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GutR DNA-binding transcriptional repressor

Synonyms: GutR-glucitol, GutR
Summary:
The glucitol repressor, GutR, is a DNA-binding transcription factor that represses an operon (gut) involved in transport and utilization of glucitol [1, 2] This regulator is located in the unusual gut operon, which contains two glucitol-specific transcription factors, GutR and GutM, that regulate this operon negatively and positively, respectively; both regulators control transcription of glucitol PTS permease [1]
Expression of gutR is activated in the presence of glucitol and in the absence of glucose. Although DNA binding by GutM does not depend on the presence of glucitol, this compound appears to be necessary for derepressing gutM, perhaps by interacting with GutR [1]
In addition, Yamada et al. suggested that these regulators have contrary effects, but in the presence of glucitol, GutR interacts with this carbohydrate to dissociate from DNA, causing increments of GutM in sufficient amounts to increase transcription of the gut operon [1] To repress transcription, GutR recognizes DNA-binding sites, but no consensus sequence has been identified.
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Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
GutR Functional   Apo [GEA] [1]
GutR-glucitol Non-Functional Allosteric Holo [GEA] [1]
Evolutionary Family: DeoR
Sensing class: External sensing using transported metabolites
Connectivity class: Local Regulator
Gene name: srlR
  Genome position: 2829047-2829820
  Length: 774 bp / 257 aa
Operon name: srlAEBD-gutM-srlR-gutQ
TU(s) encoding the TF:
Transcription unit        Promoter
srlAEBD-gutM-srlR-gutQ
srlAp
srlR
srlRp


Regulon       
Regulated gene(s) gutM, gutQ, srlA, srlB, srlD, srlE, srlR
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
carbon compounds (6)
Phosphotransferase Systems (PEP-dependent PTS) (3)
Transcription related (2)
activator (1)
core region (1)
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Regulated operon(s) srlAEBD-gutM-srlR-gutQ
First gene in the operon(s) srlA
Simple and complex regulons CRP,GutM,GutR,H-NS
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)


Transcription factor binding sites (TFBSs) arrangements       

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  GutR repressor srlAp Sigma70 nd nd srlA, srlE, srlB, srlD, gutM, srlR, gutQ nd nd [GEA] [1]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [GEA] Gene expression analysis



Reference(s)    

 [1] Yamada M., Saier MH., 1988, Positive and negative regulators for glucitol (gut) operon expression in Escherichia coli., J Mol Biol. 203(3):569-83

 [2] Yamada M., Saier MH., 1987, Physical and genetic characterization of the glucitol operon in Escherichia coli., J Bacteriol. 169(7):2990-4



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