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CspA DNA-binding transcriptional activator

Synonyms: CspA
Summary:
The "Cold shock protein A," CspA, is a major cold shock protein and was shown to be detected only during early-log-phase growth at 37°C and during log phase after a shift from 37°C to 10°C [3, 8]. However, studies have shown that although the expression of cspA is reduced during stationary phase, cspA mRNA and CspA are detectable during all growth phases []. CspA acts as a positive transcription factor of at least two cold shock genes: hns [1, 4, 7] and gyrA [6]. cspA has been shown to negatively regulate its own expression as the result of attenuation of transcription []. A model of how CspA might affect the transcription of hns has been proposed [9].
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Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
CspA Functional   [APPH], [IEP], [TASES] [1], [2], [3], [4], [5]
Evolutionary Family: Cold
Connectivity class: Local Regulator
Gene name: cspA
  Genome position: 3720049-3720261
  Length: 213 bp / 70 aa
Operon name: cspA
TU(s) encoding the TF:
Transcription unit        Promoter
cspA
cspAp1
cspA
cspAp2


Regulon       
Regulated gene(s) gyrA, hns
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
Transcription related (2)
DNA replication (1)
nucleoproteins, basic proteins (1)
activator (1)
repressor (1)
Regulated operon(s) gyrA, hns
First gene in the operon(s) gyrA, hns
Simple and complex regulons CRP,CspA,Fis
CspA,Fis,GadX,H-NS
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[CspA,+](1)


Transcription factor regulation    


Transcription factor binding sites (TFBSs) arrangements
      

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence
LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  CspA activator gyrAp Sigma70 -108.0 -145.0 gyrA
ggagtagaagTGCCAATgaatgccgaa
2339562 2339568 [HIBSCS] [6]
  CspA activator gyrAp Sigma70 -88.0 -125.0 gyrA
tcgtttgtctTGCCAATtacggagtag
2339542 2339548 [HIBSCS] [6]
  CspA activator gyrAp Sigma70 -60.0 -97.0 gyrA
ctttattcacATCCAATgcctgatata
2339514 2339520 [HIBSCS] [6]
  CspA activator hnsp Sigma70 nd 1292922.0 hns nd nd [BPP] [1], [4], [7]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [APPH] Assay of protein purified to homogeneity

 [IEP] Inferred from expression pattern

 [TASES] Traceable author statement to experimental support

 [HIBSCS] Human inference based on similarity to consensus sequences

 [BPP] Binding of purified proteins



Reference(s)    

 [1] Brandi A., Pon CL., Gualerzi CO., 1994, Interaction of the main cold shock protein CS7.4 (CspA) of Escherichia coli with the promoter region of hns., Biochimie 76(10-11):1090-8

 [2] Jiang W., Hou Y., Inouye M., 1997, CspA, the major cold-shock protein of Escherichia coli, is an RNA chaperone., J Biol Chem 272(1):196-202

 [3] Jones PG., VanBogelen RA., Neidhardt FC., 1987, Induction of proteins in response to low temperature in Escherichia coli., J Bacteriol 169(5):2092-5

 [4] La Teana A., Brandi A., Falconi M., Spurio R., Pon CL., Gualerzi CO., 1991, Identification of a cold shock transcriptional enhancer of the Escherichia coli gene encoding nucleoid protein H-NS., Proc Natl Acad Sci U S A 88(23):10907-11

 [5] Sharan M., Forstner KU., Eulalio A., Vogel J., 2017, APRICOT: an integrated computational pipeline for the sequence-based identification and characterization of RNA-binding proteins., Nucleic Acids Res 45(11):e96

 [6] Jones PG., Krah R., Tafuri SR., Wolffe AP., 1992, DNA gyrase, CS7.4, and the cold shock response in Escherichia coli., J Bacteriol 174(18):5798-802

 [7] Giangrossi M., Gualerzi CO., Pon CL., 2001, Mutagenesis of the downstream region of the Escherichia coli hns promoter., Biochimie 83(2):251-9

 [8] Goldstein J., Pollitt NS., Inouye M., 1990, Major cold shock protein of Escherichia coli., Proc Natl Acad Sci U S A 87(1):283-7

 [9] Johnston D., Tavano C., Wickner S., Trun N., 2006, Specificity of DNA binding and dimerization by CspE from E. coli., J Biol Chem 281(52):40208-15

 [10] Zere TR., Vakulskas CA., Leng Y., Pannuri A., Potts AH., Dias R., Tang D., Kolaczkowski B., Georgellis D., Ahmer BM., Romeo T., 2015, Genomic Targets and Features of BarA-UvrY (-SirA) Signal Transduction Systems., PLoS One 10(12):e0145035

 [11] Brandi A., Giangrossi M., Giuliodori AM., Falconi M., 2016, An Interplay among FIS, H-NS, and Guanosine Tetraphosphate Modulates Transcription of the Escherichia coli cspA Gene under Physiological Growth Conditions., Front Mol Biosci 3:19

 [12] Tanabe H., Goldstein J., Yang M., Inouye M., 1992, Identification of the promoter region of the Escherichia coli major cold shock gene, cspA., J Bacteriol 174(12):3867-73

 [13] Hankins JS., Zappavigna C., Prud'homme-Genereux A., Mackie GA., 2007, Role of RNA structure and susceptibility to RNase E in regulation of a cold shock mRNA, cspA mRNA., J Bacteriol 189(12):4353-8

 [14] Ivancic T., Jamnik P., Stopar D., 2013, Cold shock CspA and CspB protein production during periodic temperature cycling in Escherichia coli., BMC Res Notes 6:248

 [15] Etchegaray JP., Inouye M., 1999, CspA, CspB, and CspG, major cold shock proteins of Escherichia coli, are induced at low temperature under conditions that completely block protein synthesis., J Bacteriol 181(6):1827-30

 [16] Constantinidou C., Hobman JL., Griffiths L., Patel MD., Penn CW., Cole JA., Overton TW., 2006, A reassessment of the FNR regulon and transcriptomic analysis of the effects of nitrate, nitrite, NarXL, and NarQP as Escherichia coli K12 adapts from aerobic to anaerobic growth., J Biol Chem 281(8):4802-15



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