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IlvY DNA-binding transcriptional dual regulator

Synonyms: IlvY-Alfa-acetohydroxybutirate, IlvY-2-acetolactate, IlvY


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
IlvY Functional   Apo [BPP], [IDA], [IPI], [SM] [1]
IlvY-2-acetolactate Functional Allosteric Holo [BPP], [IDA], [IPI], [SM] [1]
IlvY-Alfa-acetohydroxybutirate Functional Allosteric Holo [BPP], [IDA], [IPI], [SM] [1]
Evolutionary Family: LysR
Sensing class: Using internal synthesized signals
Connectivity class: Local Regulator
Gene name: ilvY
  Genome position: 3956927-3957820
  Length: 894 bp / 297 aa
Operon name: ilvY
TU(s) encoding the TF:
Transcription unit        Promoter
ilvY
ilvYp


Regulon       
Regulated gene(s) ilvC, ilvY
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
isoleucine/valine (2)
Transcription related (1)
activator (1)
repressor (1)
operon (1)
Regulated operon(s) ilvC, ilvY
First gene in the operon(s) ilvC, ilvY
Simple and complex regulons IlvY
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[IlvY,-](1)
[IlvY,+](1)


Transcription factor binding sites (TFBSs) arrangements       

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  IlvY-2-acetolactate activator ilvCp Sigma70 -61.0 -119.0 ilvC
cgatgttgacGTTGCAAAAATTGCAATgtgacgttgt
3957843 3957859 [BCE], [BPP], [GEA], [HIBSCS], [IC] [2], [3], [4], [5], [6]
  IlvY activator ilvCp Sigma70 -30.0 -88.0 ilvC
cgttgtgaatATATCAATTTCCGCAATaaatttcctg
3957874 3957890 [BCE], [BPP], [GEA], [HIBSCS], [IC] [4], [6]
  IlvY repressor ilvYp Sigma70 -15.0 -62.0 ilvY
caggaaatttATTGCGGAAATTGATATattcacaacg
3957874 3957890 [BCE], [BPP], [GEA], [HIBSCS], [IC] [4], [6]
  IlvY-2-acetolactate repressor ilvYp Sigma70 17.0 -31.0 ilvY
acaacgtcacATTGCAATTTTTGCAACgtcaacatcg
3957843 3957859 [BCE], [BPP], [GEA], [HIBSCS], [IC] [2], [3], [4], [5], [6]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [BPP] Binding of purified proteins

 [IDA] Inferred from direct assay

 [IPI] Inferred from physical interaction

 [SM] Site mutation

 [BCE] Binding of cellular extracts

 [GEA] Gene expression analysis

 [HIBSCS] Human inference based on similarity to consensus sequences

 [IC] Inferred by curator



Reference(s)    

 [1] 9556617., null, null, null

 [2] Biel AJ., Umbarger HE., 1981, Mutations in the ilvY gene of Escherichia coli K-12 that cause constitutive expression of ilvC., J Bacteriol. 146(2):718-24

 [3] Pledger WJ., Umbarger HE., 1973, Isoleucine and valine metabolism in Escherichia coli. XXII. A pleiotropic mutation affecting induction of isomeroreductase activity., J Bacteriol. 114(1):195-207

 [4] Rhee KY., Senear DF., Hatfield GW., 1998, Activation of gene expression by a ligand-induced conformational change of a protein-DNA complex., J Biol Chem. 273(18):11257-66

 [5] Watson MD., Wild J., Umbarger HE., 1979, Positive control of ilvC expression in Escherichia coli K-12; identification and mapping of regulatory gene ilvY., J Bacteriol. 139(3):1014-20

 [6] Wek RC., Hatfield GW., 1988, Transcriptional activation at adjacent operators in the divergent-overlapping ilvY and ilvC promoters of Escherichia coli., J Mol Biol. 203(3):643-63



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