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UidR DNA-binding transcriptional repressor

Synonyms: UidR
Summary:
The β-d-glucuronides repressor, UidR, is a transcriptional repressor. UidR negatively regulates its own synthesis and represses transcription of the uid operon, which is involved in transport and degradation of β-glucosides [2, 3, 4, 5, 6]. This regulator is involved in the degradation of sugar acids and is subject to catabolic repression in the presence of glucose and at low levels of cyclic AMP.
Although little is known about the regulating mechanism of UidR, it has been shown to act as a repressor, binding to a putative inverted repeat sequence in a cooperative process with UxuR [1, 3, 4]. In 1986, Blanco et al. proposed that total repression of UidR is achieved in the presence of UxuR, suggesting the possibility that UidR and UxuR form a complex. Independently, each repressor binds to DNA separately [1, 3, 7].


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
UidR     nd nd
Evolutionary Family: TetR/AcrR
Sensing class: External sensing using transported metabolites
Connectivity class: Local Regulator
Gene name: uidR
  Genome position: 1696462-1697052
  Length: 591 bp / 196 aa
Operon name: uidR
TU(s) encoding the TF:
Transcription unit        Promoter
uidR
uidRp


Regulon       
Regulated gene(s) uidA, uidB, uidC, uidR
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
carbon compounds (3)
Porters (Uni-, Sym- and Antiporters) (1)
membrane (1)
Transcription related (1)
repressor (1)
Regulated operon(s) uidABC, uidR
First gene in the operon(s) uidA, uidR
Simple and complex regulons CRP,UidR,UxuR
UidR
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[UidR,-](1)


Transcription factor binding sites (TFBSs) arrangements       

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  UidR repressor uidAp nd -153.5 -234.5 uidA, uidB, uidC
tcgttcccaaTACGCTCGAACGAACGttcggttgct
1696298 1696313 [GEA] [1], [2], [3]
  UidR repressor uidRp Sigma70 nd nd uidR nd nd [GEA] [2]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [GEA] Gene expression analysis



Reference(s)    

 [1] Blanco C., 1987, Transcriptional and translational signals of the uidA gene in Escherichia coli K12., Mol Gen Genet. 208(3):490-8

 [2] Blanco C., Mata-Gilsinger M., Ritzenthaler P., 1985, The use of gene fusions to study the expression of uidR, a negative regulatory gene of Escherichia coli K-12., Gene. 36(1-2):159-67

 [3] Blanco C., Ritzenthaler P., Mata-Gilsinger M., 1986, Negative dominant mutations of the uidR gene in Escherichia coli: genetic proof for a cooperative regulation of uidA expression., Genetics. 112(2):173-82

 [4] Ritzenthaler P., Blanco C., Mata-Gilsinger M., 1983, Interchangeability of repressors for the control of the uxu and uid operons in E. coli K12., Mol Gen Genet. 191(2):263-70

 [5] Blanco C., Ritzenthaler P., Mata-Gilsinger M., 1982, Cloning and endonuclease restriction analysis of uidA and uidR genes in Escherichia coli K-12: determination of transcription direction for the uidA gene., J Bacteriol. 149(2):587-94

 [6] Novel M., Novel G., 1976, Regulation of beta-glucuronidase synthesis in Escherichia coli K-12: pleiotropic constitutive mutations affecting uxu and uidA expression., J Bacteriol. 127(1):418-32

 [7] Blanco C., Ritzenthaler P., Mata-Gilsinger M., 1985, Nucleotide sequence of a regulatory region of the uidA gene in Escherichia coli K12., Mol Gen Genet. 199(1):101-5



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