RegulonDB RegulonDB 10.9:Regulon Page

FhlA DNA-binding transcriptional activator

Synonyms: FhlA-formate, FhlA
The transcription factor FhlA, for "Formate hydrogen lyase Activator," is positively autoregulated and controls the transcription of the operons involved in formate metabolism to produce dihydrogen and carbon dioxide [2, 3, 8, 9, 10] Transcription of this operon is induced when E. coli is grown under anaerobic growth conditions and when the physiological inducer, formate, binds to the FhlA protein [2, 8, 10] When formate binds to FhlA, the protein becomes active as the binding affinity of FhlA increases, and it activates the transcription of σ54-dependent promoters. HycA may inactivate the function of the FhlA protein and prevent the binding of FhlA to target sequences, but the mechanism of the negative regulation by HycA is unknown [11] FhlA is subject to posttranscriptional regulation by the sRNA OxyS [12, 13] This sRNA binds to FhlA mRNA and inhibits translation by interfering with the binding of the mRNA and the ribosome [12, 13, 14] As a member of the group of σ54-dependent transcriptional regulators, FhlA contains three domains: a possible sensory domain located in the N terminus that probably modulates the activity of the C-terminal domain [] the central domain, which is probably involved in open complex formation, ATP hydrolysis, and interaction with σ54 and formate [] and the C-terminal domain, which contains a helix-turn-helix motif for DNA binding [1] FhlA shows 46% identity to the HyfR protein, a member of the same group.
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Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
FhlA Non-Functional   Apo [BPP], [GEA] [1]
FhlA-formate Functional Allosteric Holo [APPH], [BPP], [GEA], [IFC] [1], [2]
Evolutionary Family: EBP
Sensing class: Using internal synthesized signals
Connectivity class: Local Regulator
Gene name: fhlA
  Genome position: 2854338-2856416
  Length: 2079 bp / 692 aa
Operon name: hypABCDE-fhlA
TU(s) encoding the TF:
Transcription unit        Promoter

Regulated gene(s) fdhF, fhlA, focB, hycA, hycB, hycC, hycD, hycE, hycF, hycG, hycH, hycI, hydN, hyfA, hyfB, hyfC, hyfD, hyfE, hyfF, hyfG, hyfH, hyfI, hyfJ, hyfR, hypA, hypB, hypC, hypD, hypE, hypF
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
anaerobic respiration (16)
fermentation (11)
membrane (9)
posttranslational modification (4)
operon (3)
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Regulated operon(s) fdhF, hycABCDEFGHI, hydN-hypF, hyfABCDEFGHIJR-focB, hypABCDE-fhlA
First gene in the operon(s) fdhF, hycA, hydN, hyfA, hypA
Simple and complex regulons CRP,FNR,FhlA,HyfR
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)

Transcription factor regulation    

Transcription factor binding sites (TFBSs) arrangements

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  FhlA-formate activator fdhFp Sigma54 -129.5 -170.5 fdhF
4299530 4299543 [APIORCISFBSCS], [BPP], [GEA] [2]
  FhlA-formate activator fdhFp Sigma54 -104.5 -145.5 fdhF
4299505 4299518 [APIORCISFBSCS], [BPP], [GEA] [2]
  FhlA-formate activator hycAp Sigma54 -98.0 -124.0 hycA, hycB, hycC, hycD, hycE, hycF, hycG, hycH, hycI
2850553 2850566 [APIORCISFBSCS], [BPP], [GEA] [1], [2], [3], [4]
  FhlA-formate activator hycAp Sigma54 -84.5 -110.5 hycA, hycB, hycC, hycD, hycE, hycF, hycG, hycH, hycI
2850539 2850552 [APIORCISFBSCS], [BPP], [GEA] [1], [2], [3]
  FhlA-formate activator hydNp Sigma54 nd nd hydN, hypF nd nd [BPP], [GEA], [IGI] [5]
  FhlA-formate activator hyfAp Sigma54 -130.0 -160.0 hyfA, hyfB, hyfC, hyfD, hyfE, hyfF, hyfG, hyfH, hyfI, hyfJ, hyfR, focB
2601034 2601047 [GEA] [6], [7]
  FhlA-formate activator hypAp Sigma54 -751.5 -771.5 hypA, hypB, hypC, hypD, hypE, fhlA
2849869 2849882 [APIORCISFBSCS], [BPP], [GEA] [1], [2], [3]
  FhlA-formate activator hypAp Sigma54 -720.5 -740.5 hypA, hypB, hypC, hypD, hypE, fhlA
2849900 2849913 [APIORCISFBSCS], [BPP], [GEA] [1], [2], [3]

Alignment and PSSM for FhlA TFBSs    

Aligned TFBS of FhlA   

Position weight matrix (PWM). FhlA matrix-quality result   
A	3	0	1	0	0	3	1	0	0	1	0	0	5	1	2	1	2
C	1	2	1	1	6	3	0	0	1	0	5	0	2	5	0	1	1
G	1	1	4	3	0	0	4	0	2	0	0	7	0	1	5	1	3
T	2	4	1	3	1	1	2	7	4	6	2	0	0	0	0	4	1

;	consensus.strict             	atggCcgTttCGaCGtg
;	consensus.strict.rc          	CACGTCGAAACGGCCAT
;	consensus.IUPAC              	aygkCmgTktCGmCGtg
;	consensus.IUPAC.rc           	CACGKCGAMACKGMCRT
;	consensus.regexp             	a[ct]g[gt]C[ac]gT[gt]tCG[ac]CGtg
;	consensus.regexp.rc          	CACG[GT]CGA[AC]AC[GT]G[AC]C[AG]T

PWM logo   


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
TF-target gene evolutionary conservation
Promoter-target gene evolutionary conservation


 [1] Leonhartsberger S., Ehrenreich A., Bock A., 2000, Analysis of the domain structure and the DNA binding site of the transcriptional activator FhlA., Eur J Biochem 267(12):3672-84

 [2] Schlensog V., Lutz S., Bock A., 1994, Purification and DNA-binding properties of FHLA, the transcriptional activator of the formate hydrogenlyase system from Escherichia coli., J Biol Chem 269(30):19590-6

 [3] Hopper S., Babst M., Schlensog V., Fischer HM., Hennecke H., Bock A., 1994, Regulated expression in vitro of genes coding for formate hydrogenlyase components of Escherichia coli., J Biol Chem 269(30):19597-604

 [4] Self WT., Shanmugam KT., 2000, Isolation and characterization of mutated Fh1A proteins which activate transcription of the hyc operon (formate hydrogenlyase) of Escherichia coli in the absence of molybdate(1)., FEMS Microbiol Lett 184(1):47-52

 [5] Maier T., Binder U., Bock A., 1996, Analysis of the hydA locus of Escherichia coli: two genes (hydN and hypF) involved in formate and hydrogen metabolism., Arch Microbiol 165(5):333-41

 [6] Self WT., Hasona A., Shanmugam KT., 2004, Expression and regulation of a silent operon, hyf, coding for hydrogenase 4 isoenzyme in Escherichia coli., J Bacteriol 186(2):580-7

 [7] Skibinski DA., Golby P., Chang YS., Sargent F., Hoffman R., Harper R., Guest JR., Attwood MM., Berks BC., Andrews SC., 2002, Regulation of the hydrogenase-4 operon of Escherichia coli by the sigma(54)-dependent transcriptional activators FhlA and HyfR., J Bacteriol 184(23):6642-53

 [8] Maupin JA., Shanmugam KT., 1990, Genetic regulation of formate hydrogenlyase of Escherichia coli: role of the fhlA gene product as a transcriptional activator for a new regulatory gene, fhlB., J Bacteriol 172(9):4798-806

 [9] Schlensog V., Bock A., 1990, Identification and sequence analysis of the gene encoding the transcriptional activator of the formate hydrogenlyase system of Escherichia coli., Mol Microbiol 4(8):1319-27

 [10] Iuchi S., Lin EC., 1993, Adaptation of Escherichia coli to redox environments by gene expression., Mol Microbiol 9(1):9-15

 [11] Sauter M., Bohm R., Bock A., 1992, Mutational analysis of the operon (hyc) determining hydrogenase 3 formation in Escherichia coli., Mol Microbiol 6(11):1523-32

 [12] Argaman L., Altuvia S., 2000, fhlA repression by OxyS RNA: kissing complex formation at two sites results in a stable antisense-target RNA complex., J Mol Biol 300(5):1101-12

 [13] Altuvia S., Zhang A., Argaman L., Tiwari A., Storz G., 1998, The Escherichia coli OxyS regulatory RNA represses fhlA translation by blocking ribosome binding., EMBO J 17(20):6069-75

 [14] Altuvia S., Weinstein-Fischer D., Zhang A., Postow L., Storz G., 1997, A small, stable RNA induced by oxidative stress: role as a pleiotropic regulator and antimutator., Cell 90(1):43-53

 [15] Andrews SC., Berks BC., McClay J., Ambler A., Quail MA., Golby P., Guest JR., 1997, A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-translocating formate hydrogenlyase system., Microbiology 143 ( Pt 11):3633-47