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SlyA DNA-binding transcriptional activator

Synonyms: SlyA
SlyA (for hemolytic protein in Salmonella ) was initially identified in Salmonella, although little is known about the regulator role of SlyA. It has been demonstrated that SlyA increases expression of hemolysin E by antagonizing the negative effects of H-NS [2, 3]
SlyA belongs to the MarR family of transcriptional regulators. This protein consist of two domains, an amino-terminal domain involved in dimerization and a carboxy-terminal domain indispensable for DNA recognition [6]
It is known that SlyA recognizes a short palindromic DNA sequence of 12 bp [3]

Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
SlyA     nd nd
Evolutionary Family: MarR
Connectivity class: Local Regulator
Gene name: slyA
  Genome position: 1720390-1720824
  Length: 435 bp / 144 aa
Operon name: slyA
TU(s) encoding the TF:
Transcription unit        Promoter

Regulated gene(s) hlyE
Multifun term(s) of regulated gene(s)
cell killing (1)
Regulated operon(s) hlyE
First gene in the operon(s) hlyE
Simple and complex regulons CRP,FNR,Fis,H-NS,SlyA
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)

Transcription factor binding sites (TFBSs) arrangements       

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  SlyA activator hlyEp nd -55.5 -127.5 hlyE
1230516 1230527 [BPP], [GEA] [1], [2], [3]
  SlyA activator hlyEp nd -44.5 -116.5 hlyE
1230505 1230516 [BPP], [GEA] [1], [2], [3], [4], [5]
  SlyA activator hlyEp nd 52.5 -20.5 hlyE
1230409 1230420 [BPP], [GEA] [1]
  SlyA activator hlyEp nd 61.5 -11.5 hlyE
1230400 1230411 [BPP], [GEA] [1]
  SlyA activator hlyEp nd 76.5 3.5 hlyE
1230385 1230396 [BPP], [GEA] [1]
  SlyA activator hlyEp nd 90.5 17.5 hlyE
1230371 1230382 [BPP], [GEA] [1]

Alignment and PSSM for SlyA TFBSs    

Position weight matrix (PWM).   
A	6	0	4	2	1	1	5	3	5	0	6
C	0	0	1	0	0	0	0	2	0	0	0
G	0	1	0	1	0	1	1	0	0	6	0
T	0	5	1	3	5	4	0	1	1	0	0

PWM logo   


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
TF-target gene evolutionary conservation
Promoter-target gene evolutionary conservation


 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis


 [1] Lithgow JK., Haider F., Roberts IS., Green J., 2007, Alternate SlyA and H-NS nucleoprotein complexes control hlyE expression in Escherichia coli K-12., Mol Microbiol. 66(3):685-98

 [2] Ludwig A., Bauer S., Benz R., Bergmann B., Goebel W., 1999, Analysis of the SlyA-controlled expression, subcellular localization and pore-forming activity of a 34 kDa haemolysin (ClyA) from Escherichia coli K-12., Mol Microbiol. 31(2):557-67

 [3] Wyborn NR., Stapleton MR., Norte VA., Roberts RE., Grafton J., Green J., 2004, Regulation of Escherichia coli hemolysin E expression by H-NS and Salmonella SlyA., J Bacteriol. 186(6):1620-8

 [4] Oscarsson J., Mizunoe Y., Uhlin BE., Haydon DJ., 1996, Induction of haemolytic activity in Escherichia coli by the slyA gene product., Mol Microbiol. 20(1):191-9

 [5] Westermark M., Oscarsson J., Mizunoe Y., Urbonaviciene J., Uhlin BE., 2000, Silencing and activation of ClyA cytotoxin expression in Escherichia coli., J Bacteriol. 182(22):6347-57

 [6] Wu RY., Zhang RG., Zagnitko O., Dementieva I., Maltzev N., Watson JD., Laskowski R., Gornicki P., Joachimiak A., 2003, Crystal structure of Enterococcus faecalis SlyA-like transcriptional factor., J Biol Chem. 278(22):20240-4