Lysine Regulator, LysR, is negatively autoregulated and coordinately activates transcription of the divergent gene which encodes the enzyme that catalyzes the last step in lysine biosynthesis [1, 2, 3]. Activation by LysR requires the presence of diaminopimelic acid . Stragier et al. have proposed that this compound binds directly with LysR .
LysR belongs to the family LysR, which contains three domains [5, 6]: an amino-terminal domain that contains a potential helix-turn-helix DNA-binding motif; a central domain, with response domains much less conserved, that possibly are important for coinducer-responsive transcription; and a C-terminal domain that includes the key residues possibly involved in DNA interactions and coinducer response . In systematic studies of oligomerization, it was shown that some members of the LysR family, like LysR, interact with other members of the family to form heterodimers, but the physiological significance of this is unknown .
Although little is known about the mechanism of regulation of the LysR transcription factor, Stragier et al. demonstrated that its regulator acts as an activator by binding to cis-acting elements, inverted repeat sequences that are not well-conserved, near the promoter .
|Sensing class:||Sensing external and internal signals|
|Connectivity class:||Local Regulator|
|Length:||936 bp / 311 aa|
|TU(s) encoding the TF:||
|Regulated gene(s)||lysA, lysR|
|Multifun term(s) of regulated gene(s)||
MultiFun Term (List of genes associated to the multifun term)
Transcription related (1)
|Regulated operon(s)||lysA, lysR|
|First gene in the operon(s)||lysA, lysR|
|Simple and complex regulons|
|Simple and complex regulatory phrases||Regulatory phrase (List of promoters regulated by the phrase)|
|Evolutionary conservation of regulatory elements|
 Stragier P., Richaud F., Borne F., Patte JC., 1983, Regulation of diaminopimelate decarboxylase synthesis in Escherichia coli. I. Identification of a lysR gene encoding an activator of the lysA gene., J Mol Biol. 168(2):307-20
 Stragier P., Danos O., Patte JC., 1983, Regulation of diaminopimelate decarboxylase synthesis in Escherichia coli. II. Nucleotide sequence of the lysA gene and its regulatory region., J Mol Biol. 168(2):321-31