nd tu nd
atgaacagtcCATCGCTAATTATTATTggtcgggttg
3499134 3499151 3499142.0 nd , , [10]
  Nac repressor nd tu nd
ttcatgccatCATAGAGAAAATAAATAaccgcatcgg
1770809 1770826 1770817.0 nd , , [10]
  Nac repressor nd tu nd
atctcttcagCATTAGCGTTATGAATGagtaatataa
945739 945756 945747.0 nd , , [10]
  Nac repressor nd tu nd
tcaaataagtCCTAAGTTTTAAATATAtcaaaaatta
850405 850422 850413.0 nd , , [10]
  Nac repressor nd tu nd
cttctcaggcGAGAAGCCGTTCTTATTaccggacaat
2897893 2897910 2897901.0 nd , , [10]
  Nac repressor nd tu nd
ccgcttactcAAGAAGAAAGAATTATGacgttgtctc
4609400 4609417 4609408.0 nd , , [10]
  Nac repressor nd tu nd
acgtggcccgGTATCACTTTTCTTAATgactctggct
2759168 2759185 2759176.0 nd , , [10]
  Nac repressor nudG tu nd
gttgttgccgCCATCATTGAACGTGATggcaaaattt
1841515 1841532 1841523.0 nd , , [10]
  Nac repressor nd tu nd
acttaataaaTATCAGCTATTCTTATAaagaaaatct
2099737 2099754 2099745.0 nd , , [10]
  Nac repressor nd tu nd
gcgtcattgcCCATATGAACGAATTATtaatcgccct
1517710 1517727 1517718.0 nd , , [10]
  Nac repressor yaiY tu nd
cgtaattgttAATAAATTTAACTTAAGgttgccattt
399494 399511 399502.0 nd , , [10]
  Nac repressor nd tu nd
agatacgtacCATCATTTCCTCCTATAggctgatttc
1806356 1806373 1806364.0 nd [AIBSCS], , [10]
  Nac repressor ykgL tu nd
tttttgataaGAAGAAAAAGACATTTGgtttcataag
311568 311585 311576.0 nd , , [10]
  Nac repressor ligB tu nd
ataaagcctaCATACGAGCTTATTATAcacggcgcgg
3821374 3821391 3821382.0 nd , , [10]
  Nac repressor nd tu nd
acggcgtggtTATAAATTTCCGCAATGgcagcacagt
1518792 1518809 1518800.0 nd , , [10]
  Nac repressor nd tu nd
ttgcccgttgCATAACCTTTAATTATTcatatgcatt
4374417 4374434 4374425.0 nd , , [10]
  Nac repressor nd tu nd
tggcatgataAATAGGTATAAAGAATGggaaatcgga
1755139 1755156 1755147.0 nd , , [10]
  Nac repressor ycgJ tu nd
acggttggatTATCAGGATGTGCTATGaatatgatgc
1226586 1226603 1226594.0 nd , , [10]
  Nac repressor nd tu nd
ttcatggctaTCATCCCAAAAAACTATgcgcggttag
430612 430629 430620.0 nd , , [10]
  Nac repressor dld tu nd
tcctactgaaCATAGCCATTTCATATGgatttttctt
2221976 2221993 2221984.0 nd , , [10]
  Nac repressor nd tu nd
agcccatgggCATATCATCCCTTTATAgagaaaacga
2984192 2984209 2984200.0 nd , , [10]
  Nac repressor nd tu nd
ggtgataataCATCCAATTTTTCTCTTattgtcgata
3897635 3897652 3897643.0 nd , , [10]
  Nac repressor nd tu nd
atttatattgCCATCACGTTCTGGTATtgggtgcgct
1053207 1053224 1053215.0 nd , , [10]
  Nac repressor yjeH tu nd
tcatcgccgtCATAAGCGAATCTGATTgtgctaccat
4370473 4370490 4370481.0 nd , , [10]
  Nac repressor nd tu nd
agactattatCATAGGTAGGCCAAATGaggataaccg
2971243 2971260 2971251.0 nd , , [10]
  Nac repressor nd tu nd
gtttgaaggcTTATTAGTTATCGATATgacacatgtc
2489116 2489133 2489124.0 nd , , [10]
  Nac repressor kdgR tu nd
attcaccaaaGATATAAAATTCCTATGccaaaagttc
1910262 1910279 1910270.0 nd , , [10]
  Nac repressor nd tu nd
gttttgatacGCTAGCGTAGCCTTATAgatctaaggt
2089156 2089173 2089164.0 nd , , [10]
  Nac repressor nd tu nd
cagccaatggCATAATGTATATGAATTtgacgactca
2413286 2413303 2413294.0 nd , , [10]
  Nac repressor nd tu nd
ctacccgctgGATTGGTAACCCGTATAagaaaaacgc
4148594 4148611 4148602.0 nd , , [10]
  Nac repressor nd tu nd
tgattctgttGATATGATCACGTTATAcccaatgtgc
3536753 3536770 3536761.0 nd , , [10]
  Nac repressor nd tu nd
accatatcgcAATAAGTTATGTTTTTAaattgagggc
800726 800743 800734.0 nd , , [10]


Alignment and PSSM for Nac TFBSs    

Aligned TFBS of Nac   
  Sequence
  GGATTTTCTTATGCGGA
  AAATTTTCTCAGGGGCG
  GGAATTGCTGATGTCGC
  TTATTTTTTGATATGTT
  ATAATCTCTTATTTGAC
  TATATTTTTTATATGAA
  TGATTATTGCATGTGTG
  AGCTTCGTTTATTGCAA
  GAGTAATCTCATAACCA
  TCATTCTATTTTAGCCT
  GATTGTGCTTATGTTGT
  AAATAATATTGCGGCAA
  GTATTAATATATAAGGG
  TCACATTCTTGATGGTA

Position weight matrix (PWM). Nac matrix-quality result    
A	4	5	10	3	3	4	1	2	1	0	11	1	5	2	0	4	6
C	0	2	1	1	0	3	0	7	0	3	0	1	0	1	5	3	2
G	5	4	1	0	1	0	3	0	1	2	2	1	6	5	8	4	3
T	5	3	2	10	10	7	10	5	12	9	1	11	3	6	1	3	3

Consensus    
;	consensus.strict             	ggattttcTtatgggga
;	consensus.strict.rc          	TCCCCATAAGAAAATCC
;	consensus.IUPAC              	krattykyTyatrkssr
;	consensus.IUPAC.rc           	YSSMYATRARMRAATYM
;	consensus.regexp             	[gt][ag]att[ct][gt][ct]T[ct]at[ag][gt][cg][cg][ag]
;	consensus.regexp.rc          	[CT][CG][CG][AC][CT]AT[AG]A[AG][AC][AG]AAT[CT][AC]

PWM logo   


 


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
TF-target gene evolutionary conservation
Promoter-target gene evolutionary conservation


Evidence    

 [HIFS] Human inference of function from sequence

 [IDA] Inferred from direct assay

 [IFC] Inferred by functional complementation

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [CV(GEA)] cross validation(GEA)

 [GEA] Gene expression analysis

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [BPP] Binding of purified proteins

 [CV(GEA/SM)] cross validation(GEA/SM)

 [CV(SM)] cross validation(SM)

 [SM] Site mutation

 [IHBCE] Inferred by a human based on computational evidence

 [AIBSPD] Automated inference based on sequence pattern discovery

 [CE] ChIP-seq evidence

 [RE] RNA-seq evidence

 [MCIBSPD] Manually curated inference based on sequence pattern discovery


Reference(s)    

 [1] Muse WB., Bender RA., 1998, The nac (nitrogen assimilation control) gene from Escherichia coli., J Bacteriol 180(5):1166-73

 [2] Zimmer DP., Soupene E., Lee HL., Wendisch VF., Khodursky AB., Peter BJ., Bender RA., Kustu S., 2000, Nitrogen regulatory protein C-controlled genes of Escherichia coli: scavenging as a defense against nitrogen limitation., Proc Natl Acad Sci U S A 97(26):14674-9

 [3] Poggio S., Domeinzain C., Osorio A., Camarena L., 2002, The nitrogen assimilation control (Nac) protein represses asnC and asnA transcription in Escherichia coli., FEMS Microbiol Lett 206(2):151-6

 [4] Muse WB., Rosario CJ., Bender RA., 2003, Nitrogen regulation of the codBA (cytosine deaminase) operon from Escherichia coli by the nitrogen assimilation control protein, NAC., J Bacteriol 185(9):2920-6

 [5] Zeng J., Spiro S., 2013, Finely tuned regulation of the aromatic amine degradation pathway in Escherichia coli., J Bacteriol 195(22):5141-50

 [6] Schneider BL., Ruback S., Kiupakis AK., Kasbarian H., Pybus C., Reitzer L., 2002, The Escherichia coli gabDTPC operon: specific gamma-aminobutyrate catabolism and nonspecific induction., J Bacteriol 184(24):6976-86

 [7] Camarena L., Poggio S., Garcia N., Osorio A., 1998, Transcriptional repression of gdhA in Escherichia coli is mediated by the Nac protein., FEMS Microbiol Lett 167(1):51-6

 [8] Blauwkamp TA., Ninfa AJ., 2002, Nac-mediated repression of the serA promoter of Escherichia coli., Mol Microbiol 45(2):351-63

 [9] Schneider BL., Hernandez VJ., Reitzer L., 2013, Putrescine catabolism is a metabolic response to several stresses in Escherichia coli., Mol Microbiol 88(3):537-50

 [10] Aquino P., Honda B., Jaini S., Lyubetskaya A., Hosur K., Chiu JG., Ekladious I., Hu D., Jin L., Sayeg MK., Stettner AI., Wang J., Wong BG., Wong WS., Alexander SL., Ba C., Bensussen SI., Bernstein DB., Braff D., Cha S., Cheng DI., Cho JH., Chou K., Chuang J., Gastler DE., Grasso DJ., Greifenberger JS., Guo C., Hawes AK., Israni DV., Jain SR., Kim J., Lei J., Li H., Li D., Li Q., Mancuso CP., Mao N., Masud SF., Meisel CL., Mi J., Nykyforchyn CS., Park M., Peterson HM., Ramirez AK., Reynolds DS., Rim NG., Saffie JC., Su H., Su WR., Su Y., Sun M., Thommes MM., Tu T., Varongchayakul N., Wagner TE., Weinberg BH., Yang R., Yaroslavsky A., Yoon C., Zhao Y., Zollinger AJ., Stringer AM., Foster JW., Wade J., Raman S., Broude N., Wong WW., Galagan JE., 2017, Coordinated regulation of acid resistance in Escherichia coli., BMC Syst Biol 11(1):1

 [11] Switzer A, Brown DR, Wigneshweraraj S, 2018, New insights into the adaptive transcriptional response to nitrogen starvation in Escherichia coli., Biochem Soc Trans, 2018 Dec 17


RegulonDB