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DsdC DNA-binding transcriptional dual regulator

Synonyms: DsdC-D-serine
In the presence of d-serine, the "D-serine deaminase" protein, DsdC, activates the transcription of genes involved in d-serine detoxification, and in the absence of the metabolite it is autorepressed [2]. DsbC belongs to the LysR family of bacterial transcriptional regulators and contains a possible helix-turn-helix motif for DNA binding in the C-terminal domain. This protein shows 50% similarity to the GcvA protein [3, 4] The dsbC gene is located next to an operon ( dsdXA ) regulated by DsbC, but facing in the opposite direction. The activation effect of DsdC on this operon is enhanced by CRP, but CRP cannot act alone on the operon [4] A DNA-binding site recognized by the dimer of DsbC has not been identified.

Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
DsdC-D-serine Functional Allosteric Holo [AIFS], [BPP], [GEA], [IEP], [IPI] [1], [2], [3]
Evolutionary Family: LysR
Sensing class: Sensing external and internal signals
Connectivity class: Local Regulator
Gene name: dsdC
  Genome position: 2476694-2477629
  Length: 936 bp / 311 aa
Operon name: dsdC
TU(s) encoding the TF:
Transcription unit        Promoter

Regulated gene(s) dsdA, dsdC, dsdX
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
amino acids (2)
Transcription related (1)
activator (1)
repressor (1)
operon (1)
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Regulated operon(s) dsdC, dsdXA
First gene in the operon(s) dsdA, dsdC, dsdX
Simple and complex regulons CRP,DsdC
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)

Transcription factor regulation    

Transcription factor binding sites (TFBSs) arrangements

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  DsdC-D-serine activator dsdAp Sigma70 nd nd dsdA nd nd [GEA] [4]
  DsdC-D-serine repressor dsdCp Sigma70 nd nd dsdC nd nd [GEA] [2]
  DsdC-D-serine activator dsdXp Sigma70 nd nd dsdX, dsdA nd nd [GEA] [4], [5]

Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


 [AIFS] Automated inference of function from sequence

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [IEP] Inferred from expression pattern

 [IPI] Inferred from physical interaction


 [1] Heincz MC, Bornstein SM, McFall E., 1984, Purification and characterization of D-serine deaminase activator protein., J. Bacteriol.

 [2] McFall E., Heincz MC., 1983, Identification and control of synthesis of the dsdC activator protein., J Bacteriol 153(2):872-7

 [3] Palchaudhuri S., Patel V., McFall E., 1988, DNA sequence of the D-serine deaminase activator gene dsdC., J Bacteriol 170(1):330-4

 [4] Norregaard-Madsen M., McFall E., Valentin-Hansen P., 1995, Organization and transcriptional regulation of the Escherichia coli K-12 D-serine tolerance locus., J Bacteriol 177(22):6456-61

 [5] McFall E., 1986, cis-acting proteins., J Bacteriol 167(2):429-32