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EnvY DNA-binding transcriptional activator

Synonyms: EnvY
EnvY is a DNA-binding transcriptional regulator that participates in the control of several genes that encode cellular envelope proteins at low temperatures and during stationary phase [2] This protein is an envelope protein [2]that contains a putative membrane-spanning region, and as for the members of the family of transcriptional regulators (AraC/XylS) that it pertains to [4] it also contains a helix-turn-helix motif for DNA binding in the C-terminal region [4, 5] The transcriptional regulators of E. coli that show higher similarity to EnvR are AppY and AdiY [5] The latter, like EnvY, also has a putative transmembrane region [5] No research has been done to determine the DNA-binding sites for EnvY. The regulation of envY has not been described yet, but a region of dyad symmetry has been found upstream of this gene [6] EnvY: "envelope polypeptide" [2] Read more >

Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
EnvY Functional   [IE] [1]
Evolutionary Family: AraC/XylS
Connectivity class: Local Regulator
Gene name: envY
  Genome position: 586147-586908
  Length: 762 bp / 253 aa
Operon name: envY-ompT
TU(s) encoding the TF:
Transcription unit        Promoter

Regulated gene(s) ompC, ompF
Multifun term(s) of regulated gene(s)
Beta barrel porins (The Outer Membrane Porin (OMP) Functional Superfamily) (2)
Regulated operon(s) ompC, ompF
First gene in the operon(s) ompC, ompF
Simple and complex regulons CRP,CpxR,EnvY,Fur,IHF,OmpR,PhoB,RstA
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)

Transcription factor regulation    

Transcription factor binding sites (TFBSs) arrangements

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  EnvY activator ompCp1 Sigma70 nd nd ompC nd nd [IMP] [2], [3]
  EnvY activator ompFp Sigma38, Sigma70 nd nd ompF nd nd [IMP] [2]

Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


 [IE] Inferred from experiment

 [IMP] Inferred from mutant phenotype


 [1] Lundrigan MD., Friedrich MJ., Kadner RJ., 1989, Nucleotide sequence of the Escherichia coli porin thermoregulatory gene envY., Nucleic Acids Res 17(2):800

 [2] Lundrigan MD., Earhart CF., 1984, Gene envY of Escherichia coli K-12 affects thermoregulation of major porin expression., J Bacteriol 157(1):262-8

 [3] Otsuka J., Watanabe H., Mori KT., 1996, Evolution of transcriptional regulation system through promiscuous coupling of regulatory proteins with operons; suggestion from protein sequence similarities in Escherichia coli., J Theor Biol 178(2):183-204

 [4] Gallegos MT, Michán C, Ramos JL, 1993, The XylS/AraC family of regulators., Nucleic Acids Res, 1993 Feb 25

 [5] Lin JW, Yu KY, Chao YF, Weng SF, 1995, The lumQ gene is linked to the lumP gene and the lux operon in Photobacterium leiognathi., Biochem Biophys Res Commun, 1995 Dec 14

 [6] Stim-Herndon KP., Flores TM., Bennett GN., 1996, Molecular characterization of adiY, a regulatory gene which affects expression of the biodegradative acid-induced arginine decarboxylase gene (adiA) of Escherichia coli., Microbiology 142 ( Pt 5):1311-20