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ChbR DNA-binding transcriptional dual regulator

Synonyms: ChbR-Monoacetylchitobiose-6P, ChbR-N-monoacetylchitobiose-6-phosphate, ChbR


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
ChbR     nd nd
ChbR-N-monoacetylchitobiose-6-phosphate     nd nd
ChbR-Monoacetylchitobiose-6P Functional Allosteric Holo [GEA], [IPI], [SM] [1]
Evolutionary Family: AraC/XylS
Sensing class: External sensing using transported metabolites
Connectivity class: Local Regulator
Gene name: chbR
  Genome position: 1818605-1819447
  Length: 843 bp / 280 aa
Operon name: chbBCARFG
TU(s) encoding the TF:
Transcription unit        Promoter
chbBCARFG
chbBp


Regulon       
Regulated gene(s) chbA, chbB, chbC, chbF, chbG, chbR
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
carbon compounds (5)
Phosphotransferase Systems (PEP-dependent PTS) (3)
membrane (1)
polysaccharides (1)
Transcription related (1)
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Regulated operon(s) chbBCARFG
First gene in the operon(s) chbB
Simple and complex regulons CRP,ChbR,NagC
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[ChbR,+-](1)


Transcription factor binding sites (TFBSs) arrangements       

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  ChbR repressor chbBp nd -61.0 -168.0 chbB, chbC, chbA, chbR, chbF, chbG
tcataaccacAGGTCAAGGAGAAACAATTtataaggtca
1821778 1821796 [BPP], [GEA] [2], [3]
  ChbR-N-monoacetylchitobiose-6-phosphate activator chbBp nd -61.0 -168.0 chbB, chbC, chbA, chbR, chbF, chbG
tcataaccacAGGTCAAGGAGAAACAATTtataaggtca
1821778 1821796 [BPP], [GEA] [2], [3]
  ChbR repressor chbBp nd -38.0 -145.0 chbB, chbC, chbA, chbR, chbF, chbG
acaatttataAGGTCAAAGAAATACTATTgctcaggtct
1821755 1821773 [BPP], [GEA] [2], [3]
  ChbR-N-monoacetylchitobiose-6-phosphate activator chbBp nd -38.0 -145.0 chbB, chbC, chbA, chbR, chbF, chbG
acaatttataAGGTCAAAGAAATACTATTgctcaggtct
1821755 1821773 [BPP], [GEA] [2], [3]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [GEA] Gene expression analysis

 [IPI] Inferred from physical interaction

 [SM] Site mutation

 [BPP] Binding of purified proteins



Reference(s)    

 [1] Verma SC., Mahadevan S., 2012, The chbG Gene of the Chitobiose (chb) Operon of Escherichia coli Encodes a Chitooligosaccharide Deacetylase., J Bacteriol. 194(18):4959-71

 [2] Kachroo AH., Kancherla AK., Singh NS., Varshney U., Mahadevan S., 2007, Mutations that alter the regulation of the chb operon of Escherichia coli allow utilization of cellobiose., Mol Microbiol. 66(6):1382-95

 [3] Plumbridge J., Pellegrini O., 2004, Expression of the chitobiose operon of Escherichia coli is regulated by three transcription factors: NagC, ChbR and CAP., Mol Microbiol. 52(2):437-49



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