|Synonyms: MhpR-3HPP, MhpR-3-(2,3-dihydroxyphenyl)propionate [DHPP], MhpR|
MhpR, hydroxyphenylpropionate regulator, participates in controlling the expression of several genes involved in degradation of phenylpropionate (PP) |CITS:|. MhpR is able to bind to its DNA-binding site even in the absence of inducer; however, increasing concentrations of inducer improve the binding strength of MhpR. 3-(2,3-Dihydroxyphenyl)propionate (DHPP) and 3-hydroxyphenylpropionate (3HPP), two intermediates of the metabolic pathways, are the principal inducers of MhpR. The third inducer, phenylpropionate (PP), acts synergistically with DHPP and 3HPP |CITS:|.
This protein, which belongs to the IclR family of transcriptional regulators, has a helix-turn-helix motif to bind DNA in its N-terminal domain and a 22-residue motif, specific to an IclR subfamily, in its C-terminal domain |CITS:|.
MhpR is necessary to recruit CRP to the regulatory region of the mhp operon, where both proteins activate transcription |CITS:|.
The mhpR gene, which is located downstream of the genes it regulates and in the opposite direction, appears to be expressed constitutively |CITS:|.
|Sensing class:||Sensing external and internal signals|
|Connectivity class:||Local Regulator|
|Length:||834 bp / 277 aa|
|TU(s) encoding the TF:||
|Regulated gene(s)||mhpA, mhpB, mhpC, mhpD, mhpE, mhpF|
|Multifun term(s) of regulated gene(s)||
MultiFun Term (List of genes associated to the multifun term)
carbon compounds (5)
mhpA, mhpC, mhpD, mhpE, mhpF
fatty acids (1)
|First gene in the operon(s)||mhpA|
|Simple and complex regulons|
|Simple and complex regulatory phrases||
Regulatory phrase (List of promoters regulated by the phrase)
|Functional conformation||Function||Promoter||Sigma factor||Central Rel-Pos||Distance to first Gene||Genes||Sequence||LeftPos||RightPos||Evidence (Confirmed, Strong, Weak)||References|
|MhpR-3HPP||activator||mhpAp||Sigma70||-58.0||-150.0||mhpA, mhpB, mhpC, mhpD, mhpF, mhpE||
|368454||368468||[BCE], [GEA], [HIBSCS], [SM]|||
|Evolutionary conservation of regulatory elements|
 Manso I., Torres B., Andreu JM., Menendez M., Rivas G., Alfonso C., Diaz E., Garcia JL., Galan B., 2009, 3-Hydroxyphenylpropionate and phenylpropionate are synergistic activators of the MhpR transcriptional regulator from Escherichia coli., J Biol Chem. 284(32):21218-28