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SdiA DNA-binding transcriptional dual regulator

Synonyms: SdiA
The transcription factor SdiA, for Suppressor of the cell division inhibitor, is possibly positively autoregulated and controls the transcription of the genes involved in cell division |CITS:[8631660][8552633][11555297][1915297][11244066]|. SdiA has been shown to increase transcription from the P2 promoter of the ftsQAZ operon |CITS:[8552633]| by facilitating RNA polymerase binding to the promoter region |CITS:[11555297]|. SdiA activates the expression of ydiV, which is involved in the interaction between two quorum-sensing systems. An sdiA ydiV double mutant reduces cAMP levels, which inhibits quorum-sensing system 2 |CITS:[18560382]|. Expression of sdiAitself is regulated by a mechanism similar to quorum sensing: exposure to conditioned medium results in a 50-80% decrease in sdiA expression |CITS:[8631660]|. The transcriptional activity of SdiA is affected not only by quorum signaling but also by other environmental factors, such as oxidation |CITS:[24598739]|.
Overexpression of SdiA speeds up cell division |CITS:[1915297]| and causes apparently concomitant morphological changes; in both exponential and stationary phases, cells appear rounder and shorter |CITS:[11244065]|. Overexpression of SdiA also leads to resistance to the DNA-damaging agent mitomycin C as well as to other drugs |CITS:[11244065][11244066]|, while an sdiA null mutant strain was not hypersensitive to mitomycin C |CITS:[11244065]| but was more sensitive to flouroquinolones |CITS:[11929524]|.
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Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
SdiA     nd nd
Evolutionary Family: LuxR/UhpA
Sensing class: External sensing using transported metabolites
Connectivity class: Local Regulator
Gene name: sdiA
  Genome position: 1996110-1996832
  Length: 723 bp / 240 aa
Operon name: sdiA
TU(s) encoding the TF:
Transcription unit        Promoter

Regulated gene(s) ftsA, ftsQ, ftsZ, gadW, ydiV
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
cell division (3)
membrane (1)
Transcription related (1)
repressor (1)
posttranscriptional (1)
Regulated operon(s) gadAXW, mraZ-rsmH-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ-lpxC, ydiV
First gene in the operon(s) ftsQ, ftsQ, gadW, ydiV
Simple and complex regulons GadW,GadX,H-NS,PhoP,SdiA
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)

Transcription factor binding sites (TFBSs) arrangements       

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  SdiA repressor ftsQp1 Sigma38 -163.5 -451.5 ftsQ, ftsA, ftsZ
102695 102712 [BPP], [GEA] [1], [2]
  SdiA repressor ftsQp1 Sigma38 -161.5 -449.5 ftsQ, ftsA, ftsZ
102697 102714 [BPP], [GEA] [2]
  SdiA repressor ftsQp1 Sigma38 -15.5 -303.5 ftsQ, ftsA, ftsZ
102843 102860 [BPP] [2]
  SdiA activator ftsQp2 Sigma38 -36.5 -449.5 ftsQ
102697 102714 [BPP], [GEA] [2]
  SdiA activator gadWp2 nd nd nd gadW nd nd [GEA] [3]
  SdiA activator ydiVp Sigma24 -53.0 -120.0 ydiV
1792132 1792148 [AIBSCS], [BPP], [GEA] [4]

Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [AIBSCS] Automated inference based on similarity to consensus sequences


 [1] Wang XD., de Boer PA., Rothfield LI., 1991, A factor that positively regulates cell division by activating transcription of the major cluster of essential cell division genes of Escherichia coli., EMBO J. 10(11):3363-72

 [2] Yamamoto K., Yata K., Fujita N., Ishihama A., 2001, Novel mode of transcription regulation by SdiA, an Escherichia coli homologue of the quorum-sensing regulator., Mol Microbiol. 41(5):1187-98

 [3] Dyszel JL., Soares JA., Swearingen MC., Lindsay A., Smith JN., Ahmer BM., 2010, E. coli K-12 and EHEC genes regulated by SdiA., PLoS One. 5(1):e8946

 [4] Zhou X., Meng X., Sun B., 2008, An EAL domain protein and cyclic AMP contribute to the interaction between the two quorum sensing systems in Escherichia coli., Cell Res. 18(9):937-48