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Synonyms: SdiA |
Summary:
The transcription factor SdiA, for "Suppressor of the cell division inhibitor," is possibly positively autoregulated and controls the transcription of the genes involved in cell division [1, 2, 6, 7, 8] SdiA has been shown to increase transcription from the P2 promoter of the ftsQAZ operon [7]by facilitating RNA polymerase binding to the promoter region [2] SdiA activates the expression of ydiV, which is involved in the interaction between two quorum-sensing systems. An sdiA ydiV double mutant reduces cAMP levels, which inhibits quorum-sensing system 2 [5] Expression of sdiAitself is regulated by a mechanism similar to quorum sensing: exposure to conditioned medium results in a 50-80% decrease in sdiA expression [6] The transcriptional activity of SdiA is affected not only by quorum signaling but also by other environmental factors, such as oxidation [9] Overexpression of SdiA speeds up cell division [1]and causes apparently concomitant morphological changes; in both exponential and stationary phases, cells appear rounder and shorter [] Overexpression of SdiA also leads to resistance to the DNA-damaging agent mitomycin C as well as to other drugs [8] while an sdiA null mutant strain was not hypersensitive to mitomycin C []but was more sensitive to flouroquinolones [] In addition, SdiA also affects the expression of a number of genes, including a decrease in expression of several motility [3]and chemotaxis [8]genes, a pleiotropic effect over the expression of genes involved in cell division, drug sensitivity, DNA replication and repair, macromolecular metabolism [8] genes involved in the glutamate-dependent acid resistance systems (AR-2) [3] and an increase in the expression of the AcrAB multidrug efflux pump proteins [] Strains overexpressing SdiA had a chain-type cell morphology [] On the other hand, SdiA is highly homologous to quorum-sensing transcription factors that belong to the LuxR family [10] The molecule inducer is represented by different N-acyl-L-homoserine lactones (AHLs), which are signaling molecules involved in communication between bacteria (quorum-sensing systems) [3, 6, 9] AHLs might control the transcriptional activity of SdiA by enhancing its stability rather than by directly affecting its DNA-binding affinity [9] Lee et al. Read more > |
Transcription factor | ![]() ![]() |
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TF conformation(s): |
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Evolutionary Family: | LuxR/UhpA | ||||||||||||||||
Sensing class: | External sensing using transported metabolites | ||||||||||||||||
Connectivity class: | Local Regulator | ||||||||||||||||
Gene name: | sdiA | ||||||||||||||||
Genome position: | 1996110-1996832 | ||||||||||||||||
Length: | 723 bp / 240 aa | ||||||||||||||||
Operon name: | sdiA | ||||||||||||||||
TU(s) encoding the TF: |
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Regulon |
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Regulated gene(s) | ftsA, ftsQ, ftsZ, gadW, rmf, rsd, ydiV | ||||||
Multifun term(s) of regulated gene(s) |
MultiFun Term (List of genes associated to the multifun term)
cell division (3)
Transcription related (2)
membrane (1)
repressor (1)
translation (1)
Read more >
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Regulated operon(s) | gadAXW, mraZ-rsmH-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ-lpxC, rmf, rsd, ydiV | ||||||
First gene in the operon(s) | ftsQ, ftsQ, gadW, rmf, rsd, ydiV | ||||||
Simple and complex regulons | ArcA,CRP,McbR,RcdA,SdiA,SlyA,ppGpp ArcA,McbR,RcdA,SdiA,SlyA GadW,GadX,H-NS,PhoP,SdiA SdiA SdiA,ppGpp | ||||||
Simple and complex regulatory phrases | Regulatory phrase (List of promoters regulated by the phrase) |
Transcription factor regulation |
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Functional conformation | Function | Promoter | Sigma factor | Central Rel-Pos | Distance to first Gene | Genes | Sequence | LeftPos | RightPos | Evidence (Confirmed, Strong, Weak) | References | |
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SdiA | repressor | ftsQp1 | Sigma38, Sigma70 | -163.5 | -451.5 | ftsQ, ftsA, ftsZ |
tgtgggaatgTCAAAAGTAGTAGCAGAAaatgctctac
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102695 | 102712 | [BPP], [GEA] | [1], [2] | |
SdiA | repressor | ftsQp1 | Sigma38, Sigma70 | -161.5 | -449.5 | ftsQ, ftsA, ftsZ |
tgggaatgtcAAAAGTAGTAGCAGAAAAtgctctacaa
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102697 | 102714 | [BPP], [GEA] | [2] | |
SdiA | repressor | ftsQp1 | Sigma38, Sigma70 | -15.5 | -303.5 | ftsQ, ftsA, ftsZ |
atacgtattcAACCGTCCGGAACCTTCTatgattatga
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102843 | 102860 | [BPP] | [2] | |
SdiA | activator | ftsQp2 | Sigma38 | -36.5 | -449.5 | ftsQ |
tgggaatgtcAAAAGTAGTAGCAGAAAAtgctctacaa
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102697 | 102714 | [BPP], [GEA] | [2] | |
SdiA | activator | gadWp2 | nd | nd | nd | gadW | nd | nd | [GEA] | [3] | ||
SdiA | activator | rmfp | nd | nd | nd | rmf | nd | nd | [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/GS)], [CV(GEA/GS)], [GEA], [GS] | [4] | ||
SdiA | activator | rsdp2 | Sigma70 | nd | nd | rsd | nd | nd | [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/GS)], [CV(GEA/GS)], [GEA], [GS] | [4] | ||
SdiA | activator | ydiVp | Sigma24 | -53.0 | -120.0 | ydiV |
gataatctaaAAAAAGGACCCCTGAAAAagtggttaac
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1792132 | 1792149 | [AIBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] | [5] |
Evolutionary conservation of regulatory elements | ![]() |
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Reference(s) |
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