RegulonDB RegulonDB 9.3:Regulon Page
   

NanR DNA-binding transcriptional dual regulator

Synonyms: NanR, NanR-N-acetylneuraminate
Summary:
The genes regulated by NanR, N-acetyl-neuraminic acid regulator, are involved in N-acetyl-neuraminic acid (or sialic acid) transport and metabolism [6]and in OFF/ON switching of type 1 fimbriation. N-acetylneuraminate (Neu5Ac), which is the most common sialic acid, induces the catabolism of sialic acids operons by directly inactivating NanR [1] converting the predominantly dimeric form of the repressor to an inactive monomer [4]
NanR is a member of the FadR/GntR family. Members of this family have two domains, an N-terminal domain with a helix-turn-helix DNA-binding motif and a C-terminal domain with dimerization and effector-binding motifs. Three-dimensional models of the N terminus of FadR and NanR show topological similarities and a ~26% sequence identity between them [6]
NanR regulates transcription when it binds to a region of ~30 bp that contains three conserved motifs in tandem.
Read more >


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
NanR Functional   Apo [BPP] [1]
NanR-N-acetylneuraminate Non-Functional Allosteric Holo [BPP] [1]
Evolutionary Family: GntR
Sensing class: External sensing using transported metabolites
Connectivity class: Local Regulator
Gene name: nanR
  Genome position: 3373698-3374489
  Length: 792 bp / 263 aa
Operon name: nanR
TU(s) encoding the TF:
Transcription unit        Promoter
nanR
null


Regulon       
Regulated gene(s) fimB, nanA, nanC, nanE, nanK, nanM, nanS, nanT, yhcH, yjhB, yjhC
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
amino sugar conversions (4)
carbon compounds (2)
Porters (Uni-, Sym- and Antiporters) (2)
membrane (2)
fimbri, pili (1)
Read more >
Regulated operon(s) fimB, nanATEK-yhcH, nanCMS, yjhBC
First gene in the operon(s) fimB, nanA, nanA, nanC, nanC, yjhB
Simple and complex regulons BasR,H-NS,IHF,NagC,NanR
CRP,Fis,NanR
CRP,NagC,NanR
NanR
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[NanR,+](1)
[NanR,-](3)


Transcription factor binding sites (TFBSs) arrangements       

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence
LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  NanR activator fimBp2 nd -744.5 -1034.5 fimB
actatcttggTGCACCtttatacctg
4539920 4539925 [BPP], [GEA], [HIBSCS], [SM] [1], [2], [3], [4], [5]
  NanR activator fimBp2 nd -736.5 -1026.5 fimB
ggtgcaccttTATACCtgttatacca
4539928 4539933 [BPP], [GEA], [HIBSCS], [SM] [1], [2], [3], [4], [5]
  NanR activator fimBp2 nd -727.5 -1017.5 fimB
ttatacctgtTATACCagatcaaaaa
4539937 4539942 [BPP], [GEA], [HIBSCS], [SM] [1], [2], [3], [4], [5]
  NanR repressor nanAp Sigma70 -11.5 -55.5 nanA, nanT, nanE, nanK, yhcH
taattgatctGGTATAacaggtataa
3373629 3373634 [BPP], [GEA], [SM] [4], [6], [7]
  NanR repressor nanAp Sigma70 -2.5 -46.5 nanA, nanT, nanE, nanK, yhcH
tggtataacaGGTATAaaggtatatc
3373620 3373625 [BPP], [GEA], [SM] [4], [6], [7]
  NanR repressor nanAp Sigma70 6.5 -38.5 nanA, nanT, nanE, nanK, yhcH
caggtataaaGGTATAtcgtttatca
3373612 3373617 [BPP], [GEA], [SM] [4], [6], [7]
  NanR repressor nanCp nd -11.5 -438.5 nanC, nanM, nanS
tttttgatctGGTATAacaggtataa
4539937 4539942 [BPP], [GEA], [HIBSCS], [SM] [1], [2], [3], [4], [5]
  NanR repressor nanCp nd -2.5 -429.5 nanC, nanM, nanS
tggtataacaGGTATAaaggtgcacc
4539928 4539933 [BPP], [GEA], [HIBSCS], [SM] [1], [2], [3], [4], [5]
  NanR repressor nanCp nd 6.5 -421.5 nanC, nanM, nanS
caggtataaaGGTGCAccaagatagt
4539920 4539925 [BPP], [GEA], [HIBSCS], [SM] [1], [2], [3], [4], [5]
  NanR repressor yjhBp2 Sigma32 -393.5 -431.5 yjhB, yjhC
tttttgatctGGTATAacaggtataa
4503624 4503629 [BPP], [GEA], [SM] [4]
  NanR repressor yjhBp2 Sigma32 -384.5 -422.5 yjhB, yjhC
tggtataacaGGTATAaaggtataca
4503633 4503638 [BPP], [GEA], [SM] [4]
  NanR repressor yjhBp2 Sigma32 -376.5 -414.5 yjhB, yjhC
caggtataaaGGTATAcagaaaagca
4503641 4503646 [BPP], [GEA], [SM] [4]


Alignment and PSSM for NanR TFBSs    

Aligned TFBS of NanR   
  Sequence
 

Position weight matrix (PWM).   
A	6	0	0	0	8	0	9
C	0	0	0	0	0	1	0
G	0	9	9	0	1	0	0
T	3	0	0	9	0	8	0

PWM logo   


 


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
TF-target gene evolutionary conservation
Promoter-target gene evolutionary conservation


Evidence    

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [HIBSCS] Human inference based on similarity to consensus sequences

 [SM] Site mutation



Reference(s)    

 [1] Sohanpal BK., El-Labany S., Lahooti M., Plumbridge JA., Blomfield IC., 2004, Integrated regulatory responses of fimB to N-acetylneuraminic (sialic) acid and GlcNAc in Escherichia coli K-12., Proc Natl Acad Sci U S A. 101(46):16322-7

 [2] Condemine G., Berrier C., Plumbridge J., Ghazi A., 2005, Function and expression of an N-acetylneuraminic acid-inducible outer membrane channel in Escherichia coli., J Bacteriol. 187(6):1959-65

 [3] El-Labany S., Sohanpal BK., Lahooti M., Akerman R., Blomfield IC., 2003, Distant cis-active sequences and sialic acid control the expression of fimB in Escherichia coli K-12., Mol Microbiol. 49(4):1109-18

 [4] Kalivoda KA., Steenbergen SM., Vimr ER., 2013, Control of the Escherichia coli sialoregulon by transcriptional repressor NanR., J Bacteriol. 195(20):4689-701

 [5] Sohanpal BK., Friar S., Roobol J., Plumbridge JA., Blomfield IC., 2007, Multiple co-regulatory elements and IHF are necessary for the control of fimB expression in response to sialic acid and N-acetylglucosamine in Escherichia coli K-12., Mol Microbiol. 63(4):1223-36

 [6] Kalivoda KA., Steenbergen SM., Vimr ER., Plumbridge J., 2003, Regulation of sialic acid catabolism by the DNA binding protein NanR in Escherichia coli., J Bacteriol. 185(16):4806-15

 [7] Plumbridge J., Vimr E., 1999, Convergent pathways for utilization of the amino sugars N-acetylglucosamine, N-acetylmannosamine, and N-acetylneuraminic acid by Escherichia coli., J Bacteriol. 181(1):47-54

 [8] Chu D., Roobol J., Blomfield IC., 2008, A theoretical interpretation of the transient sialic acid toxicity of a nanR mutant of Escherichia coli., J Mol Biol. 375(3):875-89

 [9] Oshima T., Wada C., Kawagoe Y., Ara T., Maeda M., Masuda Y., Hiraga S., Mori H., 2002, Genome-wide analysis of deoxyadenosine methyltransferase-mediated control of gene expression in Escherichia coli., Mol Microbiol. 45(3):673-95



RegulonDB