RegulonDB RegulonDB 10.9:Regulon Page
   

EbgR DNA-binding transcriptional repressor

Synonyms: EbgR
Summary:
EbgR (for "evolved β-galactosidase repressor") negatively regulates genes involved in the evolved β-galactosidase system, which constitutes an alternative for lactose utilization in cells with mutations on the lacZ gene 3939708. 10234816. The wild-type Ebg enzyme, encoded by the ebg operon, is not efficient enough to permit growth on lactose. Mutations in ebgR and ebgA, however, enhance its catalytic activity and enable rapid growth on lactose as a sole carbon source 3939708. 10234816. EbgR belongs to the GalR-LacI family of transcriptional regulators. There is strong evidence that the ebg and lac operons share a common origin in evolution 3939708. EbgR diverged from other repressors shortly after the divergence of the Gram positive from the Gram negative eubacteria, approximately 2.2 billion years ago 12868605 Reviews: 10234816. 12868605


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
EbgR Functional   [IE] nd
Evolutionary Family: GalR/LacI
Connectivity class: Local Regulator
Gene name: ebgR
  Genome position: 3221466-3222449
  Length: 984 bp / 327 aa
Operon name: ebgR
TU(s) encoding the TF:
Transcription unit        Promoter
ebgR
ebgRp


Regulon       
Regulated gene(s) ebgA, ebgC
Multifun term(s) of regulated gene(s)
carbon compounds (2)
Regulated operon(s) ebgAC
First gene in the operon(s) ebgA
Simple and complex regulons CRP,EbgR
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[EbgR,-](1)


Transcription factor regulation    


Transcription factor binding sites (TFBSs) arrangements
      

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  EbgR repressor ebgAp Sigma70 nd nd ebgA, ebgC
nd
 nd nd [BPP], [GEA], [IGI] [1]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [IE] Inferred from experiment

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [IGI] Inferred from genetic interaction


Reference(s)    

 [1] Hall BG., Betts PW., Wootton JC., 1989, DNA sequence analysis of artificially evolved ebg enzyme and ebg repressor genes., Genetics 123(4):635-48


RegulonDB