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Synonyms: NadR |
Summary:
The multifunctional protein NadR has enzymatic as well as regulatory activity. Initially thought to only function as a transcriptional regulator that represses genes involved in transport and de novo synthesis of NAD [2, 3], NadR was later shown to have nicotinamide mononucleotide (NMN) adenylyltransferase activity [5] and predicted to have ribosylnicotinamide kinase activity [1]. NadR is composed of three different domains: an N-terminal helix-turn-helix domain for DNA binding, a central NMN adenylyltransferase domain that contains an ATP-binding site, and a C-terminal ribosylnicotinamide kinase domain [1]. NadR is often found to be mutated in long-term evolution experiments; interestingly, mutations are usually only found in the N- and C-terminal domains []. It has been proposed that NadR recognizes and binds to two palindromic sequences of 6 bp separated by six less conserved nucleotides. Read more > |
Transcription factor | ![]() ![]() |
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TF conformation(s): |
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Evolutionary Family: | HTH_3 | ||||||||||||
Connectivity class: | Local Regulator | ||||||||||||
Gene name: | nadR | ||||||||||||
Genome position: | 4627315-4628547 | ||||||||||||
Length: | 1233 bp / 410 aa | ||||||||||||
Operon name: | serB-radA-nadR | ||||||||||||
TU(s) encoding the TF: |
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Regulon |
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Regulated gene(s) | nadA, nadB, pncB, pnuC | ||||||
Multifun term(s) of regulated gene(s) |
MultiFun Term (List of genes associated to the multifun term)
nicotinamide adenine dinucleotide (3)
nucleotide and nucleoside conversions (1)
Transporters of Unknown Classification (1)
membrane (1)
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Regulated operon(s) | nadA-pnuC, nadB, pncB | ||||||
First gene in the operon(s) | nadB, pncB, nadA | ||||||
Simple and complex regulons | CRP,NadR NadR | ||||||
Simple and complex regulatory phrases | Regulatory phrase (List of promoters regulated by the phrase) |
Transcription factor regulation |
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Functional conformation | Function | Promoter | Sigma factor | Central Rel-Pos | Distance to first Gene | Genes | Sequence | LeftPos | RightPos | Evidence (Confirmed, Strong, Weak) | References | |
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NadR | repressor | nadAp | nd | -57.5 | -81.5 | nadA, pnuC |
tcgactatctTGTTTAGCATATAAAACAaattacaccg
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781995 | 782012 | [ICWHO], [IMP] | [2], [3] | |
NadR | repressor | nadBp | Sigma70 | 48.5 | -15.5 | nadB |
gaccaaaccaTGTTTAGTAAATTAAACAaagaaaatga
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2710396 | 2710413 | [AIBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA], [IMP] | [2], [3], [4], [5] | |
NadR | repressor | pncBp | Sigma70 | 20.5 | -38.5 | pncB |
ttcctgaagaTGTTTATTGTACTAAACGctcctgtacg
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990386 | 990403 | [AIBSCS] | [3], [4] |
Evolutionary conservation of regulatory elements | ![]() |
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Reference(s) |
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