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RcsB DNA-binding transcriptional dual regulator

Synonyms: RcsB, RcsB-Pasp56
Summary:
RcsB protein for "Regulator capsule synthesis B," is a response regulator that belongs to the multicomponent RcsF/RcsC/RcsD/RcsA-RcsB phosphorelay system [4, 13, 14, 15, 16] and is involved in the regulation of the synthesis of colanic acid capsule, cell division, periplasmic proteins, motility, biofilm formation, and a small RNA [2, 13, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26]. The response regulator RcsB is the principal regulator of this system and is capable真of真forming真complexes with the真RcsA [13, 16, 19, 24, 25, 27, 28, 29] and MatA,真DctR, and真BglJ真proteins真(forming a heterodimer) [29], while RcsB activates the others genes independently (in the form of a homodimer) [1, 2, 8, 9, 11, 13, 29]. RcsC is a sensor histidine kinase and is known to be a transmembrane protein composed of three domains: the external sensory domain (amino terminal), a cytoplasmic transmitter domain (carboxyl terminal), and a transmembrane hydrophobic central domain (unknown function).
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Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
RcsB Non-Functional   Apo nd nd
RcsB-Pasp56 Functional Covalent Holo nd nd
Evolutionary Family: LuxR/UhpA
Sensing class: External-Two-component systems
Connectivity class: Local Regulator
Gene name: bglJ
  Genome position: 4604160-4604837
  Length: 678 bp / 225 aa
Operon name: yjjQ-bglJ
TU(s) encoding the TF:
Transcription unit        Promoter
yjjQ-bglJ
yjjQp
Gene name: gadE
  Genome position: 3658366-3658893
  Length: 528 bp / 175 aa
Operon name: gadEF-mdtEF
TU(s) encoding the TF:
Transcription unit        Promoter
gadEF
gadEp1
gadEF
gadEp3
gadEF-mdtEF
gadEp
gadEF-mdtEF
gadEp4
Gene name: rcsA
  Genome position: 2023968-2024591
  Length: 624 bp / 207 aa
Operon name: rcsA
TU(s) encoding the TF:
Transcription unit        Promoter
rcsA
rcsAp
Gene name: rcsB
  Genome position: 2316177-2316827
  Length: 651 bp / 216 aa
Operon name: rcsDB
TU(s) encoding the TF:
Transcription unit        Promoter
rcsB
rcsBp1
rcsB
rcsBp2
rcsDB
rcsDp


Regulon       
Regulated gene(s) bdm, ftsA, ftsZ, gadA, gadB, gadC, gadX, gadY, hdeA, hdeB, hdeD, lolA, osmB, osmC, rarA, rpoE, rprA, rseA, rseB, rseC, rseD, safA, sra, ydeO, ydeP, yhiD
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
pH (6)
Transcription related (6)
membrane (4)
sigma factors, anti-sigmafactors (4)
other (mechanical, nutritional, oxidative stress) (4)
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Regulated operon(s) bdm-sra, gadAXW, gadBC, gadY, hdeAB-yhiD, hdeD, lolA-rarA, mraZ-rsmH-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ-lpxC, osmB, osmC, rprA, rseD-rpoE-rseABC, safA-ydeO, ydeP
First gene in the operon(s) bdm, ftsA, gadA, gadB, gadY, hdeA, hdeD, lolA, osmB, osmC, rprA, rseD, safA, ydeP
Simple and complex regulons AdiY,ArcA,CRP,FNR,Fis,GadE-RcsB,GadW,GadX,H-NS,RcsB,TorR
AdiY,CRP,Fis,FliZ,GadE,GadW,GadX,RcsB
CRP,GlrR,NtrC,RcsB
CpxR,RcsB
EvgA,H-NS,NagC,NarL,NarP,PhoP,RcsB,UvrY
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Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[RcsB-BglJ,+](21)
[RcsB,-](1)


Transcription factor regulation    


Transcription factor binding sites (TFBSs) arrangements
      

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  RcsB-Pasp56 activator bdmp nd -41.5 -101.5 bdm, sra
gcttcgttgtTAAGATTAGTCCTGgttgatgatt
1556375 1556388 [BPP], [GEA] [1]
  RcsB-Pasp56 activator ftsAp1 Sigma70 -43.5 -464.5 ftsA, ftsZ
ctgatgaattGAAGATTCATCTGGttgaatatgt
103511 103524 [GEA], [HIBSCS], [SM] [2]
  RcsB-Pasp56 repressor gadAp Sigma38, Sigma70, Sigma38, Sigma70 -18.5 -45.5 gadA, gadX
aataaaaaagTAGGATTTATCCGCaatggaagca
3667619 3667632 [BPP], [GEA], [IHBCE], [SM] [3], [4], [5]
  RcsB-Pasp56 activator gadBp Sigma38, Sigma70, Sigma38, Sigma70 -64.5 -92.0 gadB, gadC
tcgtgtttaaATAACAAAATCCTAatgttattta
1572130 1572144 [GEA], [HIBSCS] [4], [6]
  RcsB-Pasp56 activator gadYp Sigma38 nd -3664864.0 gadY nd nd [GEA] [6]
  RcsB-Pasp56 activator hdeAp Sigma38, Sigma70, Sigma70, Sigma38 nd 3656740.0 hdeA, hdeB, yhiD nd nd [GEA] [6]
  RcsB-Pasp56 activator hdeDp Sigma70 nd -3656995.0 hdeD nd nd [GEA] [6]
  RcsB-Pasp56 activator lolAp Sigma70 -43.5 -87.5 lolA, rarA
aactgaaaatTCAGTATTTTCTTCtttcctcaag
937278 937291 [GEA], [HIBSCS] [7]
  RcsB-Pasp56 activator osmBp1 Sigma38 -41.5 -189.5 osmB
gttaataacaGGTAAATAGTCTTAattatcaacc
1343511 1343524 [GEA], [HIBSCS] [8]
  RcsB-Pasp56 activator osmCp1 Sigma70 -41.5 -70.5 osmC
cggattttatTCGGAATATCCTGCttatcctcgt
1556548 1556561 [BPP], [GEA], [SM] [1], [9]
  RcsB-Pasp56 activator rpoEp2b Sigma70, Sigma54 -52.5 -227.5 rseD, rpoE, rseA, rseB, rseC
gatgctatgcTGACCAAACCATGTttagtaaatt
2710385 2710398 [BPP], [HIBSCS], [SM] [10]
  RcsB-Pasp56 activator rprAp nd -42.5 -42.5 rprA
tttaaactaaTGAGACGAATCTGAtcgacgcaaa
1770323 1770336 [GEA], [HIBSCS], [SM] [11]
  RcsB-Pasp56 activator safAp Sigma70 nd 1583959.0 safA, ydeO nd nd [GEA] [6]
  RcsB-Pasp56 activator ydePp Sigma70 nd 1586486.0 ydeP nd nd [BPP], [GEA] [6], [12]


Alignment and PSSM for RcsB TFBSs    

Aligned TFBS of RcsB   
  Sequence
  AACCAGATGAATCT
  ATTCAGTATTTTCT
  ATTAAGACTATTTA
  ACCAGGACTAATCT
  ATTCGGAATATCCT
  ATTAGGATTTTGTT
  AATGAGACGAATCT
  AGTAGGATTTATCC

Position weight matrix (PWM).   
A	8	2	0	4	4	0	7	2	0	5	4	0	0	1
C	0	1	2	3	0	0	0	3	0	0	0	1	6	1
G	0	1	0	1	4	8	0	0	2	0	0	1	0	0
T	0	4	6	0	0	0	1	3	6	3	4	6	2	6

PWM logo   


 


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
TF-target gene evolutionary conservation
Promoter-target gene evolutionary conservation


Evidence    

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [HIBSCS] Human inference based on similarity to consensus sequences

 [SM] Site mutation

 [IHBCE] Inferred by a human based on computational evidence



Reference(s)    

 [1] Francez-Charlot A., Castanie-Cornet MP., Gutierrez C., Cam K., 2005, Osmotic Regulation of the Escherichia coli bdm (Biofilm-Dependent Modulation) Gene by the RcsCDB His-Asp Phosphorelay., J Bacteriol 187(11):3873-7

 [2] Carballes F., Bertrand C., Bouche JP., Cam K., 1999, Regulation of Escherichia coli cell division genes ftsA and ftsZ by the two-component system rcsC-rcsB., Mol Microbiol 34(3):442-50

 [3] Castanie-Cornet MP., Cam K., Bastiat B., Cros A., Bordes P., Gutierrez C., 2010, Acid stress response in Escherichia coli: mechanism of regulation of gadA transcription by RcsB and GadE., Nucleic Acids Res 38(11):3546-54

 [4] Castanie-Cornet MP., Treffandier H., Francez-Charlot A., Gutierrez C., Cam K., 2007, The glutamate-dependent acid resistance system in Escherichia coli: essential and dual role of the His-Asp phosphorelay RcsCDB/AF., Microbiology 153(Pt 1):238-46

 [5] Krin E., Danchin A., Soutourina O., 2010, RcsB plays a central role in H-NS-dependent regulation of motility and acid stress resistance in Escherichia coli., Res Microbiol 161(5):363-371

 [6] Johnson MD., Burton NA., Gutierrez B., Painter K., Lund PA., 2011, RcsB Is Required for Inducible Acid Resistance in Escherichia coli and Acts at gadE-Dependent and -Independent Promoters., J Bacteriol 193(14):3653-6

 [7] Tao K., Narita S., Tokuda H., 2012, Defective lipoprotein sorting induces lolA expression through the Rcs stress response phosphorelay system., J Bacteriol 194(14):3643-50

 [8] Boulanger A., Francez-Charlot A., Conter A., Castanie-Cornet MP., Cam K., Gutierrez C., 2005, Multistress regulation in Escherichia coli: expression of osmB involves two independent promoters responding either to sigmaS or to the RcsCDB His-Asp phosphorelay., J Bacteriol 187(9):3282-6

 [9] Davalos-Garcia M., Conter A., Toesca I., Gutierrez C., Cam K., 2001, Regulation of osmC gene expression by the two-component system rcsB-rcsC in Escherichia coli., J Bacteriol 183(20):5870-6

 [10] Klein G., Stupak A., Biernacka D., Wojtkiewicz P., Lindner B., Raina S., 2016, Multiple Transcriptional Factors Regulate Transcription of the rpoE Gene in Escherichia coli under Different Growth Conditions and When the Lipopolysaccharide Biosynthesis Is Defective., J Biol Chem 291(44):22999-23019

 [11] Majdalani N., Hernandez D., Gottesman S., 2002, Regulation and mode of action of the second small RNA activator of RpoS translation, RprA., Mol Microbiol 46(3):813-26

 [12] Yoshida M., Ishihama A., Yamamoto K., 2015, Cross talk in promoter recognition between six NarL-family response regulators of Escherichia coli two-component system., Genes Cells 20(7):601-12

 [13] Majdalani N., Gottesman S., 2005, The Rcs phosphorelay: a complex signal transduction system., Annu Rev Microbiol 59:379-405

 [14] Majdalani N., Heck M., Stout V., Gottesman S., 2005, Role of RcsF in signaling to the Rcs phosphorelay pathway in Escherichia coli., J Bacteriol 187(19):6770-8

 [15] Castanie-Cornet MP., Cam K., Jacq A., 2006, RcsF is an outer membrane lipoprotein involved in the RcsCDB phosphorelay signaling pathway in Escherichia coli., J Bacteriol 188(12):4264-70

 [16] Gottesman S., Stout V., 1991, Regulation of capsular polysaccharide synthesis in Escherichia coli K12., Mol Microbiol 5(7):1599-606

 [17] Huang YH., Ferrieres L., Clarke DJ., 2006, The role of the Rcs phosphorelay in Enterobacteriaceae., Res Microbiol 157(3):206-12

 [18] Jayaratne P., Keenleyside WJ., MacLachlan PR., Dodgson C., Whitfield C., 1993, Characterization of rcsB and rcsC from Escherichia coli O9:K30:H12 and examination of the role of the rcs regulatory system in expression of group I capsular polysaccharides., J Bacteriol 175(17):5384-94

 [19] Wehland M., Bernhard F., 2000, The RcsAB box. Characterization of a new operator essential for the regulation of exopolysaccharide biosynthesis in enteric bacteria., J Biol Chem 275(10):7013-20

 [20] Sledjeski DD., Gottesman S., 1996, Osmotic shock induction of capsule synthesis in Escherichia coli K-12., J Bacteriol 178(4):1204-6

 [21] Gottesman S., Trisler P., Torres-Cabassa A., 1985, Regulation of capsular polysaccharide synthesis in Escherichia coli K-12: characterization of three regulatory genes., J Bacteriol 162(3):1111-9

 [22] Stout V., Torres-Cabassa A., Maurizi MR., Gutnick D., Gottesman S., 1991, RcsA, an unstable positive regulator of capsular polysaccharide synthesis., J Bacteriol 173(5):1738-47

 [23] Sledjeski D., Gottesman S., 1995, A small RNA acts as an antisilencer of the H-NS-silenced rcsA gene of Escherichia coli., Proc Natl Acad Sci U S A 92(6):2003-7

 [24] Stout V., 1996, Identification of the promoter region for the colanic acid polysaccharide biosynthetic genes in Escherichia coli K-12., J Bacteriol 178(14):4273-80

 [25] Francez-Charlot A., Laugel B., Van Gemert A., Dubarry N., Wiorowski F., Castani?-Cornet MP., Gutierrez C., Cam K., 2003, RcsCDB His-Asp phosphorelay system negatively regulates the flhDC operon in Escherichia coli., Mol Microbiol 49(3):823-32

 [26] Vianney A., Jubelin G., Renault S., Dorel C., Lejeune P., Lazzaroni JC., 2005, Escherichia coli tol and rcs genes participate in the complex network affecting curli synthesis., Microbiology 151(Pt 7):2487-97

 [27] Ogasawara H., Hasegawa A., Kanda E., Miki T., Yamamoto K., Ishihama A., 2007, Genomic SELEX search for target promoters under the control of the PhoQP-RstBA signal relay cascade., J Bacteriol 189(13):4791-9

 [28] Ferrieres L., Aslam SN., Cooper RM., Clarke DJ., 2007, The yjbEFGH locus in Escherichia coli K-12 is an operon encoding proteins involved in exopolysaccharide production., Microbiology 153(Pt 4):1070-80

 [29] Pannen D., Fabisch M., Gausling L., Schnetz K., 2016, Interaction of the RcsB Response Regulator with Auxiliary Transcription Regulators in Escherichia coli., J Biol Chem 291(5):2357-70

 [30] Mouslim C., Latifi T., Groisman EA., 2003, Signal-dependent requirement for the co-activator protein RcsA in transcription of the RcsB-regulated ugd gene., J Biol Chem 278(50):50588-95

 [31] Schmoe K., Rogov VV., Rogova NY., Lohr F., Guntert P., Bernhard F., Dotsch V., 2011, Structural insights into Rcs phosphotransfer: the newly identified RcsD-ABL domain enhances interaction with the response regulator RcsB., Structure 19(4):577-87

 [32] Lee YY., Chang CF., Kuo CL., Chen MC., Yu CH., Lin PI., Wu WF., 2003, Subunit oligomerization and substrate recognition of the Escherichia coli ClpYQ (HslUV) protease implicated by in vivo protein-protein interactions in the yeast two-hybrid system., J Bacteriol 185(8):2393-401

 [33] Hirakawa H., Nishino K., Hirata T., Yamaguchi A., 2003, Comprehensive studies of drug resistance mediated by overexpression of response regulators of two-component signal transduction systems in Escherichia coli., J Bacteriol 185(6):1851-6



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