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GlcC DNA-binding transcriptional dual regulator

Synonyms: GlcC-glycolate, GlcC-Acetate, GlcC
Summary:
GlcC (for "glycolate utilization") negatively controls the expression of genes involved in the utilization of glycolate as the sole source of carbon [1, 2] It is negatively autoregulated and coordinately activated by transcription of the divergent operon glc, which is related to the transport and metabolism of glycolate [1] Synthesis of glc genes is induced when Escherichia coli is grown on glycolate [1] GlcC features an N-terminal domain containing a helix-turn-helix motif [2]and a putative C-terminal domain that includes the key residues involved in coinducer recognition and oligomerization. Although little is known about the mechanism of regulation of the GlcC transcription factor, Pellicer et al. demonstrated that it is a regulator that acts as a repressor by binding to cis-acting elements, inverted repeat sequences that are not well-conserved, that overlap the ATG sequence [1] Read more >


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
GlcC Non-Functional   Apo [BPP] [1]
GlcC-Acetate Functional Allosteric Holo [BPP] [1]
GlcC-glycolate Functional Allosteric Holo [APPH], [APPPINH], [BPP], [GEA], [HIFS], [IEP] [1], [2]
Evolutionary Family: GntR
Sensing class: Sensing external and internal signals
Connectivity class: Local Regulator
Gene name: glcC
  Genome position: 3128272-3129036
  Length: 765 bp / 254 aa
Operon name: glcC
TU(s) encoding the TF:
Transcription unit        Promoter
glcC
glcCp


Regulon       
Regulated gene(s) glcA, glcB, glcC, glcD, glcE, glcF, glcG
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
carbon compounds (3)
glycolate metabolism (3)
Porters (Uni-, Sym- and Antiporters) (1)
membrane (1)
glyoxylate degradation (1)
Read more >
Regulated operon(s) glcC, glcDEFGBA
First gene in the operon(s) glcC, glcD
Simple and complex regulons ArcA,GlcC,IHF,PdhR
CRP,Cra,Fis,GlcC
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[GlcC,-](1)
[GlcC,+](1)


Transcription factor regulation    


Transcription factor binding sites (TFBSs) arrangements
      

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  GlcC-glycolate repressor glcCp Sigma70 47.0 -20.0 glcC
ccggacctcgTGCACAGGTAGGACCaatttttctg
3128245 3128259 [BCE], [GEA], [SM] [1]
  GlcC-glycolate activator glcDp Sigma70 -176.0 -231.0 glcD, glcE, glcF, glcG, glcB, glcA
cagaaaaattGGTCCTACCTGTGCAcgaggtccgg
3128245 3128259 [BCE], [GEA], [SM] [1]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [BPP] Binding of purified proteins

 [APPH] Assay of protein purified to homogeneity

 [APPPINH] Assay of protein partially-purified from its native host

 [GEA] Gene expression analysis

 [HIFS] Human inference of function from sequence

 [IEP] Inferred from expression pattern

 [BCE] Binding of cellular extracts

 [SM] Site mutation



Reference(s)    

 [1] Pellicer MT., Fernandez C., Badia J., Aguilar J., Lin EC., Baldom L., 1999, Cross-induction of glc and ace operons of Escherichia coli attributable to pathway intersection. Characterization of the glc promoter., J Biol Chem 274(3):1745-52

 [2] Pellicer MT., Badia J., Aguilar J., Baldoma L., 1996, glc locus of Escherichia coli: characterization of genes encoding the subunits of glycolate oxidase and the glc regulator protein., J Bacteriol 178(7):2051-9



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