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CspA DNA-binding transcriptional activator

Synonyms: CspA
Summary:
The Cold shock protein A, CspA, is a major cold shock protein and was shown to be detected only during early-log-phase growth at 37°C and during log phase after a shift from 37°C to 10°C [5, 6]. However, studies have shown that although the expression of cspA is reduced during stationary phase, cspA mRNA and CspA are detectable during all growth phases [7]. CspA acts as a positive transcription factor of at least two cold shock genes: hns [2, 3, 4] and gyrA [1]. cspA has been shown to negatively regulate its own expression as the result of attenuation of transcription [8]. A model of how CspA might affect the transcription of hns has been proposed [9].
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Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
CspA     nd nd
Evolutionary Family: Cold
Connectivity class: Local Regulator
Gene name: cspA
  Genome position: 3720049-3720261
  Length: 213 bp / 70 aa
Operon name: cspA
TU(s) encoding the TF:
Transcription unit        Promoter
cspA
cspAp1
cspA
cspAp2


Regulon       
Regulated gene(s) gyrA, hns
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
Transcription related (2)
DNA replication (1)
nucleoproteins, basic proteins (1)
activator (1)
repressor (1)
Regulated operon(s) gyrA, hns
First gene in the operon(s) gyrA, hns
Simple and complex regulons CRP,CspA,Fis
CspA,Fis,GadX,H-NS
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[CspA,+](1)


Transcription factor binding sites (TFBSs) arrangements       

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence
LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  CspA activator gyrAp Sigma70 -108.0 -145.0 gyrA
ttcggcattcATTGGCActtctactcc
2339562 2339568 [HIBSCS] [1]
  CspA activator gyrAp Sigma70 -88.0 -125.0 gyrA
ctactccgtaATTGGCAagacaaacga
2339542 2339548 [HIBSCS] [1]
  CspA activator gyrAp Sigma70 -60.0 -97.0 gyrA
tatatcaggcATTGGATgtgaataaag
2339514 2339520 [HIBSCS] [1]
  CspA activator hnsp Sigma70 nd nd hns nd nd [BPP] [2], [3], [4]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [HIBSCS] Human inference based on similarity to consensus sequences

 [BPP] Binding of purified proteins



Reference(s)    

 [1] Jones PG., Krah R., Tafuri SR., Wolffe AP., 1992, DNA gyrase, CS7.4, and the cold shock response in Escherichia coli., J Bacteriol. 174(18):5798-802

 [2] Brandi A., Pon CL., Gualerzi CO., 1994, Interaction of the main cold shock protein CS7.4 (CspA) of Escherichia coli with the promoter region of hns., Biochimie. 76(10-11):1090-8

 [3] Giangrossi M., Gualerzi CO., Pon CL., 2001, Mutagenesis of the downstream region of the Escherichia coli hns promoter., Biochimie. 83(2):251-9

 [4] La Teana A., Brandi A., Falconi M., Spurio R., Pon CL., Gualerzi CO., 1991, Identification of a cold shock transcriptional enhancer of the Escherichia coli gene encoding nucleoid protein H-NS., Proc Natl Acad Sci U S A. 88(23):10907-11

 [5] Jones PG., VanBogelen RA., Neidhardt FC., 1987, Induction of proteins in response to low temperature in Escherichia coli., J Bacteriol. 169(5):2092-5

 [6] Goldstein J., Pollitt NS., Inouye M., 1990, Major cold shock protein of Escherichia coli., Proc Natl Acad Sci U S A. 87(1):283-7

 [7] Brandi A., Pon CL., 2012, Expression of Escherichia coli cspA during early exponential growth at 37¿¿C., Gene. 492(2):382-8

 [8] Bae W., Jones PG., Inouye M., 1997, CspA, the major cold shock protein of Escherichia coli, negatively regulates its own gene expression., J Bacteriol. 179(22):7081-8

 [9] Johnston D., Tavano C., Wickner S., Trun N., 2006, Specificity of DNA binding and dimerization by CspE from E. coli., J Biol Chem. 281(52):40208-15

 [10] Bae W., Phadtare S., Severinov K., Inouye M., 1999, Characterization of Escherichia coli cspE, whose product negatively regulates transcription of cspA, the gene for the major cold shock protein., Mol Microbiol. 31(5):1429-41

 [11] Zere TR., Vakulskas CA., Leng Y., Pannuri A., Potts AH., Dias R., Tang D., Kolaczkowski B., Georgellis D., Ahmer BM., Romeo T., 2015, Genomic Targets and Features of BarA-UvrY (-SirA) Signal Transduction Systems., PLoS One. 10(12):e0145035

 [12] Yamanaka K., Fang L., Inouye M., 1998, The CspA family in Escherichia coli: multiple gene duplication for stress adaptation., Mol Microbiol. 27(2):247-55

 [13] Didier DK., Schiffenbauer J., Woulfe SL., Zacheis M., Schwartz BD., 1988, Characterization of the cDNA encoding a protein binding to the major histocompatibility complex class II Y box., Proc Natl Acad Sci U S A. 85(19):7322-6

 [14] Schindelin H., Jiang W., Inouye M., Heinemann U., 1994, Crystal structure of CspA, the major cold shock protein of Escherichia coli., Proc Natl Acad Sci U S A. 91(11):5119-23

 [15] Newkirk K., Feng W., Jiang W., Tejero R., Emerson SD., Inouye M., Montelione GT., 1994, Solution NMR structure of the major cold shock protein (CspA) from Escherichia coli: identification of a binding epitope for DNA., Proc Natl Acad Sci U S A. 91(11):5114-8

 [16] Feng W., Tejero R., Zimmerman DE., Inouye M., Montelione GT., 1998, Solution NMR structure and backbone dynamics of the major cold-shock protein (CspA) from Escherichia coli: evidence for conformational dynamics in the single-stranded RNA-binding site., Biochemistry. 37(31):10881-96

 [17] Jiang W., Hou Y., Inouye M., 1997, CspA, the major cold-shock protein of Escherichia coli, is an RNA chaperone., J Biol Chem. 272(1):196-202

 [18] Brandi A., Giangrossi M., Giuliodori AM., Falconi M., 2016, An Interplay among FIS, H-NS, and Guanosine Tetraphosphate Modulates Transcription of the Escherichia coli cspA Gene under Physiological Growth Conditions., Front Mol Biosci. 3:19

 [19] Tanabe H., Goldstein J., Yang M., Inouye M., 1992, Identification of the promoter region of the Escherichia coli major cold shock gene, cspA., J Bacteriol. 174(12):3867-73

 [20] Brandi A., Spurio R., Gualerzi CO., Pon CL., 1999, Massive presence of the Escherichia coli 'major cold-shock protein' CspA under non-stress conditions., EMBO J. 18(6):1653-9

 [21] Hankins JS., Zappavigna C., Prud'homme-Genereux A., Mackie GA., 2007, Role of RNA structure and susceptibility to RNase E in regulation of a cold shock mRNA, cspA mRNA., J Bacteriol. 189(12):4353-8

 [22] Ivancic T., Jamnik P., Stopar D., 2013, Cold shock CspA and CspB protein production during periodic temperature cycling in Escherichia coli., BMC Res Notes. 6:248

 [23] Jiang W., Fang L., Inouye M., 1996, The role of the 5'-end untranslated region of the mRNA for CspA, the major cold-shock protein of Escherichia coli, in cold-shock adaptation., J Bacteriol. 178(16):4919-25

 [24] Jiang W., Jones P., Inouye M., 1993, Chloramphenicol induces the transcription of the major cold shock gene of Escherichia coli, cspA., J Bacteriol. 175(18):5824-8

 [25] Etchegaray JP., Inouye M., 1999, CspA, CspB, and CspG, major cold shock proteins of Escherichia coli, are induced at low temperature under conditions that completely block protein synthesis., J Bacteriol. 181(6):1827-30

 [26] Constantinidou C., Hobman JL., Griffiths L., Patel MD., Penn CW., Cole JA., Overton TW., 2006, A reassessment of the FNR regulon and transcriptomic analysis of the effects of nitrate, nitrite, NarXL, and NarQP as Escherichia coli K12 adapts from aerobic to anaerobic growth., J Biol Chem. 281(8):4802-15



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