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SlyA DNA-binding transcriptional activator

Synonyms: SlyA
Summary:
SlyA (for hemolytic protein in Salmonella ) was initially identified in Salmonella, although little is known about the regulator role of SlyA. It has been demonstrated that SlyA increases expression of hemolysin E by antagonizing the negative effects of H-NS [2, 3]
SlyA belongs to the MarR family of transcriptional regulators. This protein consist of two domains, an amino-terminal domain involved in dimerization and a carboxy-terminal domain indispensable for DNA recognition [6]
It is known that SlyA recognizes a short palindromic DNA sequence of 12 bp [3]


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
SlyA     nd nd
Evolutionary Family: MarR
Connectivity class: Local Regulator
Gene name: slyA
  Genome position: 1720390-1720824
  Length: 435 bp / 144 aa
Operon name: slyA
TU(s) encoding the TF:
Transcription unit        Promoter
slyA
null


Regulon       
Regulated gene(s) hlyE
Multifun term(s) of regulated gene(s)
cell killing (1)
Regulated operon(s) hlyE
First gene in the operon(s) hlyE
Simple and complex regulons CRP,FNR,Fis,H-NS,SlyA
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[SlyA,+](1)


Transcription factor binding sites (TFBSs) arrangements       

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  SlyA activator hlyEp nd -55.5 -127.5 hlyE
tgtttgatatTTATCATATTAAtagaaataaa
1230516 1230527 [BPP], [GEA] [1], [2], [3]
  SlyA activator hlyEp nd -44.5 -116.5 hlyE
tatcatattaATAGAAATAAAGacattgacgc
1230505 1230516 [BPP], [GEA] [1], [2], [3], [4], [5]
  SlyA activator hlyEp nd 52.5 -20.5 hlyE
tcattacaatTTATATATTTAAagaggcgaat
1230409 1230420 [BPP], [GEA] [1]
  SlyA activator hlyEp nd 61.5 -11.5 hlyE
tttatatattTAAAGAGGCGAAtgattatgac
1230400 1230411 [BPP], [GEA] [1]
  SlyA activator hlyEp nd 76.5 3.5 hlyE
aggcgaatgaTTATGACTGAAAtcgttgcaga
1230385 1230396 [BPP], [GEA] [1]
  SlyA activator hlyEp nd 90.5 17.5 hlyE
gactgaaatcGTTGCAGATAAAacggtagaag
1230371 1230382 [BPP], [GEA] [1]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
TF-target gene evolutionary conservation
Promoter-target gene evolutionary conservation


Evidence    

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis



Reference(s)    

 [1] Lithgow JK., Haider F., Roberts IS., Green J., 2007, Alternate SlyA and H-NS nucleoprotein complexes control hlyE expression in Escherichia coli K-12., Mol Microbiol. 66(3):685-98

 [2] Ludwig A., Bauer S., Benz R., Bergmann B., Goebel W., 1999, Analysis of the SlyA-controlled expression, subcellular localization and pore-forming activity of a 34 kDa haemolysin (ClyA) from Escherichia coli K-12., Mol Microbiol. 31(2):557-67

 [3] Wyborn NR., Stapleton MR., Norte VA., Roberts RE., Grafton J., Green J., 2004, Regulation of Escherichia coli hemolysin E expression by H-NS and Salmonella SlyA., J Bacteriol. 186(6):1620-8

 [4] Oscarsson J., Mizunoe Y., Uhlin BE., Haydon DJ., 1996, Induction of haemolytic activity in Escherichia coli by the slyA gene product., Mol Microbiol. 20(1):191-9

 [5] Westermark M., Oscarsson J., Mizunoe Y., Urbonaviciene J., Uhlin BE., 2000, Silencing and activation of ClyA cytotoxin expression in Escherichia coli., J Bacteriol. 182(22):6347-57

 [6] Wu RY., Zhang RG., Zagnitko O., Dementieva I., Maltzev N., Watson JD., Laskowski R., Gornicki P., Joachimiak A., 2003, Crystal structure of Enterococcus faecalis SlyA-like transcriptional factor., J Biol Chem. 278(22):20240-4



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