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Synonyms: PurR, PurR-Guanine, PurR-hypoxanthine |
Summary:
PurR dimer controls several genes involved in purine nucleotide biosynthesis and its own synthesis [11, 12, 22]. This regulator necessarily binds two products of purine metabolism, hypoxanthine and guanine, to induce the conformational change that allows PurR to bind DNA []. PurR belongs to the GalR/LacI family [2], and it shows 35%, 33%, and 26% identity with CytR, GalR, and LacI, respectively [5]. As with other members of this group, PurR shows a helix-turn-helix motif in the N-terminal domain for DNA binding [5] and a ligand-binding and dimerization motif in the C-terminal domain that shows similarity with ligand binding sites of periplasmic sugar-binding proteins [][2] The two domains are connected by a hinge sequence that is also important for DNA binding and that can be cleavage by proteases when the protein is not bound to DNA []. Read more > |
Transcription factor | ![]() ![]() |
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TF conformation(s): |
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Evolutionary Family: | GalR/LacI | ||||||||||||||||||||||||
Sensing class: | Using internal synthesized signals | ||||||||||||||||||||||||
Connectivity class: | Local Regulator | ||||||||||||||||||||||||
Gene name: | purR | ||||||||||||||||||||||||
Genome position: | 1737844-1738869 | ||||||||||||||||||||||||
Length: | 1026 bp / 341 aa | ||||||||||||||||||||||||
Operon name: | purR | ||||||||||||||||||||||||
TU(s) encoding the TF: |
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Regulon |
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Regulated gene(s) | carA, carB, codA, codB, cvpA, gcvH, gcvP, gcvT, glnB, glyA, guaA, guaB, hflD, prs, purA, purB, purC, purD, purE, purF, purH, purK, purL, purM, purN, purR, purT, pyrC, pyrD, speA, speB, ubiX | ||||||
Multifun term(s) of regulated gene(s) |
MultiFun Term (List of genes associated to the multifun term)
purine biosynthesis (15)
nucleotide and nucleoside conversions (7)
pyrimidine biosynthesis (5)
amino acids (5)
formyl-THF biosynthesis (5)
Read more >
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Regulated operon(s) | carAB, codBA, cvpA-purF-ubiX, gcvTHP, glrR-glnB, glyA, guaBA, hflD-purB, lolB-ispE-prs, purA, purC, purEK, purHD, purL, purMN, purR, purT, pyrC, pyrD, speAB | ||||||
First gene in the operon(s) | carA, codB, cvpA, gcvT, glnB, glnB, glyA, guaB, hflD, prs, purA, purC, purE, purH, purL, purM, purR, purT, pyrC, pyrD, speA | ||||||
Simple and complex regulons | CRP,DnaA,Fis,PurR CRP,FNR,GcvA,Lrp,PurR FNR,PurR Fis,IHF,PepA,PurR,RutR Fis,PurR Read more > | ||||||
Simple and complex regulatory phrases | Regulatory phrase (List of promoters regulated by the phrase) |
Transcription factor regulation |
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Functional conformation | Function | Promoter | Sigma factor | Central Rel-Pos | Distance to first Gene | Genes | Sequence | LeftPos | RightPos | Evidence (Confirmed, Strong, Weak) | References | |
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PurR-hypoxanthine | repressor | carAp1 | Sigma70 | -128.5 | -228.5 | carA, carB |
tcttcttgctGCGCAAGCGTTTTCCAgaacaggtta
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29415 | 29430 | [AIBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] | [6], [7], [8] | |
PurR-hypoxanthine | repressor | codBp | Sigma70 | -36.5 | -74.5 | codB, codA |
atatttccccACGAAAACGATTGCTTtttatcttca
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354840 | 354855 | [AIBSCS], [APIORCISFBSCS], [CV(GEA)], [GEA] | [6], [9], [10] | |
PurR-hypoxanthine | repressor | cvpAp1 | Sigma70 | -28.5 | -65.5 | cvpA, purF, ubiX |
aggaaatcccTACGCAAACGTTTTCTttttctgtta
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2430821 | 2430836 | [APIORCISFBSCS], [BCE], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] | [5], [6], [11], [12], [13] | |
PurR-hypoxanthine | repressor | gcvTp | Sigma70 | 7.5 | -98.5 | gcvT, gcvH, gcvP |
gttcaggcaaAAGAGAACGATTGCGTtggggaccgg
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3050758 | 3050773 | [AIBSCS], [APIORCISFBSCS], [CV(GEA)], [GEA] | [6], [14] | |
PurR-hypoxanthine | repressor | glnBp1 | Sigma70 | -42.5 | -75.5 | glnB |
cacgagctggATGCAAACGATTTCAAggaatgaatt
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2687476 | 2687491 | [APIORCISFBSCS], [BCE], [CV(GEA)], [GEA] | [15] | |
PurR-hypoxanthine | repressor | glnBp2 | Sigma70 | 20.5 | -75.5 | glnB |
cacgagctggATGCAAACGATTTCAAggaatgaatt
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2687476 | 2687491 | [APIORCISFBSCS], [BCE], [CV(GEA)], [GEA] | [15] | |
PurR-hypoxanthine | repressor | glyAp | Sigma70 | -59.5 | -126.5 | glyA |
cgtagcctgaAGGTAATCGTTTGCGTaaattccttt
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2685626 | 2685641 | [AIBSCS], [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] | [6], [16], [17], [18] | |
PurR-hypoxanthine | repressor | guaBp | Sigma70 | -24.5 | -61.5 | guaB, guaA |
aaaggggtagATGCAATCGGTTACGCtctgtataat
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2634124 | 2634139 | [AIBSCS], [BCE], [CV(GEA)], [GEA] | [6], [12] | |
PurR-hypoxanthine | repressor | hflDp | Sigma70 | 892.5 | 837.5 | hflD, purB |
tttgctgccgACGCAATCGGTTACCTtgatgcaatc
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1191787 | 1191802 | [AIBSCS], [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] | [3], [6] | |
PurR-hypoxanthine | repressor | prsp | Sigma70 | -48.5 | -350.5 | prs |
aggttatcgcAAGAAAACGTTTTCGCgaggttgatg
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1262218 | 1262233 | [APIORCISFBSCS], [BCE], [CV(GEA)], [GEA] | [15] | |
PurR-hypoxanthine | repressor | purAp | Sigma70 | -90.5 | -113.5 | purA |
ctacatgttgAGGAAAACGATTGGCTgaacaaaaaa
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4404566 | 4404581 | [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] | [4], [6] | |
PurR-hypoxanthine | repressor | purAp | Sigma70 | 9.5 | -14.5 | purA |
atccatttttAAGCAAACGGTGATTTtgaaaaatgg
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4404665 | 4404680 | [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] | [4], [6] | |
PurR-hypoxanthine | repressor | purCp | Sigma70 | 2.5 | -161.5 | purC |
ctgatatgatACGCAAACGTGTGCGTctgcaggaaa
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2597772 | 2597787 | [AIBSCS], [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] | [6], [11] | |
PurR-hypoxanthine | repressor | purEp | Sigma70 | -37.5 | -79.5 | purE, purK |
tttcacagccACGCAACCGTTTTCCTtgctctcttt
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553172 | 553187 | [AIBSCS], [APIORCISFBSCS], [BCE], [CV(GEA)], [GEA] | [6], [11], [12], [19] | |
PurR-hypoxanthine | repressor | purHp | Sigma70 | -20.5 | -115.5 | purH, purD |
cgcgagcgttGCGCAAACGTTTTCGTtacaatgcgg
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4207640 | 4207655 | [AIBSCS], [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] | [6], [11] | |
PurR-hypoxanthine | repressor | purLp | Sigma70 | -38.5 | -84.5 | purL |
ttttatttccACGCAAACGGTTTCGTcagcgcatca
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2695620 | 2695635 | [AIBSCS], [APIORCISFBSCS], [BCE], [CV(GEA)], [GEA] | [6], [11], [12] | |
PurR-hypoxanthine | repressor | purMp | Sigma70 | -27.5 | -71.5 | purM, purN |
taaagcagtcTCGCAAACGTTTGCTTTccctgttaga
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2621118 | 2621134 | [AIBSCS], [APIORCISFBSCS], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] | [6], [12], [20], [21] | |
PurR-hypoxanthine | repressor | purRp | Sigma70 | 103.5 | -52.5 | purR |
aggtgtgtaaAGGCAAACGTTTACCTtgcgattttg
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1737784 | 1737799 | [APIORCISFBSCS], [BCE], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] | [6], [12], [22] | |
PurR-hypoxanthine | repressor | purRp | Sigma70 | 191.5 | 36.5 | purR |
gtagcgaaacGAGCAAACGTTTCCACtacaactgtg
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1737872 | 1737887 | [APIORCISFBSCS], [BCE], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] | [12], [22] | |
PurR-hypoxanthine | repressor | purTp | Sigma70 | -21.5 | -45.5 | purT |
ataaagacacACGCAAACGTTTTCGTTtatactgcgc
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1930828 | 1930844 | [AIBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] | [23] | |
PurR-hypoxanthine | repressor | pyrCp | Sigma70 | -23.5 | -59.5 | pyrC |
tttcgtgcaaAGGAAAACGTTTCCGCttatcctttg
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1122659 | 1122674 | [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] | [1], [24], [25] | |
PurR-hypoxanthine | repressor | pyrDp | Sigma70 | -58.5 | -94.5 | pyrD |
gaaacaggttCGGAAAACGTTTGCGTtttttttgcc
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1004666 | 1004681 | [AIBSCS], [APIORCISFBSCS], [BCE], [CV(GEA)], [GEA] | [6], [25] | |
PurR-hypoxanthine | repressor | speAp | Sigma70 | 11.5 | -126.5 | speA, speB |
tgttatgaaaAAGAAACCGGTTGCGCagttggagcg
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3086030 | 3086045 | [APIORCISFBSCS], [BCE], [CV(GEA)], [GEA] | [15] |
Alignment and PSSM for PurR TFBSs | ![]() |
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Aligned TFBS of PurR |
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Sequence | |
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ACGCAAACGTTTTCGTT | |
ACGCAAACGTTTTCTTT | |
GCGCAAACGTTTTCGTT | |
ACGCAACCGTTTTCCTT | |
ACGCAAACGGTTTCGTC | |
ACGCAATCGGTTACCTT | |
ACGCAAACGATTACCTT | |
AGGCAAACGTTTACCTT | |
GCGCAACCGGTTTCTTT | |
GCGGAAACGTTTTCCTT | |
AAGCAATCGTTTTCGTG | |
ACGCAATCGTTCTCTTT | |
TCGCAAACGTTTGCTTT | |
ACGCAAACGTTTTCCGA | |
GCGAAAACGTTTTCTTG | |
ACGCAAACGTGTGCGTC | |
GCGCAAGCGTTTTCCAG | |
AGCCAATCGTTTTCCTC | |
ATGCAATCGGTTACGCT | |
GAGCAAACGTTTCCACT | |
ATGCAAACGATTTCAAG | |
AAGCAAACGGTGATTTT |
Position weight matrix (PWM). PurR matrix-quality result |
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A 15 3 0 1 22 22 14 0 0 2 0 0 5 0 2 2 1 C 0 15 1 20 0 0 2 22 0 0 0 1 1 21 8 2 3 G 6 2 21 1 0 0 1 0 22 5 1 1 2 0 6 1 4 T 1 2 0 0 0 0 5 0 0 15 21 20 14 1 6 17 14 |
Consensus |
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; consensus.strict aCGCAAaCGtTTtCctt ; consensus.strict.rc AAGGAAAACGTTTGCGT ; consensus.IUPAC rCGCAAaCGkTTtCstt ; consensus.IUPAC.rc AASGAAAMCGTTTGCGY ; consensus.regexp [ag]CGCAAaCG[gt]TTtC[cg]tt ; consensus.regexp.rc AA[CG]GAAA[AC]CGTTTGCG[CT] |
Evolutionary conservation of regulatory elements | ![]() |
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Reference(s) |
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