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Synonyms: NanR-N-acetylneuraminate, NanR |
Summary:
The genes regulated by NanR, "N-acetyl-neuraminic acid regulator," are involved in N-acetyl-neuraminic acid (or sialic acid) transport and metabolism [7] and in OFF/ON switching of type 1 fimbriation. N-acetylneuraminate (Neu5Ac), which is the most common sialic acid, induces the catabolism of sialic acids operons by directly inactivating NanR [1], converting the predominantly dimeric form of the repressor to an inactive monomer [4]. NanR is a member of the FadR/GntR family. Members of this family have two domains, an N-terminal domain with a helix-turn-helix DNA-binding motif and a C-terminal domain with dimerization and effector-binding motifs. Three-dimensional models of the N terminus of FadR and NanR show topological similarities and a ~26% sequence identity between them [7]. The NanR dimer regulates transcription when it binds, in an asymmetric manner, to a region of ~30 bp that contains a set of conserved operators with two or three exact, or nearly exact, repeats of the hexanucleotide sequence GGTATA; this binding occurs in a cooperative way and is mediated by an N-terminal extension of 32 residues in NanR [4, 9] However, this protein can be displaced from this region byN-acetyl-neuraminic acid (Neu5Ac), which causes a conformational change in the protein [9] One dimer of NarR binding to a molecule of Neu5Ac causes alteration of the DNA binding activity of the protein but not its oligomeric state [9] When this protein is repressing transcription, it overlaps the whole promoter region [1, 7]. Read more > |
Transcription factor | ![]() ![]() |
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TF conformation(s): |
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Evolutionary Family: | GntR | ||||||||||||||||||
Sensing class: | External sensing using transported metabolites | ||||||||||||||||||
Connectivity class: | Local Regulator | ||||||||||||||||||
Gene name: | nanR | ||||||||||||||||||
Genome position: | 3373698-3374489 | ||||||||||||||||||
Length: | 792 bp / 263 aa | ||||||||||||||||||
Operon name: | nanR | ||||||||||||||||||
TU(s) encoding the TF: |
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Regulon |
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Regulated gene(s) | fimB, nanA, nanC, nanE, nanK, nanM, nanQ, nanS, nanT, nanX, nanY | ||||||
Multifun term(s) of regulated gene(s) |
MultiFun Term (List of genes associated to the multifun term)
amino sugar conversions (4)
carbon compounds (3)
Porters (Uni-, Sym- and Antiporters) (2)
membrane (2)
fimbri, pili (1)
Read more >
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Regulated operon(s) | fimB, nanATEKQ, nanCMS, nanXY | ||||||
First gene in the operon(s) | fimB, nanA, nanC, nanX | ||||||
Simple and complex regulons | BasR,DksA,DksA-ppGpp,H-NS,IHF,NagC,NanR,ppGpp CRP,Fis,NanR CRP,NagC,NanR NanR | ||||||
Simple and complex regulatory phrases | Regulatory phrase (List of promoters regulated by the phrase) |
Transcription factor regulation |
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Functional conformation | Function | Promoter | Sigma factor | Central Rel-Pos | Distance to first Gene | Genes | Sequence | LeftPos | RightPos | Evidence (Confirmed, Strong, Weak) | References | |
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NanR | activator | fimBp2 | nd | -744.5 | -1034.5 | fimB | 4539922 | 4539923 | [GEA], [APIORCISFBSCS], [BPP], [GS], [SM] | [1], [2], [3], [4], [5], [6] | ||
NanR | activator | fimBp2 | nd | -736.5 | -1026.5 | fimB | 4539930 | 4539931 | [GEA], [APIORCISFBSCS], [BPP], [GS], [SM] | [1], [2], [3], [4], [5], [6] | ||
NanR | activator | fimBp2 | nd | -727.5 | -1017.5 | fimB | 4539939 | 4539940 | [GEA], [APIORCISFBSCS], [BPP], [GS], [SM] | [1], [2], [3], [4], [5], [6] | ||
NanR | repressor | nanAp | Sigma70 | -11.5 | -55.5 | nanA, nanT, nanE, nanK, nanQ | 3373631 | 3373632 | [GEA], [BPP], [SM] | [4], [7], [8] | ||
NanR | repressor | nanAp | Sigma70 | -2.5 | -46.5 | nanA, nanT, nanE, nanK, nanQ | 3373622 | 3373623 | [GEA], [BPP], [SM] | [4], [7], [8] | ||
NanR | repressor | nanAp | Sigma70 | 6.5 | -38.5 | nanA, nanT, nanE, nanK, nanQ | 3373614 | 3373615 | [GEA], [BPP], [SM] | [4], [7], [8] | ||
NanR | repressor | nanCp | nd | -11.5 | -438.5 | nanC, nanM, nanS | 4539939 | 4539940 | [GEA], [APIORCISFBSCS], [BPP], [GS], [SM] | [2], [3], [4], [5] | ||
NanR | repressor | nanCp | nd | -2.5 | -429.5 | nanC, nanM, nanS | 4539930 | 4539931 | [GEA], [APIORCISFBSCS], [BPP], [GS], [SM] | [2], [3], [4], [5] | ||
NanR | repressor | nanCp | nd | 6.5 | -421.5 | nanC, nanM, nanS | 4539922 | 4539923 | [GEA], [APIORCISFBSCS], [BPP], [GS], [SM] | [2], [3], [4], [5] | ||
NanR | repressor | yjhBp2 | Sigma32 | -393.5 | -431.5 | nanX, nanY | 4503626 | 4503627 | [GEA], [BPP], [SM] | [4] | ||
NanR | repressor | yjhBp2 | Sigma32 | -384.5 | -422.5 | nanX, nanY | 4503635 | 4503636 | [GEA], [BPP], [SM] | [4] | ||
NanR | repressor | yjhBp2 | Sigma32 | -376.5 | -414.5 | nanX, nanY | 4503643 | 4503644 | [GEA], [BPP], [SM] | [4] |
Alignment and PSSM for NanR TFBSs | ![]() |
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Aligned TFBS of NanR |
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Sequence | |
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TATACCT | |
TATACCT | |
TATACCT | |
TATACCT | |
TATACCT | |
TATACCA | |
TATACCA | |
TATACCA | |
TGCACCT |
Position weight matrix (PWM). NanR matrix-quality result |
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A 0 8 0 9 0 0 3 C 0 0 1 0 9 9 0 G 0 1 0 0 0 0 0 T 9 0 8 0 0 0 6 |
Consensus |
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; consensus.strict TATACCt ; consensus.strict.rc AGGTATA ; consensus.IUPAC TATACCw ; consensus.IUPAC.rc WGGTATA ; consensus.regexp TATACC[at] ; consensus.regexp.rc [AT]GGTATA |
Evolutionary conservation of regulatory elements | ![]() |
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Reference(s) |
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